Construct: ORF ccsbBroadEn_15270
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018911.1_s300c1, BRDN0000458207
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- PRAG1 (157285)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 157285 | PRAG1 | PEAK1 related, kinase-activ... | NM_001080826.3 | 91.1% | 18.4% | (many diffs) |
| 2 | human | 157285 | PRAG1 | PEAK1 related, kinase-activ... | NM_001369759.1 | 91.1% | 18.4% | (many diffs) |
| 3 | human | 157285 | PRAG1 | PEAK1 related, kinase-activ... | NR_163138.1 | 77.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 92
- ORF end:
- 1187
- ORF length:
- 1095
- Sequence:
-
1 TTTATTTTGA CTGATAGTGA CCTGTTCGTT GCAACAAATT GATGAGCAAT GCTTTTTTAT 61 AATGCCAACT TTGTACAAAA AAGTTGGCAC CATGCACCAG ACCCTCTGCC TGAACCCCGA 121 GAGCCTGAAA ATGTCTGCGT GCAGTGACTT TGTGGAGCAC ATCTGGAAAC CCGGGTCCTG 181 CAAGAACTGC TTCTGCCTGC GGAGTGACCA CCAGCTGGTG GCCGGCCCTC CCCAGCCCAG 241 AGCGGGCAGC CTGCCCCCTC CACCGCGCCT GCCTCCCAGG CCTGAGAACT GCCGCCTGGA 301 AGATGAAGGT GTGAACAGCT CACCTTACTC CAAGCCCACA ATTGCCGTGA AGCCCACCAT 361 GATGAGCTCC GAGGCCTCTG ATGTGTGGAC AGAGGCCAAC CTGAGTGCCG AAGTCTCGCA 421 GGTCATCTGG AGACGAGCCC CTGGCAAGCT CCCCCTCCCG AAGCAGGAGG ATGCCCCCGT 481 CGTCTATCTG GGCAGCTTCC GAGGTGTACA GAAGCCTGCT GGTCCCTCTA CCTCCCCTGA 541 TGGCAATTCT CGCTGTCCCC CAGCTTACAC CATGGTCGGC CTGCACAACC TTGAGCCCCG 601 CGGCGAGAGG AACATTGCCT TCCACCCGGT GAGCTTCCCG GAGGAGAAGG CTGTGCACAA 661 AGAAAAACCC TCATTTCCTT ACCAAGACCG GCCCTCCACC CAGGAGAGCT TCCGCCAGAA 721 ACTGGCTGCC TTTGCTGGGA CCACATCTGG CTGTCACCAG GCCCTGGGCC CCTGCGGGAA 781 TCCCTGCCCT CGGAGGATGA CAGTGATCAA GTGCTCGCCC TCCGGGGACA GCGAGGGTGG 841 AGAGTACTGC TCATCCTGGA CTGCTGCCCT GGGAGCCCTG TTGCCaaggc tgcctcccag 901 actgcaggtt cccggggcag gcatggtggc agggactgct cacccacgtg ctgggagcag 961 gggaagtgtt ccgggcccgc agagcaggag aagcggggcc cgagcttccc caaggagtgc 1021 tgtagccagg gccccactgc ccacccatcc tgcctgggcc ccaagaaact gtccctcacc 1081 tcggaggctg ccatttcttc cgacggcctc tcttgtggca gcggcagcgg cagcggcgcc 1141 agtagcccct tcgtccccca cctcgagagt gattactgct ccctcatgaa ggaacctgcc 1201 ccagagaagc agcaggaccc tggctgccca ggggtgaccc ctagcagatg ccttgggctg 1261 acgggggagc cccagccccc ggcccacccc cgggaggcta cacagcctga acccatctat 1321 gctgagagca ccaagaggaa gaaggcagct ccggtgcctt ccaagtcaca ggccaagata 1381 gaacatgcag ctgctgccca gggccaaggc caggtatgca caggtaatgc ctgggcccag 1441 aaagcagcat ctggctgggg ccgggacagc ccagacccaa ctccccaggt gtcagccacc 1501 atcacagtca tggcggccca cccggaagag gaccatcgga cgatctacct gagcagccct 1561 gactctgcag tgggggtgca gtggccacga gggcctgtga gccagaactc cgaggtaggt 1621 gaagaggaga cttcggctgg gcaggggctg agctccaggg aaagccatgc tcacagtgcc 1681 agcgagagca agcccaagga gaggcccgcc attcccccca agttgtccaa gagtagccct 1741 gtagggtccc cggtgtcacc gtctgctgga gggcccccag