Construct: ORF ccsbBroadEn_15227
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018929.1_s300c1, BRDN0000458225
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- ALPK2 (115701)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 115701 | ALPK2 | alpha kinase 2 | NM_052947.4 | 88.7% | 12.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 98
- ORF end:
- 938
- ORF length:
- 840
- Sequence:
-
1 AAGGATTTTA TTTTGACTGA TAGTGACCTG TTCGTTGCAA CAAATTGATG AGCAATGCTT 61 TTTTATAATG CCAACTTTGT ACAAAAAAGT TGGCACCATG GCATCAAAGT TCACGGATGG 121 TGACCTGAAC AATGATGGTC CTCATGATGA AGGCTTACGC TCTAGTCAGC AAAATCCCAA 181 AGTACAGAAA TACATTAGCT TCAGCCTCCC GCTGTCTGAG GCAACTGCAC ACATTTACCC 241 AGGTGACAGT GCCGTGGCCA ACAAACAACC CAGCCCACAG CTTTCCAGTG AAGACTCTGA 301 CAGTGACTAT GAACTTTGCC CAGAGATAAC CCTAACCTAC ACCGAGGAGT TTTCAGATGA 361 TGACCTGGAG TATCTGGAAT GTTCTGATGT TATGACGGAT TACTCTAATG CAGTTTGGCA 421 AAGGAACCTG CTGGGGACTG AGCATGTTTT TTTATTAGAA AGCGATGACG AAGAGATGGA 481 ATTCGGTGAG CATTGCCTGG GTGGGTGTGA GCATTTCCTC AGTGGAATGG GTTGTGGGTC 541 TCGGGTGTCG GGTGACGCTG GGCCTATGGT TGCCACTGCT GGCTTCTGTG GTCATCACTC 601 ACAACCCCAA GAAGTTGGGG TGAGGAGCAG CAGAGTCTCC AAGCACGGTC CCTCATCCCC 661 ACAAACAGGG ATGACTCTCA TTTTGGGACC TCACCAGGAT GGAACGTCTT CAGTGACAGA 721 ACAGGGGAGA TATAAACTCC CCACTGCTCC CGAGGCTGCT GAAAATGATT ATCCAGGAAT 781 TCAAGGAGAA ACCAGAGACA GCCACCAAGC AAGAGAGGAA TTTGCCAGTG ACAATCTGCT 841 CAACATGGAT GAATCAGTAA GAGAGACAGA GATGAAGCTC CTTGTCTGGT GGAGTCAGAA 901 AAACTTCAGG GATGGAGCAg tgttgggaga cggcagctga caagagagtg gggggaaagg 961 acttatggag caagaggggt tcaaggaaat ctgccagggt gaggcagccg ggaatgaagg 1021 gaaatcccaa gaagccgaat gccaacctga gagaaagtac aacagaaggt acccttcatc 1081 tctgctctgc caaagaatct gctgagcccc cactaaccca gagtgataaa agagagactt 1141 ctcacaccac agcagcagcg actggtcgga gttcccatgc tgatgcaaga gaatgtgcta 1201 tttcaaccca ggcagagcaa gaagcaaaaa cccttcaaac ttcaacagac tcagtctcca 1261 aagaaggcaa cacaaattgc aagggagaag gcatgcaagt taatactcta tttgaaacaa 1321 gccaggttcc agactggagt gatcctcctc aggtacaagt tcaggaaaca gtcagagaga 1381 caatctcttg cagccagatg ccagctttct cagagcctgc tggggaggag tccccattca 1441 ctgggaccac aacaatttcc ttctcaaact taggaggggt ccacaaggaa aatgcatcat 1501 tagctcaaca ctcggaggtc aaaccctgta cctgtggtcc acagcatgaa gaaaaacaag 1561 acagagatgg caacatacct gacaatttca gggaagacct aaaatatgag cagagcatct 1621 cagaagccaa tgatgagact atgtccccag gtgtgttctc aaggcatctc cccaaggatg 1681 ctcgtgctga cttcagggag cctgtggctg tctctgttgc ttcccctgaa cccacagata 1741 ctgccctcac cctggaaaat gtgtgtgatg agccaaggga cagagaagca