Construct: ORF ccsbBroadEn_15502

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF009324.1_s300c1, BRDN0000394907
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
MTRR (4552)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 4552 MTRR 5-methyltetrahydrofolate-ho... NM_001364440.2 100% 100%
2 human 4552 MTRR 5-methyltetrahydrofolate-ho... NM_001364441.2 100% 100%
3 human 4552 MTRR 5-methyltetrahydrofolate-ho... NM_001364442.2 100% 100%
4 human 4552 MTRR 5-methyltetrahydrofolate-ho... NM_002454.3 100% 100%
5 human 4552 MTRR 5-methyltetrahydrofolate-ho... NM_024010.4 100% 100%
6 human 4552 MTRR 5-methyltetrahydrofolate-ho... XM_024446064.1 100% 100%
7 human 4552 MTRR 5-methyltetrahydrofolate-ho... XM_024446063.1 97.8% 97.8% 1_45del
8 human 4552 MTRR 5-methyltetrahydrofolate-ho... NR_157168.2 64.6% 1_67del;335_336insGGTATGGGTTACTG;2148_3205del
9 human 4552 MTRR 5-methyltetrahydrofolate-ho... NR_157173.2 64.5% 1_67del;1212_1234del;2185_3242del
10 human 4552 MTRR 5-methyltetrahydrofolate-ho... NR_157177.2 64.3% 1_93del;493_494insGTTTA;2183_3240del
11 human 4552 MTRR 5-methyltetrahydrofolate-ho... NR_134480.2 64% 1_93del;361_362insGGTATGGGTTACTG;2174_3231del
12 human 4552 MTRR 5-methyltetrahydrofolate-ho... NR_157178.2 64% 1_93del;1238_1260del;2211_3268del
13 human 4552 MTRR 5-methyltetrahydrofolate-ho... NR_134481.2 61.7% 1_93del;1146_1147ins89;2099_3156del
14 human 4552 MTRR 5-methyltetrahydrofolate-ho... NR_157175.2 61.6% 1_93del;1420_1571del;2340_3397del
15 human 4552 MTRR 5-methyltetrahydrofolate-ho... XR_001742076.1 61.4% 1_103del;1007_1146del;2338_3406del
16 human 4552 MTRR 5-methyltetrahydrofolate-ho... XR_001742074.1 61.3% 1_103del;1156_1157ins89;2109_3177del
17 human 4552 MTRR 5-methyltetrahydrofolate-ho... XR_001742072.1 61.2% 1_103del;1430_1581del;2350_3418del
18 human 4552 MTRR 5-methyltetrahydrofolate-ho... XR_001742077.1 61% (many diffs)
19 human 4552 MTRR 5-methyltetrahydrofolate-ho... XR_001742071.1 60.8% (many diffs)
20 human 4552 MTRR 5-methyltetrahydrofolate-ho... NR_157169.2 60.2% 1_67del;195_196ins154;2008_3065del
21 human 4552 MTRR 5-methyltetrahydrofolate-ho... NR_157171.2 59.8% (many diffs)
22 human 4552 MTRR 5-methyltetrahydrofolate-ho... NR_134482.2 59.7% 1_93del;221_222ins154;2034_3091del
23 human 4552 MTRR 5-methyltetrahydrofolate-ho... NR_157176.2 58.8% (many diffs)
24 human 4552 MTRR 5-methyltetrahydrofolate-ho... XR_001742075.1 58.6% (many diffs)
25 human 4552 MTRR 5-methyltetrahydrofolate-ho... NR_157170.2 57.3% (many diffs)
26 human 4552 MTRR 5-methyltetrahydrofolate-ho... NR_157174.2 57.1% (many diffs)
27 human 4552 MTRR 5-methyltetrahydrofolate-ho... NR_157172.2 57% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2163