tgtcaccgct ggctgacctt 1801 agtgatggga gctctggcgg cagcagcatt gggccccagc ctccatccca aggtcctgct 1861 gaccccgctc cttcctgccg gaccaacggt gtcgctatca gtgacccatc caggtgtccc 1921 cagcctgccg cctcgtcagc ctcggaacag aggcggccca ggttccaggc aggcacctgg 1981 agtcgtcagt gccggataga ggaagaagag gaggtggagc aggaattgct gagtcacagc 2041 tggggaagag agaccaaaaa tggccccacg gaccattcaa actccacgac ctggcaccgt 2101 ctccacccca cagatggctc ctctgggcag aacagcaaag ttgggaccgg gatgagcaaa 2161 tccgcctctt ttgcctttga gttccccaag gacagaagtg ggattgagac attctcacct 2221 cctcctccgc ctccaaagtc gcggcacctt ctaaaaatga acaagagcag ctctgatttg 2281 gaaaaagtga gccagggctc tgcagaaagc ctcagcccat ccttcagggg tgtccacgtc 2341 agcttcacca ccggctccac ggacagcctg gcctcagact ctaggacctg cagcgatgga 2401 ggtccctcgt ctgagctggc tcactcgccc accaacagcg ggaagaagct ctttgctccc 2461 gttccgtttc cttcaggctc cactgaggac gtgtccccca gtggccccca gcagccccct 2521 ccactccccc agaaaaagat agtgagccgg gcagcctctt caccggatgg cttcttctgg 2581 acccaaggct cccccaagcc cggaacagca agccccaagc tgaacctaag ccactcggaa 2641 accaacgtcc acgacgaatc tcactttagc tattcgttga gccccgggaa ccgccaccat 2701 cctgtcttct cctcttccga tcctctggag aaagctttca aaggcagtgg ccactggctt 2761 ccggcagcag ggctggcggg caacagaggc ggctgcggga gccctggcct ccagtgcaaa 2821 ggggccccct ccgcctcatc ctcccagctg agcgtgtcca gtcaagcctc caccgggagc 2881 acccagcttc agctgcacgg tctcctgagc aacatcagca gcaaggaggg cacctatgcc 2941 aagctggggg gactctacac ccagtccctg gcccgccttg tagccaaatg tgaggacctc 3001 ttcatgggcg gccagaaaaa ggagctccac ttcaatgaga ataactggtc gctcttcaag 3061 ctgacttgta acaagccctg ctgtgactcg ggggatgcca tttattactg tgccacctgc 3121 tctgaggacc ccggcagcac ctatgctgtg aaaatctgca aagcccctga gcccaaaaca 3181 gtctcctact GCAACCCGTT CGGGCCCGTG GCATTTTACA TCCCAGCAGG ATTGCGGCCA 3241 GTTTTGTGGC TTGGGTTCCG TCCAGCCTGT CTAAGTTCCC CCGGACGGGC CTCCCTGGTC 3301 CTCAGCCCGC CCCGCCTGCT GGTGGCACTT TCAGCCCCGC AACCGGCCCC GCCCTCCCCG 3361 GAAGGGCCCC GGAGAAAGCA GCTGCCCGGA CTCATCTTCA GCAACTTTTT GAAGGCCAAG 3421 CAGAAGCCGG GCGGCACCCC AACCCTGCAG CAGAAGAAGA GCCAGGCCCG GCTGGCCCCC 3481 GAGATCGTGT CTGCTTCCCA GTACCGCAAG TTCGATGAGT TCCAGACAGG CATCCTCATC 3541 TACGAGCTGC TGCACCAACC CAACCCGTTC GAGGTGCGCG CCCAGCTGCG GGAGAGAGAC 3601 TACCGGCAGG AGGACCTGCC GCCGCTGCCC GCGCTGTCCC TCTACTCACC CGGCCTGCAG 3661 CAGCTGGCAC ATCTGCTACT GGAGGCCGAC CCCATCAAGC GTATCCGCAT CGGCGAGGCC 3721 AAGCGCGTGC TGCAGTGCCT GCTGTGGGGG CCTCGGCGCG AGCTGGTGCA GCAGCCGGGC 3781 ACCTCGGAGG AGGCGCTGTG CGGCACGCTG CACAACTGGA TCGACATGAA GCGGGCCCTG 3841 ATGATGATGA AGTTTGCGGA GAAGGCGGTG GATCGCAGGC GGGGCGTGGA GCTGGAGGAC 3901 TGGCTTTGCT GCCAGTACCT GGCGTCTGCG GAGCCCGGGG CCCTCTTACA GTCGCTGAAG 3961 CTCCTGCAGC TTCTGTTGCC AACTTTCTTG TACAAAGTTG GCATTATAAG AAAGCATTGC 4021 TTATCAATTT GTTGCAACGA ACAGGTCACT ATCAGTCAAA ATAAAATCAT TATTGCCATC 4081 CAGCCTGAAT CCCCC