gtgtgtgcaa 1801 tggagtgttt tgaggctggt gaccaaggaa cgtgttttga taccatagat tctcttgttg 1861 ggagaccagt tgataaatat tcgcctcaag aaatttgctc tgtagatacg gaactggcag 1921 aaggtcaaaa caaagtatct gatttatgtt cttctaatga caagacactg gaagtctttt 1981 ttcagacaca agtgtctgag acttcagtgt ctacgtgcaa aagcagcaag gacggcaact 2041 cagtcatgtc ccctcttttt atcagtactt tcaccttgaa catttcacac acagctagtg 2101 aaggtgccac aggagaaaat ctagccaagg tggagaattc cacctaccca ctggcctcca 2161 cagtacatgc tggccaggag cagccaagcc ccagcaactc aggagggctt gatgaaacac 2221 agctcctttc ttctgagaac aatcctttag tgcaatttaa agaaggaggt gacaagagcc 2281 ccagtcctag tgccgcagac accacagcca caccagccag ttatagttca attgtgagtt 2341 ttccttggga gaagccaaca acattaactg ctaataatga gtgctttcaa gcgaccaggg 2401 agactgagga cacatcaact gttaccattg ccaccgaagt ccacccagcc aaataccttg 2461 ctgtgtcaat tcctgaggac aagcatgcag gtggcactga ggagaggttc cctcgtgcat 2521 cccatgaaaa ggtttcccaa tttccttccc aagtgcaggt ggatcatatt ttaagtggtg 2581 ctaccatcaa atctacaaaa gagctacttt gcagggcacc cagtgtgcca ggagtcccac 2641 accatgtcct gcagctccca gagggagagg gtttctgcag taattcccct cttcaggttg 2701 ataacctgtc tggagataag agccagactg tggacagagc agactttagg agctatgaag 2761 agaatttcca agaaagagga agtgaaacaa agcagggggt ccagcagcag agcctgtccc 2821 agcagggttc tctttctgca cctgatttcc aacaaagttt gcctacgaca tctgctgcac 2881 aagaggaaag aaacttggtg cccacggccc cctcaccagc aagctctagg gaaggagcag 2941 ggcagcgctc aggttggggg acgagggtct ccgtggtggc tgaaactgct ggggaagaag 3001 acagtcaggc tctgagcaac gttccatctc tctctgatat ccttttggaa gagtctaaag 3061 aatatagacc tggaaattgg gaggcaggca acaagctgaa gattataact ctagaggctt 3121 ccgcttctga aatctggcca ccacgacaac tgacaaattc tgagagcaag gcatcagacg 3181 gtggtctcat aattcctgac aaggtctggg ctgtacctga tagtctaaag gcagatgctg 3241 ttgtgcctga attggccccc tctgaaatag cagcattggc tcacagtcca gaggatgctg 3301 agtcagccct tgctgatagc agagaaagcc ataaaggcga agagcccacc atcagtgtac 3361 actggagaag tctttcttcc cggggtttca gccaaccgag actcctggag tcatccgtgg 3421 accctgtaga tgaaaaggag ttatctgtca cagattcact gtcagcggct tctgaaactg 3481 gagggaagga aaatgttaac aatgtgagtc aagaccagga ggaaaaacaa ctcaagatgg 3541 atcacactgc cttctttaaa aagtttctga cctgccctaa aatcctagag tcctctgtag 3601 atcccattga tgagataagt gtgatagagt acaccagggc tggaaaacca gagccctctg 3661 aaaccacacc acagggcgcc agagaagggg gtcaatcaaa tgacggaaac atgggccacg 3721 aagcggaaat ccagccggcc attttgcaag ttccatgtct ccagggaacc attctgagtg 3781 aaaatagaat cagcagaagc caagaaggca gtatgaagca ggaggcagaa caaattcaac 3841 ctgaggaggc aaaaactgcc atttggcaag tcctgcaacc cagcgaaggc ggtgaaagaa 