Construct: ORF ccsbBroadEn_15054
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018924.1_s300c1, BRDN0000458220
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- PIK3R4 (30849)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 30849 | PIK3R4 | phosphoinositide-3-kinase r... | NM_014602.3 | 99.5% | 18% | (many diffs) |
| 2 | mouse | 75669 | Pik3r4 | phosphoinositide-3-kinase r... | NM_001081309.1 | 85.3% | 17.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 96
- ORF end:
- 834
- ORF length:
- 738
- Sequence:
-
1 CCGCTTTTTT TTGACTGATA GTGACCTGTT CGTTGCAACA AATTGATGAG CAATGCTTTT 61 TTATAATGCC AACTTTGTAC AAAAAAGTTG GCACCATGGG AAATCAGCTT GCTGGCATTG 121 CTCCCTCCCA GATCCTTTCT GTAGAGAGTT ATTTTTCAGA TATTCATGAC TTTGAATATG 181 ATAAAAGCCT GGGGAGTACT CGGTTTTTTA AAGTTGCTCG AGCCAAGCAC CGAGAAGGCC 241 TGGTCGTTGT GAAGGTTTTT GCAATTCAGG ATCCCACATT GCCTTTAACC AGCTATAAAC 301 AAGAGCTGGA GGAACTGAAA ATCAGGCTTA ATTCTGCACA GAATTGTCTA CCTTTCCAGA 361 AAGCATCAGA AAAAGCATCT GAGAAAGCAG CTATGCTCTT TAGGCAGTAT GTGCGAGACA 421 ATCTCTATGA TCGCATCAGT ACCCGTCCAT TCTTGAATAA CATTGAGAAG CGCTGGATTG 481 CTTTCCAGAT CCTGACAGCT GTGGACCAAG CACACAAATC TGGAGTTCGT CATGGGGACA 541 TCAAGACTGA GAATGTGATG GTCACCAGTT GGAATTGGGT TCTTCTAACT GATTTTGCCA 601 GTTTTAAGCC CACTTATCTT CCAGAAGACA ACCCGGCAGA TTTCAATTAT TTCTTTGACA 661 CATCACGGAG GAGAACTTGC TATATTGCTC CTGAACGTTT TGTTGATGGT GGGATGTTTG 721 CCACTGAGTT AGAATATATG AGAGATCCTT CAACTCCGCT TGTAGACTTA AATAGCAATC 781 AGAGAACAAG AGGAGAGTTG AAGAGAGCAA TGGACATCTT TTCAGCAGTT GTGTGATAGC 841 TGAGCTTTTT ACAGAAGTGT ACCATTATTT GATCTCTCTC AACTTTTGGC TTATAGAAAT 901 GGACATTTTT TCCCTGAaca agtgctaaat aaaattgaag atcacagtat cagagaattg 961 gtaactcaga tgattcaccg tgagccagat aaacgtttag aggcagaaga ttacttaaaa 1021 cagcagcgtg gcaatgcctt tcctgaaata ttttacactt ttcttcagcc ctacatggcc 1081 cagtttgcca aggaaacgtt tctttctgca gatgagcgta ttctggttat acggaaggat 1141 ttgggcaaca ttattcacaa tctctgtgga catgatctgc cagaaaaagc cgaaggagag 1201 cctaaggaaa atgggctggt tatcttggta tctgttataa catcctgcct acagaccctt 1261 aaatactgtg attccaaact agctgctttg gaactgattc ttcatttggc tccaagatta 1321 agtgttgaaa tccttttgga tcgtattact ccatatcttt tgcatttcag caatgactct 1381 gttcctaggg tgagggctga agccttgagg acgttgacca aagttcttgc tctcgtcaaa 1441 gaggttcctc gtaatgatat caatatttat ccggaataca ttctgccagg catagcccac 1501 ttagcccaag atgatgctac tatcgttaga ctagcctatg ctgaaaacat agctctgctg 1561 gcagaaacag ctctgagatt cctggaatta gtacagttaa aaaatcttaa tatggaaaat 1621 gaccccaata atgaagaaat agatgaggtt acacatccaa atggaaatta tgacacagag 1681 ctccaagcct tacatgaaat ggtccagcag aaagttgtta ctttgctaag tgaccctgaa 1741 aatattgtaa aacaaacctt gatggaaaat ggaataacac ggctgtgtgt attctttgga 1801 cgtcagaaag ccaacgatgt tttgttgtcc cacatgatta ctttcctaaa tgataagaat 1861 gattggcatc tacgtggagc attttttgat agtatagttg gtgttgctgc ctatgttggc 1921 