Construct: ORF ccsbBroadEn_15106
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018894.1_s300c1, BRDN0000458190
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- SCYL2 (55681)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55681 | SCYL2 | SCY1 like pseudokinase 2 | NM_001317784.1 | 99.7% | 74.9% | (many diffs) |
| 2 | human | 55681 | SCYL2 | SCY1 like pseudokinase 2 | NM_017988.6 | 99.7% | 74.9% | (many diffs) |
| 3 | human | 55681 | SCYL2 | SCY1 like pseudokinase 2 | NM_001330253.1 | 99.2% | 74.5% | (many diffs) |
| 4 | human | 55681 | SCYL2 | SCY1 like pseudokinase 2 | NM_001330254.1 | 99.2% | 74.5% | (many diffs) |
| 5 | human | 55681 | SCYL2 | SCY1 like pseudokinase 2 | NM_001330256.1 | 80.7% | 56% | (many diffs) |
| 6 | mouse | 213326 | Scyl2 | SCY1-like 2 (S. cerevisiae) | NM_198021.2 | 88.6% | 71.7% | (many diffs) |
| 7 | mouse | 213326 | Scyl2 | SCY1-like 2 (S. cerevisiae) | NM_001310704.1 | 88.5% | 71.6% | (many diffs) |
| 8 | mouse | 213326 | Scyl2 | SCY1-like 2 (S. cerevisiae) | XM_011243394.2 | 88.2% | 71.4% | (many diffs) |
| 9 | mouse | 213326 | Scyl2 | SCY1-like 2 (S. cerevisiae) | XM_006513458.3 | 88.1% | 71.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 97
- ORF end:
- 2203
- ORF length:
- 2106
- Sequence:
-
1 ATCGATTTAT TTTGACTGAT AGTGACCTGT TCGTTGCAAC AAATTGATGA GCAATGCTTT 61 TTTATAATGC CAACTTTGTA CAAAAAAGTT GGCACCATGG AGTCCATGCT TAATAAATTG 121 AAGAGTACTG TTACAAAAGT AACAGCTGAT GTCACTAGTG CTGTAATGGG AAATCCTGTC 181 ACTAGAGAAT TTGATGTTGG TCGACACATT GCCAGTGGTG GCAATGGGCT AGCTTGGAAG 241 ATTTTTAATG GCACAAAAAA GTCAACAAAG CAGGAAGTGG CAGTTTTTGT CTTTGATAAA 301 AAACTGATTG ACAAGTATCA AAAATTTGAA AAGGATCAAA TCATTGATTC TCTAAAACGA 361 GGAGTCCAAC AGTTAACTCG GCTTCGACAC CCTCGACTTC TTACTGTCCA GCATCCTTTA 421 GAAGAATCCA GGGATTGCTT GGCATTTTGT ACAGAACCAG TTTTTGCCAG TTTAGCCAAT 481 GTTCTTGGTA ACTGGGAAAA TCTACCTTCC CCTATATCTC CAGACATTAA GGATTATAAA 541 CTTTATGATG TAGAAACCAA ATATGGTTTG CTTCAGGTTT CTGAAGGATT GTCATTCTTG 601 CATAGCAGTG TGAAAATGGT GCATGGAAAT ATCACTCCTG AAAATATAAT TTTGAATAAA 661 AGTGGAGCCT GGAAAATAAT GGGTTTTGAT TTTTGTGTAT CATCAACCAA TCCTTCTGAA 721 CAAGAGCCTA AATTTCCTTG TAAAGAATGG GACCCAAATT TACCTTCATT GTGTCTTCCA 781 AATCCTGAAT ATTTGGCTCC TGAATACATA CTTTCTGTGA GCTGTGAAAC AGCCAGTGAT 841 ATGTATTCTT TAGGAACTGT TATGTATGCT GTATTTAATA aagggaaacc tatatttgaa 901 gtcaacaagc aagatattta caagagtttc agtaggcagt tggatcagtt gagtcgttta 961 ggatctagtt