Construct: ORF ccsbBroadEn_15298

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF018854.1_s300c1, BRDN0000458150
DNA Barcode:
None
Epitope Tag:
None
Notes:
Early stop codon detected

Originally Annotated References:

Gene:
NEK8 (284086)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 284086 NEK8 NIMA related kinase 8 NM_178170.3 79% 75.5% (many diffs)
2 human 102723842 LOC102723842 uncharacterized LOC102723842 XR_428160.3 8.2% 0_1ins1996;176G>A;195_346del
3 mouse 140859 Nek8 NIMA (never in mitosis gene... XM_006532183.3 70.8% 71.5% (many diffs)
4 mouse 140859 Nek8 NIMA (never in mitosis gene... XM_006532184.3 70.1% 72% (many diffs)
5 mouse 140859 Nek8 NIMA (never in mitosis gene... NM_080849.3 70.1% 71.9% (many diffs)
6 mouse 140859 Nek8 NIMA (never in mitosis gene... XM_006532185.3 65.6% 65.2% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1689
ORF length:
1620
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaactttg tacaaaaaag
61ttggcaccat ggagaagtac gagcggatcc gagtggtggg gagaggtgcc ttcgggattg
121tgcacctgtg cctgcgaaag gctgaccaga agctggtgat catcaagcag attccagtgg
181aacagatgac caaggaagag cggcaggcag cccagaatga gtgccaggtc ctcaagctgc
241tcaaccaccc caatgtcatt gagtactacg agaacttcct ggaagacaaa gcccttatga
301tcgccatgga atatgcacca ggcggcactc tggctgagtt catccaaaag cgctgtaatt
361ccctgctgga ggaggagacc atcctgcact tcttcgtgca gatcctgctt gcactgcatc
421atgtgcacac ccacctcatc ctgcaccgag acctcaagac ccagaacatc ctgcttgaca
481aacaccgcat ggtcgtcaag atcggtgatt tcggcatctc caagatcctt agcagcaaga
541gcaaggccta cacggtggtg ggtaccccat gctatatctc ccctgagctg tgtgagggca
601agccctacaa ccagaagagt gacatctggg ccctgggctg tgtcctctac gagctggcca
661gcctcaagag ggctttcgag gctgcgaact tgccagcact ggtgctgaag atcatgagtg
721gcacctttgc acctatctct gaccggtaca gccctgagct tcgccagctg gtcctgagtc
781tactcagcct ggagcctgcc cagcggccac cactcagcca catcatggca cagcccctct
841gcatccgtgc cctcctcaac ctccacaccg acgtgggcag tgtccgcatg cggagggcag
901agaagtccgt ggcccccagc aacacaggga gcaggaccac cagtgtccgc tgcagaggta
961tcccccgggg acctgtgagg ccagccatcc caccaccact gtcgtcagtg tatgcctggg
1021gtggtgggct gggcaccccc ctgcggctgc caatgctcaa cacagaggtg gtccaggtgg
1081cagctgggcg cacgcagaaa gccggcgtca cgcgctctgg gcgtctcatc ctgtgggagg
1141ccccacccct aggtgcaggc ggaggcagtc tccttcctgg ggcagtggag cagccacagc
1201cccagttcat ctcgcgtttc ctggagggcc agtcgggtgt gaccatcaag cacgtggcct
1261gtggggactt cttcactgcc tgcctgactg acagaggcat catcatgaca ttcggcagcg
1321gcagcaatgg gtgcctaggc catggcagcc tcactgacat cagccagccc accattgtgg
1381aggctttgct gggctatgaa atggtgcagg tggcctgtgg ggcctctcac gtgctggccc
1441tgtccactga gcgagaacta tttgcctggg gccgtggaga cagcggcaga ctggggctag
1501gcaccaggga gtcccacagc tgcccccagc aggtgcccat gcccccagga caggaagctc
1561agcgagttgt atgtggtatc gattcctcca tgatcctcac tgtgcctggc caagccctag
1621cctgtgggag AAACATCCCA GTCCCGACGA TGCAACACGA CTGGCCAGTA TTAAGCCTGG
1681GCCCCAAATA ATGATTTTAT TTTGACTGAT AGTGACCTGT TCGTTGCAAC AAATTGATGA
1741GCAATGCTTT TTTATAATGC CAACTTTGTA CTATGATTTT ATTTTGACTG ATANNNANNT
1801NNNCNNCATG GCTTTTTGCC CGCCGTGGCT TNNAACAAAT TGATGAGCAA TGCTTTTTTA
1861TAATGCCAAC TTTGTACAAA AAAGTTGGCA CCATGGCTTT TTGCCCGCCG TGGCTTTTTC
1921CCCCCGCCGC CGCGGCTTTT TGTGGGTTTG TTTTTTTTTG CCGCCGCGGC TTTTTGCCCC
1981GCCGCCGCCG CTTTTTGCCG CCGCGGCTTT TTACCCCCGC CGCCGAGGAT TTTTGCCCCC
2041GCCGCCGCGG CTCTGAGGGC GGGAGCGGCA CACTCGGCTG CCAGCTCCAC TGGCGTTCTG
2101GCAAGGGCAG CGCGGAGGGG CGCTCCTGGT CCAGCTCTCC CTGCTCGGGA GTGTATGAAT
2161GCTTTGTGTT CTGACAAAGA GACTGTAGCT CCCATGTCCT ACCTGCTTGG TTGCATTGCC
2221AGTGCCCACG TCATTGCTTA TTTAAAATCT ACAACTGCTT GCCAACTTTC TTGTACAAAG
2281TTGGCATTAT AAGAAAGCAT TGCTTATCAA TTNGTTGCAA CGAACAGGTC ACTATCAGTC
2341AAAATAAAAT CATTATTTGC CATCCNNNTG ATNNNNNGGA TGTTGAGTCG CATTACATGG
2401TCATAGCTGT TTCCTGGCAG CTCTGGCCCG TGTCTCAAAT CTCTGATGTT ACAGGACATG
2461GCAAATATCA TGTGTAAGCT GGTGATATAG AACGCATTGC TCATGAATAT GTGCAACAAA
2521CAGAGTAAAA TATCAGTCCA ATACGGCGTA ATTTGGCATC CAGCTGATTC CTCTGTTGTT
2581GAATCGTAGT AGACTGGTCA AAAAAAATTA CCTGGATGTC TGGCCCGGTC ATTATGCTGA
2641GCTAATAATT ATAAAACAAG CCTGGGTCTC GC

