Construct: ORF ccsbBroadEn_15522
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011582.1_s300c1, BRDN0000385135
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PEX10 (5192)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5192 | PEX10 | peroxisomal biogenesis fact... | NM_002617.4 | 100% | 100% | |
| 2 | human | 5192 | PEX10 | peroxisomal biogenesis fact... | NM_001374425.1 | 94.4% | 94.4% | 601_657del |
| 3 | human | 5192 | PEX10 | peroxisomal biogenesis fact... | NM_153818.2 | 94.2% | 94.2% | 601_660del |
| 4 | human | 5192 | PEX10 | peroxisomal biogenesis fact... | XM_011541576.2 | 61.3% | 61% | 597_598insTACCTCCGTGTC;600_601ins366 |
| 5 | human | 5192 | PEX10 | peroxisomal biogenesis fact... | NM_001374427.1 | 55.8% | 55.8% | 0_1ins432 |
| 6 | human | 5192 | PEX10 | peroxisomal biogenesis fact... | NM_001374426.1 | 52.7% | 52.7% | 0_1ins432;169_225del |
| 7 | human | 5192 | PEX10 | peroxisomal biogenesis fact... | XR_946666.2 | 48.7% | 1_69del;663_664insACGT;1044_1993del | |
| 8 | human | 5192 | PEX10 | peroxisomal biogenesis fact... | NR_164636.1 | 32.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1044
- ORF length:
- 978
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaactttg tacaaaaaag 61 ttggcatggc cccggccgcc gccagccccc cggaggtgat ccgcgcggcg cagaaggacg 121 agtactaccg cggtgggctg cggagcgcgg cgggcggcgc cctgcacagc ctggcgggtg 181 cgaggaagtg gctggagtgg aggaaggagg ttgagctgct ctcagatgtg gcctactttg 241 gcctcaccac acttgcaggc taccagaccc tgggggagga gtacgtcagc atcatccagg 301 tggacccatc gcggatacat gtgccctcct cgctgcgccg tggcgtgctg gtgacactgc 361 atgccgtcct gccctacctg ctggacaagg ccctgctccc cctggagcag gagctgcagg 421 ctgaccccga cagtgggcga cccttgcagg ggagcctggg gccaggtggg cgtggctgct 481 caggggcgcg gcgctggatg cgtcaccaca cggccaccct gactgagcag cagaggaggg 541 cgctgctgcg ggcggtcttc gtcctcagac agggcctcgc ctgcctccag cggctacatg 601 ttgcctggtt ttacatccac ggtgtcttct accacctggc caagaggctc acggggatca 661 cgtacctccg tgtccgcagc ctgcccggag aggacctgag ggcccgtgtt agctacaggc 721 tgctgggggt catctcactg ctgcacctgg tgctgtccat ggggctgcag ctgtacggtt 781 tcaggcagcg gcagcgagcc aggaaggagt ggaggctgca ccgcggcctg tctcaccgca 841 gggcctcctt ggaggagaga gccgtttcca gaaaccccct gtgcaccctg tgcctggagg 901 agcgcaggca cccaacagcc acgccctgcg gccacctgtt ctgctgggag tgcatcaccg 961 cgtggtgcag cagcaaggcg gagtgtcccc tctgccggga gaagttccct ccccagaagc 1021 tcatctacct tcggcactac cgctnnccaa ctttcttgta caaagttggc attataagaa 1081 agcattgctt atcaatttgt tgcaacgaac