Construct: ORF ccsbBroadEn_15059
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018905.1_s300c1, BRDN0000458201
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- TNNI3K (51086)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51086 | TNNI3K | TNNI3 interacting kinase | NM_015978.3 | 99.5% | 70.2% | (many diffs) |
| 2 | human | 100526835 | FPGT-TNNI3K | FPGT-TNNI3K readthrough | NM_001112808.2 | 87.1% | 60.7% | (many diffs) |
| 3 | human | 100526835 | FPGT-TNNI3K | FPGT-TNNI3K readthrough | NM_001199327.1 | 76% | 67.2% | (many diffs) |
| 4 | mouse | 435766 | Tnni3k | TNNI3 interacting kinase | NM_177066.5 | 84.6% | 64.5% | (many diffs) |
| 5 | mouse | 435766 | Tnni3k | TNNI3 interacting kinase | XM_011240154.1 | 73.6% | 73.7% | (many diffs) |
| 6 | mouse | 435766 | Tnni3k | TNNI3 interacting kinase | XM_011240155.1 | 73.6% | 73.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 97
- ORF end:
- 1861
- ORF length:
- 1764
- Sequence:
-
1 ACTGATTTAT TTTGACTGAT AGTGACCTGT TCGTTGCAAC AAATTGATGA GCAATGCTTT 61 TTTATAATGC CAACTTTGTA CAAAAAAGTT GGCACCATGG GAAATTATAA ATCTAGACCA 121 ACCCAAACTT GTACTGATGA ATGGAAGAAA AAAGTCAGTG AATCATATGT TATCACAATA 181 GAAAGATTAG AAGATGACCT GCAGATCAAG GAAAAAGAAC TGACAGAACT AAGGAATATA 241 TTTGGCTCTG ATGAAGCCTT CAGTAAAGTC AATTTAAATT ACCGCACTGA AAATGGGCTG 301 TCTCTACTTC ATTTATGTTG CATTTGTGGA GGCAAGAAAT CACATATTCG AACTCTTATG 361 TTGAAAGGGC TCCGCCCATC TCGACTGACA AGAAATGGAT TTACAGCCTT GCATTTAGCA 421 GTTTACAAGG ATAATGCAGA ATTGATCACT TCTCTGCTTC ACAGTGGAGC TGATATACAG 481 CAGGTTGGAT ACGGTGGCCT CACTGCCCTC CATATTGCTA CAATAGCTGG CCACCTAGAG 541 GCTGCTGATG TGCTGTTGCA ACATGGAGCT AATGTCAATA TTCAAGATGC AGTTTTTTTC 601 ACTCCATTGC ATATTGCAGC GTACTATGGA CATGAACAGG TAACTCGCCT TCTTTTGAAA 661 TTTGGTGCTG ATGTAAATGT AAGTGGTGAA GTTGGAGATA GACCCCTCCA CCTAGCATCT 721 GCAAAAGGAT TCTTGAATAT TGCAAAACTC TTGATGGAAG AACGCAGCAA AGCAGATGTG 781 AATGCTCAAG ATAATGAAGA CCATGTCCCA CTCCATTTCT GTTCTCGATT TGGACACCAT 841 GATATAGTTA AGTATCTGCT GCAAAGTGAT TTGGAAGTTC AACCTCatgt tgttaatatc 901 tatggagata cccccttaca cctggcatgc tacaatggca aatttgaagt tgccaaggaa 961 atcatccaaa tatcaggaac agaaagtctg actaaggaaa acatcttcag tgaaacagct 1021 tttcatagtg cttgtaccta tggcaagagc attgacctag tcaaatttct tcttgatcag 1081 aatgtcataa acatcaacca ccaaggaagg gatgggcaca ctggattaca ctctgcttgc 1141 taccacggtc acattcgcct ggttcagttc ttactggata atggagctga tatgaatcta 1201 gtggcttgtg atcccagcag gtctagtggt gaaaaagatg agcagacatg tttgatgtgg 1261 gcttatgaaa aagggcatga tgccattgtc acactcctga agcattataa gagaccacaa 1321 gatgaattgc cctgtaatga atattctcag cctggaggag atggctccta tgtgtctgtt 1381 ccatcaccct tggggaagat taaaagcatg acaaaagaga aggcagatat tctcctccta 1441 agagctggat tgccttcaca tttccatctt cagctctcag aaattgagtt ccatgagatt 1501 attggctcag gttcttttgg gaaagtatat aaaggacgat gcagaaataa aatagtggct 1561 ataaaacgtt atcgagccaa tacctactgc tccaagtcag atgtggatat gttttgccga 1621 gaggtgtcca ttctctgcca gctcaatcat ccctgcgtaa ttcagtttgt gggtgcttgc 1681 ttgaatgatc ccagccagtt tgccattgtc actcaataca tatcaggggg ttctctgttc 1741 tccctccttc atgagcagaa gaggattctt gatttgcagt ctaaattaat tattgcagta 1801 gatgttgcca aaggcatgga gtaccttcac aacCTGACAC AGCCAATTAT ACATCGTGAC 1861 TGAAACAGTC ACATATTTCT TTCTCTATGA GGATGGGCAT GCTGTGTGGG CAGATTTTGA 1921 GAAATCAAGA TTTCTACAGT CTCTGGATGA AGACAACATG ACAAAACAAC CTGGGAACCT 1981 CCGTTGGATG GCTCNTGAGG TGTTCACGCA GTGCACTCGG TACACCATCA AAGCAGATGT 2041 CTTCAGCTAT GCTCTGTGTC TGTGGGAAAT TCTCACTGGC GAAATTCCAT TCGCTCATCT 2101 CAAGCCAGCG GCTGCGGCAG CAGACATGGC TTACCACCAC ATCAGACCTC CCATTGGCTA 2161 TTCCATTCCC AAGCCCATAT CATCTCTGCT GATACGAGGG TGGAACGCAT GTCCTGAAGG 2221 AAGACCCGAA TTTTCTGAAG TTGTCATGAA GTTAGAAGAG TGTCTCTGCA ACATTGAGCT 2281 GATGTCTCCT GCATCAAGTA ACAGCAGTGG GTCTCTCTCA CCTTCTTCTT CTTCTGATTG 2341 CCTGGTGAAC CGGGGAGGAC CTGGCCGGAG TCATGTGGCA GCATTAAGAA GTCGTTTCGA 2401 ATTGGAATAT GCTCTAAATG CAAGGTCCTA TGCTGCTTTG TCCCAAAGTG CTGGACAATA 2461 TTCCTCTCAA GGTCTGTCTT TGGAGGAGAT GAAAAGAAGT CTTCAATACA CACCCATTGA 2521 CAAATATGGC TATGTATCCG ATCCCATGAG CTCAATGCAT TTTCATTCTT GCCGAAATAG 2581 TAGCAGCTTT GAGGACAGCA GCTTGCCAAC TTTCTTGTAC AAAGTTGGCA TTATAAGAAA 2641 GCATTGCTTA TCAATTTGTT GCAACGAACA GGTCACTATC AGTCAAAATA AAATCATTAT 2701 TGCCATCCCA GCTGAAATT