Download FASTA (ORF) (Full)

GPP Web Portal Terms of Service

Effective Date: December 8, 2025
By using this site, you agree to our terms and conditions below.

Overview of Terms

The data made available on this website were generated for research purposes and are not intended for clinical or commercial uses. Commercial use (or other use for profit-making purposes) of the GPP Web Portal and its tools, is not permitted under these terms and may require a separate license agreement from Broad or its contributors. For more information, please contact partnering@broadinstitute.org.

The original data may be subject to rights claimed by third parties, including but not limited to, patent, copyright, other intellectual property rights, biodiversity-related access and benefit-sharing rights. It is the responsibility of users of Broad Institute services to ensure that their use of the data does not infringe any of the rights of such third parties.

Any questions or comments concerning these Terms of Use can be addressed to: legal@broadinstitute.org.

By accessing and viewing this GPP Web Portal, you agree to the following terms and conditions:

Attribution

You agree to acknowledge the Broad Institute (e.g., in publications, services or products) for any of your use of its online services, databases or software in accordance with good scientific practice. You agree to use the acknowledgment wording provided for the relevant tools as indicated on the FAQ for each tool.

Updating the Terms of Use

We reserve the right to update these Terms of Use at any time. When alterations are inevitable, we will attempt to give reasonable notice of any changes by placing a notice on our website, but you may wish to check each time you use the website. The date of the most recent revision will appear on this, the "GPP Web Portal Terms of Use" page. If you do not agree to these changes, please do not continue to use our online services. We will also make available an archived copy of the previous Terms of Use for comparison.

Indemnification and Disclaimer of Warranties

You are using this GPP Web Portal at your own risk, and you hereby agree to hold Broad and its contributors and their trustees, directors, officers, employees, and affiliated investigators harmless for any third party claims which may arise from your use of the GPP Web Portal, the tools available therein, or any portion thereof. Further, you agree to indemnify Broad, its contributors, and its and their trustees, directors, officers, employees, affiliated investigators, students, and affiliates for any loss, costs, claims, damages, or other liabilities arising from any unpermitted commercial or profit-making use you make of the GPP Web Portal. The GPP Web Portal is a research tool and is provided "as is". Broad does not represent that the GPP Web Portal is free of errors or bugs or suitable for any particular tasks.

ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE, NONINFRINGEMENT, OR THE ABSENCE OF LATENT OR OTHER DEFECTS ARE DISCLAIMED. IN NO EVENT SHALL BROAD OR ITS CONTRIBUTORS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES HOWEVER CAUSED AND ON ANY THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE OF THE GPP WEB PORTAL, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH DAMAGE.

Governing Law

The terms and conditions herein shall be construed, governed, interpreted, and applied in accordance with the internal laws of the Commonwealth of Massachusetts, U.S.A. Furthermore, by accessing, downloading, or using the Database, You consent to the personal jurisdiction of, and venue in, the state and federal courts within Massachusetts with respect to Your download or use of the Database.