Construct: ORF ccsbBroadEn_15291
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018824.1_s300c1, BRDN0000458120
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- PRPS1L1 (221823)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 221823 | PRPS1L1 | phosphoribosyl pyrophosphat... | NM_175886.3 | 99.5% | 1.8% | 187A>N;760_762delTCTinsNNN |
| 2 | human | 5631 | PRPS1 | phosphoribosyl pyrophosphat... | NM_002764.4 | 91.9% | 1.5% | (many diffs) |
| 3 | human | 5631 | PRPS1 | phosphoribosyl pyrophosphat... | NM_001204402.1 | 31.7% | 4.3% | (many diffs) |
| 4 | mouse | 328099 | Prps1l3 | phosphoribosyl pyrophosphat... | NM_001037746.3 | 89.6% | 1.5% | (many diffs) |
| 5 | mouse | 19139 | Prps1 | phosphoribosyl pyrophosphat... | NM_021463.4 | 89.3% | 1.5% | (many diffs) |
| 6 | mouse | 75456 | Prps1l1 | phosphoribosyl pyrophosphat... | NM_029294.2 | 87% | 1.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 215
- ORF end:
- 281
- ORF length:
- 66
- Sequence:
-
1 CTGATAGTGA CCTGTTCGTT GCAACAAATT GATGAGCAAT GCTTTTTTAT AATGCCAACT 61 TTGTACAAAA AAGTTGGCAC CACGCCGAAT ATCAAAATCT TCAGCGGCAG CTCCCACCAG 121 GACTTATCCC AGAAAATTGC TGACCGCCTG GGCCTGGAGC TAGGCAAGGT GGTGACTAAG 181 AAATTCAGCA ACCAGGAGAC CTGCGTGGAA ATTGATGAGA GTGTGCGTGG AGAGGATGTC 241 TACATCGTTC AGAGTGGTTG TGGCGAANTC AACGACAGTC TAATGGAGCT TTTGATCATG 301 ATTAATGCCT GCAAGATTGC TTCAGCTAGC CGAGTTACTG CAGTCATCCC ATGCTTCCCT 361 TATGCCCGAC AGGATAAGAA GGATAAGAGC CGGTCCCCAA TCTCTGCCAA GCTTGTTGCA 421 AATATGCTCT CTATAGCAGG TGCGGATCAT ATCATCACCA TGGACCTACA TGCTTCTCAA 481 ATTCAGGGCT TTTTTGATAT CCCAGTAGAC AACTTGTATG CAGAGCCAAC TGTCCTGAAG 541 TGGATAAGGG AGAATATCCC TGAGTGGAAG AACTGCATTA TTGTCTCGCC AGATGCTGGT 601 GGAGCTAAAA GAGTGACCTC CATTGCAGAC CAGTTGAATG TGGACTTTGC TTTGATTCAT 661 AAAGAACGGA AGAAGGCCAA TGAAGTGGAC TGCATAGTGC TAGTGGGAGA TGTGAATGAT 721 CGTGTGGCTA TCCTTGTAGA TGACATGGCA GACACTTGTG TTACAATCTG CCTCGCAGCT 781 GACAAACTTC TCTCAGCTGG AGCAACCAGA GTTTATGCTA TCTTGACTCA TGGAATCTTT 841 NNNGGCCCAG CCATTTCTCG CATCAACACT GCATGCTTTG AAGCAGTGGT AGTCACCAAT 901 ACCATACCTC AAGATGAGAA GATGAAGCAT TGCTCCAAAA TACGAGTAAT TGACATCTCC 961 ATGATCCTTG CAGAAGCCAT AAGGAGAACT CATAATGGGG AATCTGTTTC CTACCTGTTC 1021 AGCCATGTTC CTTTATTGCC AACTTTCTTG TACAAAGTTG GCATTATAAG AAAGCATTGC 1081 TTATCAATTT GTTGCAACGA ACAGGTCACT ATCAGTCAAA ATAAAATCAT TATTGCCATC 1141 CAGCGATCAA TA