tggcaaagct cctcaattct caagcctctg ctgcaacaag gtcttagtga tgctgaggaa 1981 tttgtcattg tgaaagctct ttatgccctt acttgtatgt gccagttagg actgctacaa 2041 aaaccccatg tttacgaatt tgccagtgat attgccccct tcctgtgtca tcccaattta 2101 tggatacgtt atggtgccgt gggatttatc acagtggtag ctcgtcaaat aagtacagct 2161 gatgtctact gtaaactgat gccttatctt gacccatata ttacccaacc aataatacag 2221 attgaaagaa aacttgttct gctcagtgtt ttaaaggaac cagtaagtcg ttctatattt 2281 gattatgctt tgaggtctaa agatattact agcttgttca gacatcttca catgcgtcag 2341 aagaaacgaa atggttctct tcccgactgc cctccgccag aggatcctgc catagcacag 2401 cttctgaaga agttgctctc acagggaatg acagaggaag aggaagacaa acttctggca 2461 ctgaaagact tcatgatgaa atctaataaa gcaaaggcca atatagtgga ccagagccat 2521 cttcatgata gtagtcagaa aggtgtaatt gacttggcag ctttaggcat aactgggaga 2581 caagttgatc ttgttaaaac caaacaagaa ccagatgaca aacgggccag aaaacatgta 2641 aaacaagact caaatgtaaa tgaagaatgg aaaagcatgt ttgggtcact ggacccacca 2701 aacatgccac aggccctacc taaagggagt gatcaggagg tgattcagac tgggaaacct 2761 cctcgttccg agtcctctgc tggcatttgt gtccctttgt caacttcttc acaggttcca 2821 gaagtgacaa ctgtccaaaa taaaaaacca gtaataccgg ttttaagtag tacaatctta 2881 ccatccacct atcagattcg aattacaact tgtaaaactg aacttcagca actcatccag 2941 caaaagcggg agcagtgcaa tgctgagaga atagctaagc agatgatgga aaatgctgaa 3001 tgggagagta aaccaccacc acctggatgg cgtcctaaag ggctgttagt tgcccatctt 3061 catgagcata aatctgctgt gaatcgaatt agagtctctg atgaacactc actttttgca 3121 acatgttcaa atgatggcac agtgaaaatc tggaacagtc aaaagatgga ggggaagacc 3181 accactacca gatctattct tacatacagc cgaattggag gacgagtcaa gacgctcaca 3241 ttctgccaag gctcccacta tttagccata gcatctgata atggtgctgt ccagcttctt 3301 ggaattgagg cttctaagct gcccaagtct cctaaaatcc atcctctaca aagcagaatt 3361 ctagaTCAGA AGGAGGACGG GTGTGTTGTG GATATGCATC ACTTCACTTC TGGAGCACAG 3421 TCTGTTCTGC NNTATGCCAC TGTGAATGGC TCTCTGGTNG GCTGGACCNT AGGGTCTCAA 3481 AGCAATGCGT GGACTTAAAA GCATGATTTA AAGTCGGGCC TCATCACTTC CTTTGCTGTG 3541 GACATCCACC CAATGCTGGC TCTGCATTGG TACAAGCAGT GGTACCATGG CTTGTTGGGA 3601 CATGAGGTTC CAGTTGCCAA TTTCAAGTCA CTGTCATCCT TCCAGGGCTC GAATCAGACG 3661 CCTCTCAATG CACCCTCTGT ATCAGTCCTG GGTGATTGCA GCTGTTCAGG GCAACAACGA 3721 AGTGTCCATG TGGGACATGG AGACTGGTGA CAGAAGATTT ACTCTCTGGG CCAGCAGTGC 3781 ACCACCACTT TCTGAATTAC AGCCTTCTCC TCATAGCGTC CATGGTATCT ACTGTAGTCC 3841 TGCAGATGGA AATCCTATCC TACTAACAGC TGGCTCAGAT ATGAAAATAA GGTTTTGGGA 3901 CTTGGCTTAC CCAGAAAGGT CCTATGTTGT TGCAGGAAGT ACTAGTTCCC CATCTGTGTC 3961 CTACTACAGG AAAATAATTG AAGGCACTGA AGTTGTCCAG GAAATTCAGA ATAAGCAGAA 4021 AGTAGGACCA AGTGATGACA CCCCTCGAAG GGGCCCAGAG TCCCTGCCCG TGGGACATCA 4081 TGACATCATC ACTGATGTCG CCACATTCCA GACCACACAG GGCTTCATCG TAACTGCTTC 4141 TAGAGATGGG ATTGTGAAGG TGTGGAAATT GCCAACTTTC TTGTACAAAG TTGGCATTAT 4201 AAGAAAGCAT TGCTTATCAA TTTGTTGCAA CGAACAGGTC ACTATCAGTC AAAATAAAAT 4261 CATTATTGCC ATCCCAGCCT GATA