cacttacaaa tatacctgag gaagttcgtg aacatgtaaa gctactgtta 1021 aatgtaactc cgactgtaag accagatgca gatcaaatga caaagattcc cttctttgat 1081 gatgttggtg cagtaacact gcaatatttt gataccttat tccaaagaga taatcttcag 1141 aaatcacagt ttttcaaagg actgccaaag gttctaccaa aactgcccaa gcgtgtcatt 1201 gtgcagagaa ttttgccttg tttgacttca gaatttgtaa accctgacat ggtacctttt 1261 gttttgccca atgttctact tattgctgag gaatgcacca aagaagaata tgtcaaatta 1321 attcttcctg aacttggccc tgtgtttaag cagcaggagc caatccagat tttgttaatt 1381 ttcctacaaa aaatggattt gctactaacc aaaacccctc ctgatgagat aaagaacagt 1441 gttctaccca tggtttacag agcactagaa gctccttcca ttcagatcca ggagctctgt 1501 ctaaacatca ttccaacctt tgcaaatctt atagactacc catccatgaa aaacgctttg 1561 ataccaagaa ttaaaaatgc ttgtctacaa acatcttccc ttgcggttcg tgtaaattca 1621 ttagtgtgct taggaaagat tttggaatac ttggataagt ggtttgtact tgatgatatc 1681 ctacccttct tacaacaaat tccatccaag gaacctgcgg tcctcatggg aattttaggt 1741 atttacaaat gtacttttac tcataagaag ttgggaatca ccaaagagca gctggccgga 1801 aaagtgttgc ctcatcttat tcccctgagt attgaaaaca atcttaatct taatcagttc 1861 aattctttca tttccgtcat aaaagaaatg cttaatagat tggagtctga acataagact 1921 aaactggagc aacttcatat aatgcaagaa cagcagaaat ctttggatat aggaaatcaa 1981 atgaatgttt ctgaggagat gaaagttaca aatattggga atcagcaaat tgacaaagtt 2041 tttaacaaca ttggagcaga ccttctgact ggcagtgagt ccgaaaataa agaggacggg 2101 tACCGGAATA ACCATAAAAG AGCATCACTT ACACCTGAAG AAAAACAAAA ATTAGCAAAA 2161 GAACAAGAGC AGGCACAGAA GCTGAAAAGC CCAGCAGCCT CTTAAACCCC CAAGTGCACA 2221 CACCTGTTGC TACTGTTAAA CAGACTAAGG ACTTGACAGA CACATTGATG GATAATATGT 2281 CATCCTTGAC CAGCCTTTCT GTTAGTACCC CTAAATCTTC TGCTTCAAGT ACTTTCACTT 2341 CTGTTCCTTC CATGGGCATT GGTATGATGT TTTCTACACC AACTGATAAT ACAAAGAGAA 2401 ATTTGACAAA TGGCCTAAAT GCCAATATGG GCTTTCAGAC TTCAGGATTC AACATGCCCG 2461 TTAATACAAA CCAGAACTTC TACAGTAGTC CAAGCACAGT TGGAGTGACC AAGATGACTC 2521 TGGGAACACC TCCCACTTTG CCAAACTTCA ATGCTTTGAG TGTTCCTCCT GCTGGTGCAA 2581 AGCAGACCCA ACAAAGACCC ACAGATATGT CTGCCCTTAA TAATCTCTTT GGCCCTCAGA 2641 AACCCAAAGT TAGCATGAAC CAGTTATCAC AACAGAAACC AAATCAGTGG CTTAATCAGT 2701 TTGTACCTCC TCAAGGTTCT CCAACTATGG GCAGTTCAGT AATGGGGACA CAGATGAACG 2761 TGATAGGACA ATCTGCTTTT GGTATGCAGG GTAATCCTTT CTTTAACCCA CAGAACTTTG 2821 CACAGCCACC AACTACTATG ACCAATAGCA GTTCAGCTAG CAATGATTTA AAAGATCTTT 2881 TTGGGTTGCC AACTTTCTTG TACAAAGTTG GCATTATAAG AAAGCATTGC TTATCAATTT 2941 GTTGCAACGA ACAGGTCACT ATCAGTCAAA ATAAAATCAT TATTGCCATC CAGGCTGATT 3001 CCCCCCC