ORF length:
2094
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaactttg tacaaaaaag
61ttggcaccat gaggaggttt ctgttactat atgctacaca gcagggacag gcaaaggcca
121tcgcagaaga aatatgtgag caagctgtgg tacatggatt ttctgcagat cttcactgta
181ttagtgaatc cgataagtat gacctaaaaa ccgaaacagc tcctcttgtt gttgtggttt
241ctaccacggg caccggagac ccacccgaca cagcccgcaa gtttgttaag gaaatacaga
301accaaacact gccggttgat ttctttgctc acctgcggta tgggttactg ggtctcggtg
361attcagaata cacctacttt tgcaatgggg ggaagataat tgataaacga cttcaagagc
421ttggagcccg gcatttctat gacactggac atgcagatga ctgtgtaggt ttagaacttg
481tggttgagcc gtggattgct ggactctggc cagccctcag aaagcatttt aggtcaagca
541gaggacaaga ggagataagt ggcgcactcc cggtggcatc acctgcatcc tcgaggacag
601accttgtgaa gtcagagctg ctacacattg aatctcaagt cgagcttctg agattcgatg
661attcaggaag aaaggattct gaggttttga agcaaaatgc agtgaacagc aaccaatcca
721atgttgtaat tgaagacttt gagtcctcac ttacccgttc ggtaccccca ctctcacaag
781cctctctgaa tattcctggt ttacccccag aatatttaca ggtacatctg caggagtctc
841ttggccagga ggaaagccaa gtatctgtga cttcagcaga tccagttttt caagtgccaa
901tttcaaaggc agttcaactt actacgaatg atgccataaa aaccactctg ctggtagaat
961tggacatttc aaatacagac ttttcctatc agcctggaga tgccttcagc gtgatctgcc
1021ctaacagtga ttctgaggta caaagcctac tccaaagact gcagcttgaa gataaaagag
1081agcactgcgt ccttttgaaa ataaaggcag acacaaagaa gaaaggagct accttacccc
1141agcatatacc tgcgggatgt tctctccagt tcatttttac ctggtgtctt gaaatccgag
1201caattcctaa aaaggcattt ttgcgagccc ttgtggacta taccagtgac agtgctgaaa
1261agcgcaggct acaggagctg tgcagtaaac aaggggcagc cgattatagc cgctttgtac
1321gagatgcctg tgcctgcttg ttggatctcc tcctcgcttt cccttcttgc cagccaccac
1381tcagtctcct gctcgaacat cttcctaaac ttcaacccag accatattcg tgtgcaagct
1441caagtttatt tcacccagga aagctccatt ttgtcttcaa cattgtggaa tttctgtcta
1501ctgccacaac agaggttctg cggaagggag tatgtacagg ctggctggcc ttgttggttg
1561cttcagttct tcagccaaac atacatgcat cccatgaaga cagcgggaaa gccctggctc
1621ctaagatatc catctctcct cgaacaacaa attctttcca cttaccagat gacccctcaa
1681tccccatcat aatggtgggt ccaggaaccg gcatagcccc gtttattggg ttcctacaac
1741atagagagaa actccaagaa caacacccag atggaaattt tggagcaatg tggttgtttt
1801ttggctgcag gcataaggat agggattatc tattcagaaa agagctcaga catttcctta
1861agcatgggat cttaactcat ctaaaggttt ccttctcaag agatgctcct gttggggagg
1921aggaagcccc agcaaagtat gtgcaagaca acatccagct tcatggccag caggtggcga
1981gaatcctcct ccaggagaac ggccatattt atgtgtgtgg agatgcaaag aatatggcca
2041aggatgtaca tgatgccctt gtgcaaataa taagcaaaga ggttggagtt gaaaaactag
2101aagcaatgaa aaccctggcc actttaaaag aagaaaaacg ctaccttcag gatatttggt
2161catnnccaac tttcttgtac aaagttggca ttataagaaa gcattgctta tcaatttgtt
2221gcaacgaac

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