Construct: ORF ccsbBroadEn_14974
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018890.1_s300c1, BRDN0000458186
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- MAP3K2 (10746)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10746 | MAP3K2 | mitogen-activated protein k... | NM_001371910.2 | 98.6% | 34.5% | (many diffs) |
| 2 | human | 10746 | MAP3K2 | mitogen-activated protein k... | NM_001371911.1 | 98.6% | 34.5% | (many diffs) |
| 3 | human | 10746 | MAP3K2 | mitogen-activated protein k... | NM_006609.5 | 98.6% | 34.5% | (many diffs) |
| 4 | mouse | 26405 | Map3k2 | mitogen-activated protein k... | NM_011946.3 | 90.7% | 32.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 82
- ORF end:
- 841
- ORF length:
- 759
- Sequence:
-
1 CTGATAGTGA CCTGTTCGTT GCAACAAATT GATGAGCAAT GCTTTTTTAT AATGCCAACT 61 TTGTACAAAA AAGTTGGCAC CATGGATGAT CAGCAAGCTT TGAACTCAAT CATGCAAGAT 121 TTGGCTGTCC TTCATAAGGC CAGTCGACCA GCATTATCCT TGCAGGAAAC CAGAAAAGCA 181 AAATCTTCAT CACCAAAAAA ACAGAATGAT GTCCGAGTCA AATTTGAACA TAGAGGAGAA 241 AAAAGAATCC TTCAGTTCCC CAGACCAGTT AAACTGGAAG ATCTGAGATC TAAAGCTAAA 301 ATTGCCTTTG GACAGTCTAT GGATCTACAT TATACCAATA ACGAGTTGGT AATTCCATTA 361 ACTACTCAAG ATGACTTGGA CAAAGCTGTG GAACTGCTGG ATCGTAGTAT TCATATGAAG 421 AGCCTCAAGA TATTACTTGT AATAAATGGA AGTACACAGG CTACTAATTT AGAACCATTG 481 CCATCACTAG AAGATTTGGA TAATACAGTA TTTGGAGCAG AGAGGAAAAA ACGGCTATCT 541 ATAATAGGTC CTACTAGTAG AGATAGAAGT TCTCCTCCCC CAGGTTACAT TCCAGATGAA 601 TTACACCAGG TTGCCCGGAA TGGGTCATTC ACTAGTATCA ACAGTGAAGG AGAGTTCATT 661 CCAGAGAGCA TGGACCAAAT GCTGGATCCA TTATCTTTAA GCAGCCCTGA AAATTCTGGC 721 TCAGAAGTTG TCCATCACTT GATAGTCCTT TGGATGGAGA GAGCTATCCA AAATCACGAA 781 TGCCTAGGGC TCAGAGCTAC CCAGATATCA TCAGATTTTC AGACTATGAT ACCCTATCTT 841 TGAGAATTGG GAAAGAGACA TATCCAGAAG GTATCATGTT TCATATCatc atcaagagta 901 taatgatggt cgtaaaactt ttccaagagc tagaaggacc caggggacca gcttacggtc 961 tcctgtgagt ttcagtccta ctgatcattc cttaagcact agtagtggaa gcagtatctt 1021 taccccagag tatgatgata gtcgaataag aagaagggga agtgacatag acaatcctac 1081 tttgaccgta atggacatca gcccacccag ccgttcacct cgagcTCCGA CCACTTGGAA 1141 GATGGGCAAA CTGCTTGCCC AAGGAGCCTT TGGNAAGGTC TACCTCTGTT ATGATGTGAT 1201 TACAGGAAGA GAATTTGCTG TAAGCAAAGT TCAATTTGAC CCCGATAGTC NTGAGACCAG 1261 CAAGGAAGTA AATGCACTTG AGTGTGAAAT TCAGTTGCTG AAAAACTTGC TACATGAGCG 1321 AATTGTTCAG TATTATGGCT GTTTGAGGGA TCCCCAGGAA AAAACACTTT CCATATTTAT 1381 GGAATATATG CCAGGGGGTT CAATTAAGGA CCAATTAAAA GCATATGGCG CTCTTACTGA 1441 GAATGTGACT AGGAAATACA CCCGTCAGAT TCTGGAGGGT GTCCATTATT TGCACAGTAA 1501 TATGATTGTC CATAGAGATA TCAAAGGCGC AAATATCCTG CGAGATTCAA CAGGCAACGT 1561 CAAACTAGGA GATTTTGGGG CCAGCAAACG GCTTCAGACC ATCTGTCTCT CAGGGACAGG 1621 AATGAAGTCT GTCACGGGCA CACCATACTG GATGAGCCCT GAAGTCATCA GTGGAGAAGG 1681 CTATGGAAGA AAAGCAGACA TCTGGAGTGT TGCATGTACT GTGGTAGAAA TGCTAACTGA 1741 AAAGCCGCCT TGGGCTGAAT TTGAAGCAAT GGCTGCCATC TTTAAAATCG CCACTCAGCC 1801 AACAAACCCA AAGCTGCCAC CTCATGTCTC AGACTATACT CGAGATTTCC TCAAACGGAT 1861 TTTTGTAGAG GCCAAACTGA GACCTTCAGC TGATGAACTC TTAAGGCACA TGTTTGTGCA 1921 TTATCACTTG CCAACTTTCT TGTACAAAGT TGGCATTATA AGAAAGCATT GCTTATCAAT 1981 TTGTTGCAAC GAACAGGTCA CTATCAGTCA AAATAAAATC ATTATTGCCA TCCAGGCTGA 2041 TTCCCCCC