Construct: ORF ccsbBroadEn_15098
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018939.1_s300c1, BRDN0000458235
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- PI4K2A (55361)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55361 | PI4K2A | phosphatidylinositol 4-kina... | NM_018425.3 | 90.8% | 44.4% | 164_292del;767_768insG;823_824insCG |
| 2 | mouse | 84095 | Pi4k2a | phosphatidylinositol 4-kina... | NM_145501.2 | 84% | 42.3% | (many diffs) |
| 3 | mouse | 84095 | Pi4k2a | phosphatidylinositol 4-kina... | XM_011247405.2 | 84% | 42.3% | (many diffs) |
| 4 | mouse | 84095 | Pi4k2a | phosphatidylinositol 4-kina... | XM_011247406.2 | 80.2% | 44.2% | (many diffs) |
| 5 | mouse | 84095 | Pi4k2a | phosphatidylinositol 4-kina... | XR_877696.2 | 52.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 100
- ORF end:
- 763
- ORF length:
- 663
- Sequence:
-
1 CAAATGATTT TATTTTGACT GATAGTGACC TGTTCGTTGC AACAAATTGA TGAGCAATGC 61 TTTTTTATAA TGCCAACTTT GTACAAAAAA GTTGGCACCA TGGACGAGAC GAGCCCACTA 121 GTGTCCCCCG AGCGGGCCCA ACCCCCGGAC TACACCTTCC CGTCGGGCTC GGGCGCTCAC 181 TTTCCGCAGG TGCCCGGGGG CGCGGTCCGA GTGGCGGCGG CGGCCGGCTC GGGCCCCTCT 241 CCGCCGGGCT CGCCGGGCCA CGACCGCGAG CGGAACGAGT TCCCGGAGGA TCCTGAGTTC 301 GAGGCGGTGG TGCGGCAGGC CGAGCTGGCC ATCGAGCGCT GCATCTTTCC CGAGCGCATC 361 TACCAGGGCT CCAGCGGAAG CTACTTCGTC AAGGACCCTC AGGGGAGGAT CATTGCTGTC 421 TTCAAACCCA AGAATGAAGA GCCCTATGGG CATCTTAATC CTAAGTGGAC CAAGTGGCTG 481 CAGAAGCTGT GCTGTCCTTG CTGCTTTGGC CGTGACTGCC TTGTCCTTAA CCAGGGCTAT 541 CTCTCAGAAG CAGGGGCCAG CCTGGTGGAC CAAAAACTGG AACTCAACAT TGTTCCCCGT 601 ACAAAGGTAG TATACCTGGC CAGTGAGACC TTCAACTATA GTGCCATTGA CCGAGTGAAG 661 TCCAGGGGCA AGCGGCTTGC ACTAGAGAAA GTGCCAAAAG TTGGACAGCG GTTTAACCGC 721 ATCGGGCTAC CACCAAAGGG TTGGTTCATT CCAGCTCTTT GTTGAAGGCT ACAAAGATGC 781 AGACTATTGG CTGCCGGGCG TTTTGAAGCA GAACCTCTTC CTGAGAACAC TAACCGGCAA 841 CTACTGCTCC AGTTTGAGCG GTTGGTGGTG CTGGATTACA TCATCCGCAA CACTGATCGA 901 GGCAATGACA ACTGGCTGAT TAAATATGAC TGTCCAATGG ATAGTTCTAG CTCTCGGGAC 961 ACAGACTGGG TGGTGGTGAA GGAGCCTGTT ATCAAGGTGG CTGCCATAGA CAATGGGCTG 1021 GCCTTCCCAC TGAAGCATCC TGACTCCTGG AGGGCATATC CTTTTTACTG GGCCTGGTTG 1081 CCCCAGGCGA AAGTCCCATT TTCTCAGGAG ATCAAAGATC TGATCCTTCC AAAGATATCG 1141 GACCCTAACT TCGTCAAGGA CTTGGAAGAG GACCTATATG AACTCTTCAA GAAAGATCCT 1201 GGTTTCGACA GGGGCCAGTT CCATAAGCAG ATTGCTGTCA TGCGGGGCCA GATCTTAAAT 1261 CTGACCCAGG CCTTGAAAGA CAACAAGAGT CCCCTGCACC TCGTCCAGAT GCCACCTGTG 1321 ATTGTCGAGA CGGCCCGTTC CCACCAGCGG TCTTCTAGCG AGTCCTACAC ACAGAGCTTT 1381 CAGAGCCGGA AGCCCTTCTT TTCATGGTGG TTGCCAACTT TCTTGTACAA AGTTGGCATT 1441 ATAAGAAAGC ATTGCTTATC AATTTGTTGC AACGAACAGG TCACTATCAG TCAAAATAAA 1501 ATCATTATTG CCATCCAGCT GATCAGTG