3901 ttccaagtgg atgtagcata ggccaaatac aagaaagcag tgatgggagc ttaggggagg 3961 ctgagcaaag caaaaaggac aaagcagaat tgatttcccc cacttcacct ctttctagtt 4021 gtcttccaat aatgactcac gcttctcttg gggttgacac gcacaactcc acaggccaaa 4081 ttcatgacgt ccctgaaaat gacatagttg agcccagaaa gcgtcagtat gtgtttcctg 4141 tttcacagaa aaggggaact attgagaatg agcgtgggaa acctttgccc tcttctcctg 4201 atcttaccag gttcccttgt acttcatctc ctgaaggaaa tgtcacagac tttttgataa 4261 gccacaaaat ggaggaacct aaaatagagg tgcttcaaat tggggaaacc aaacccccaa 4321 gctcatctag ctcctcagcg aagaccttgg catttatttc aggagaacgt gagttagaga 4381 aagcccctaa gttactgcag gatccatgtc aaaagggcac cctgggctgt gcgaaaaagt 4441 ccagggagag agagaagtcc ctggaagccc gagcaggcaa atcgccaggg accctcacag 4501 cagtgacggg gtcagaggag gtcaagagga agccagaagc cccaggcagt ggacatttag 4561 ctgagggagt aaagaagaaa attttgtcca gggtggcagc actgaggctg aaactggaag 4621 aaaaggaaaa tatcagaaag aactcagcct ttcttaaaaa gatgcccaaa ctcgaaacat 4681 cattatcaca cacagaagag aaacaagacc caaaaaagcc atcttgcaaa agagaaggaa 4741 gagctccagt attactgaaa aagatccaag ctgagatgtt ccctgaacac tctggaaatg 4801 taaaattaag ctgccaattt gcagaaattc atgaagattc tactatctgc tggacaaaag 4861 attcaaagtc catagcccaa gtgcagagaa gtgcagggga caactccact gtttcctttg 4921 ccatcgtgca agccagtccg aaggaccagg gactctatta ctgctgcatc aagaacagct 4981 acggaaaagt gactgctgaa tttaacctca cagctgaagt tctcaaacag ctgtcaagtc 5041 gccaggatac taaaggatgt gaagagattg aattcagcca actcatcttc aaagaagact 5101 tcctccatga cagctacttt TGGGGCCGCC TGCGTGGTCA GATCGCCACG GAGGAGCTGC 5161 ACTNNGAGAA NGGGTTCACC GCAAAGCTTT CNGCAGCACA GTGATGCACG GCTTCATGCC 5221 TGTCTTCAAA CCTGGCCATG CCTGTGTGCT TAAGGTGCAC AATGCCATTG CCTATGGGAC 5281 CAGAAATAAT GATGAGCTCA TCCAAAGGAA CTACAAACTC GCTGCCCAGG AATGCTATGT 5341 TCAAAATACT GCCAGGTATT ATGCCAAGAT CTACGCTGCT GAAGCACAGC CTCTGGAAGG 5401 CTTTGGAGAA GTACCTGAGA TCATTCCTAT TTTTCTTATC CATCGGCCTG AGAACAATAT 5461 CCCGTATGCT ACAGTGGAGG AGGAGCTGAT TGGAGAATTT GTGAAGTATT CCATCAGGGA 5521 TGGGAAAGAA ATAAACTTCT TGAGAAGAGA ATCAGAAGCT GGTCAGAAAT GTTGCACCTT 5581 CCAGCACTGG GTGTACCAGA AAACAAGTGG CTGCCTCCTG GTGACGGACA TGCAAGGTGT 5641 AGGAATGAAG CTAACTGACG TTGGCATAGC AACGCTGGCT AAAGGGTACA AGGGATTTAA 5701 AGGCAACTGT TCCATGACCT TCATTGATCA GTTTAAAGCA CTACACCAGT GTAACAAGTA 5761 TTGCAAAATG CTGGGACTGA AATCCCTTCA AAACAACAAC CAGAAACAGA AGCAGCCGAG 5821 CATTGGGAAA AGCAAAGTTC AAACAAACTC TATGACAGTA AAGAAGGCAG GGCCTGAGAC 5881 CCCAGGCGAA AAGAAAACCT TGCCAACTTT CTTGTACAAA GTTGGCATTA TAAGAAAGCA 5941 TTGCTTATCA ATTTGTTGCA ACGAACAGGT CACTATCAGT CAAAATAAAA TCATTATTGC 6001 CATCCCAGGC TTGA