Construct: ORF ccsbBroadEn_15150
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014617.2_s300c1, BRDN0000458335
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FASTKD1 (79675)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79675 | FASTKD1 | FAST kinase domains 1 | XM_017004911.2 | 99.9% | 100% | 1161A>G |
| 2 | human | 79675 | FASTKD1 | FAST kinase domains 1 | NM_001322048.1 | 81.8% | 81.9% | 1_447del;1608A>G |
| 3 | human | 79675 | FASTKD1 | FAST kinase domains 1 | XM_006712750.4 | 81.8% | 81.9% | 1_447del;1608A>G |
| 4 | human | 79675 | FASTKD1 | FAST kinase domains 1 | NM_001322046.1 | 79.6% | 79.6% | 1_516del;1677A>G |
| 5 | human | 79675 | FASTKD1 | FAST kinase domains 1 | NM_024622.6 | 79.6% | 79.6% | 1_516del;1677A>G |
| 6 | human | 79675 | FASTKD1 | FAST kinase domains 1 | XM_006712751.4 | 77.7% | 77.4% | (many diffs) |
| 7 | human | 79675 | FASTKD1 | FAST kinase domains 1 | NM_001322049.1 | 76.6% | 76.5% | 1_447del;1608A>G;1876_1877ins129 |
| 8 | human | 79675 | FASTKD1 | FAST kinase domains 1 | NM_001281476.2 | 74.5% | 74.4% | 1_516del;1677A>G;1945_1946ins129 |
| 9 | human | 79675 | FASTKD1 | FAST kinase domains 1 | XM_017004912.2 | 71.3% | 70.9% | (many diffs) |
| 10 | human | 79675 | FASTKD1 | FAST kinase domains 1 | NR_104020.2 | 44.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2091
- ORF length:
- 2025
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaactttg tacaaaaaag 61 ttggcatggg aaaaatagct gatattgttc ataggaactt ggaaaccaca caggacttaa 121 gttccttgtc tgtcttgatg gtcaacatat cttctttaat atcacgacat tttcaacaac 181 aactggtgaa caaaacagaa cttctttttg acaccataga ttcttctgag gtcaacgttg 241 caaaaagcat agcaaagttt cttcgaaatg ttagatatcg ttatcaacca ctattagaaa 301 gatgtaataa cgtattttta agtaatgtgg accaccttga tttggattcc atcagtaaaa 361 tacttagtgt atacaaattt ctacaattta atagttttga atttattata atggctaaaa 421 agaagctaac tgaaatgatt cctctgtgta atcatcctgc tagctttgta aaattgtttg 481 tagcattggg acccattgca ggacctgaag aaaagaaaca acttaaatca actatgttat 541 tgatgtcaga ggacctaact ggcgagcaag ccctggcagt gttgggagca atgggagata 601 tggaaagcag aaactcatgt ctgattaaaa gagttacttc agttctgcat aaacatttgg 661 atggctataa accattagag ttgttgaaga taactcaaga attaactttt ctgcatttcc 721 aaaggaagga gttttttgcg aaacttagag aattactgct tagttatttg aaaaatagtt 781 tcataccaac tgaggtgtct gttctggtcc gtgctatttc cctgctccct tctcctcact 841 tggacgaagt ggggatatcc cgaattgaag ccgttttacc acagtgtgac ctaaataacc 901 tgagtagttt tgccacatct gttttaagat ggattcagca tgatcacatg tatttggata 961 atatgactgc gaaacaactg aaactacttc aaaaattaga tcactatggt cgtcagagac 1021 tacaacacag caacagtttg gatctgttac ggaaggaact taaatctctc aaaggaaaca 1081 cgtttcctga gtcacttctt gaagaaatga ttgctacttt acagcatttc atggatgata 1141 ttaattacat aaatgttggg gagattgcat cttttatttc tagtactgat tacctcagta 1201 ctttgctact agataggata gcctcggtgg ctgttcagca gattgaaaag atccatcctt 1261 ttacaatccc tgctattatt cgtccattca gcgtattgaa ctatgatcca cctcaaaggg 1321 atgaattttt gggaacttgc gtgcaacatc ttaattctta cttaggtata ttggatcctt 1381 ttatattagt gtttcttggt ttctctttgg ccacacttga atattttcca gaagatctgc 1441 taaaggcaat ttttaacatc aaattcttag ctagattgga ttctcaactt gaaattttat 1501 ctccatctcg aagtgcaaga gtccagtttc atcttatgga gttaaataga tcagtctgct 1561 tggaatgccc tgagtttcag attccatggt ttcatgaccg cttctgtcaa caatataata 1621 aaggtattgg tggcatggat ggaacacaac agcagatttt taaaatgtta gcagaggtac 1681 taggaggaat caattgtgta aaagcctcgg ttcttacgcc ttattaccac aaagtagatt 1741 ttgagtgtat cttggataaa agaaaaaaac ctcttccgta tggaagccat aatatagcat 1801 tgggacaact accagaaatg ccctgggaat caaatatcga aatagttgga tcaaggctgc 1861 caccaggggc tgaaaggatt gctttggaat ttttggattc aaaagcactt tgtagaaata 1921 tccctcacat gaaaggaaaa tctgctatga aaaaacgaca tttggaaatt ctggggtatc 1981 gtgtaattca gatttcccag tttgaatgga actctatggc actgtcaaca aaggatgctc 2041 ggatggacta cctgagagaa tgtatatttg gagaagtcaa gtcatgtttg tacccaactt 2101 tcttgtacaa agttggcatt ataagaaagc attgcttatc aatttgttgc aacgaac