Construct: ORF ccsbBroadEn_15109
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018970.1_s300c1, BRDN0000458266
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RIOK2 (55781)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55781 | RIOK2 | RIO kinase 2 | NM_018343.3 | 99.2% | 95.6% | (many diffs) |
| 2 | human | 55781 | RIOK2 | RIO kinase 2 | NM_001159749.2 | 84.6% | 80.4% | (many diffs) |
| 3 | human | 55781 | RIOK2 | RIO kinase 2 | XM_017009628.1 | 65.4% | 61.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 95
- ORF end:
- 1748
- ORF length:
- 1653
- Sequence:
-
1 AATGATTTTA TTTTGACTGA TAGTGACCTG TTCGTTGCAA CAAATTGATG AGCAATGCTT 61 TTTTATAATG CCAACTTTGT ACAAAAAAGT TGGCATGGGG AAAGTGAATG TGGCCAAGTT 121 GCGTTACATG AGCCGAGATG ACTTCAGGGT CTTGACCGCG GTTGAAATGG GCATGAAGAA 181 CCATGAAATT GTTCCCGGCA GTTTGATTGC TTCTATAGCC AGCCTTAAAC ATGGTGGCTG 241 TAATAAAGTT TTAAGAGAAT TAGTGAAACA TAAACTCATA GCTTGGGAGC GTACCAAAAC 301 TGTCCAGGGC TATCGGTTGA CAAATGCAGG ATATGATTAC CTAGCTTTGA AAACACTTTC 361 TTCTAGGCAA GTAGTTGAGT CTGTTGGAAA CCAGATGGGT GTTGGCAAAG AATCAGATAT 421 TTACATTGTT GCAAATGAAG AAGGACAACA ATTTGCATTA AAGCTTCACA GACTAGGAAG 481 AACCTCGTTT CGAAATTTGA AAAACAAACG CGATTATCAT AAATATAGGC ACAATGTGTC 541 ATGGCTATAT TTATCTCGTC TCTCTGCCAT GAAGGAATTT GCCTATATGA AGGCATTGTA 601 TGAGAGGAAA TTTCCAGTTC CAAAGCCAAT TGATTACAAT CGTCATGCAG TGGTCATGGA 661 ACTCATAAAT GGTTATCCAC TATGTCAGAT ACACCATGTT GAAGATCCTG CATCAGTATA 721 TGATGAAGCT ATGGAACTAA TTGTCAAACT TGCAAATCAT GGGCTGATTC ATGGAGATTT 781 TAATGAATTT AATCTCATTT TGGATGAAAG TGACCATATC ACCATGATTG ATTTTCCACA 841 GATGGTTTCA ACTTCTCATC CCAATGCTGA GTGGTATTTT GAACAGGAGA Aatgcattaa 901 agatttcttt atgaaacgtt tcagctacga aagtgagctt tttccaactt ttaaggatat 961 cAGAAGAGAA GACACTCTTG ATGTGGAGTT TCTGCCAGTG GCTACACAAA GGAAATGCAG 1021 GCAGATGATG AACTGCTTCA TCCCATTAGG TCCAGATGAT AAAAATATTG AACAAAAAGA 1081 GGGATCTGAA TTCTCATTTT CAGATGGAGA AGTGGCAGAA AAAGCAGAGG TTTACGGGTC 1141 AGAAAATGAA AGTGAACGGA ACTGTCTAGA AGAATCAGAG GGCTGCTATT GCAGATCATC 1201 TGGAGACCCT GAACAAATAA AGGAAGACAG TTTATCAGAA GAGAGTGCTG ATGCACGGAG 1261 TTTTGAAATG ACTGAATTCA ATCAAGCTTT AGAAGAAATA AAAGGGCAGG TTGTTGAAAA 1321 CAACTCTGTA ACTGAATTTT CTGAGGAGAA AAACAGAACT GAAAATTACA ACAGGCAAGA 1381 TGGTCAGAGA GTTCAAGGAG GAGTCCCTGC TGGCTCTGAC GAGTATGAAG ATGAATGCCC 1441 TCATCTAATT GCCTTGTCGT CATTAAATAG AGAATTCAGG CCTTTCAGAG ATGAAGAAAA 1501 TGTGGGAGCT ATGAATCAGT ATAGAACAAG AACTCTGAGT ATCACTTCTT CAGGCAGTGC 1561 TGTAAGCTGT TCAACAATTC CTCCAGAACT GGTGAAACAG AAGGTGAAAC GTCAGTTGAC 1621 AAAACAGCAA AAATCAGCTG TCAGACGTCG ATTGCAGAAA GGAGAAGCAA ATATATTTAC 1681 CAAGCAACGT AGGGAAAACA TGCAAAATAT CAAATCAAGT TTGGAAGCAG CCAGCTTTTG 1741 GGGAGAATTG CCAACTTTCT TGTACAAAGT TGGCATTATA AGAAAGCATT GCTTATCAAT 1801 TTGTTGCAAC GAACAGGTCA CTATCAGTCA AAATAAAATC ATTATTGCCA TCCCAGCTGA 1861 TATT