Transcript: Human NR_163138.1

Homo sapiens PEAK1 related, kinase-activating pseudokinase 1 (PRAG1), transcript variant 3, non-coding RNA.

Source:
NCBI, updated 2019-04-17
Taxon:
Homo sapiens (human)
Gene:
PRAG1 (157285)
Length:
4936
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
NR_163138.1
NBCI Gene record:
PRAG1 (157285)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NR_163138.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000199392 CCACGACGAATCTCACTTTAG pLKO.1 2865 3UTR 100% 10.800 15.120 N PRAG1 n/a
2 TRCN0000037424 GCACAACTGGATCGACATGAA pLKO.1 4350 3UTR 100% 4.950 6.930 N PRAG1 n/a
3 TRCN0000037428 GTACCGCAAGTTCGATGAGTT pLKO.1 4041 3UTR 100% 0.000 0.000 N PRAG1 n/a
4 TRCN0000037425 CGGAGGATGACAGTGATCAAA pLKO.1 907 3UTR 100% 5.625 4.500 N PRAG1 n/a
5 TRCN0000298762 CGGAGGATGACAGTGATCAAA pLKO_005 907 3UTR 100% 5.625 4.500 N PRAG1 n/a
6 TRCN0000200015 CCAGGTATGCACAGGTAATGC pLKO.1 1535 3UTR 100% 4.950 3.960 N PRAG1 n/a
7 TRCN0000199835 GAGTCGTCAGTGCCGGATAGA pLKO.1 2105 3UTR 100% 1.650 1.320 N PRAG1 n/a
8 TRCN0000307996 GAGTCGTCAGTGCCGGATAGA pLKO_005 2105 3UTR 100% 1.650 1.320 N PRAG1 n/a
9 TRCN0000381719 ACAGCGGGAAGAAGCTCTTTG pLKO_005 2651 3UTR 100% 10.800 7.560 N PRAG1 n/a
10 TRCN0000310168 GCCAGGTATGCACAGGTAATG pLKO_005 1534 3UTR 100% 10.800 7.560 N PRAG1 n/a
11 TRCN0000295928 TCGTCTCCTCTCTGCACTAAG pLKO_005 4717 3UTR 100% 10.800 7.560 N PRAG1 n/a
12 TRCN0000199343 CGGCTCATCATCAGCAACTTT pLKO.1 3928 3UTR 100% 5.625 3.938 N PRAG1 n/a
13 TRCN0000037427 CCTGGAAGATGAAGGTGTGAA pLKO.1 410 3UTR 100% 4.950 3.465 N PRAG1 n/a
14 TRCN0000037426 CGCTCTTCAAGCTGACTTGTA pLKO.1 3266 3UTR 100% 4.950 3.465 N PRAG1 n/a
15 TRCN0000199792 GATGAGCAAATCCGCCTCTTT pLKO.1 2276 3UTR 100% 4.950 3.465 N PRAG1 n/a
16 TRCN0000199666 GTCTCCTCTCTGCACTAAGTC pLKO.1 4719 3UTR 100% 4.950 3.465 N PRAG1 n/a
17 TRCN0000199870 GAGTGATTACTGCTCCCTCAT pLKO.1 1292 3UTR 100% 4.050 2.835 N PRAG1 n/a
18 TRCN0000382470 AGCTCCACTTCAATGAGAATA pLKO_005 3239 3UTR 100% 13.200 7.920 N PRAG1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NR_163138.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_15270 pDONR223 41% 77.9% None (many diffs) n/a
2 ccsbBroad304_15270 pLX_304 0% 77.9% V5 (not translated due to prior stop codon) (many diffs) n/a
3 TRCN0000471519 CGCCCTGGAGCCCAAGCTTATCAA pLX_317 10.1% 77.9% V5 (not translated due to prior stop codon) (many diffs) n/a
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