Construct: ORF ccsbBroadEn_15692
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003239.1_s300c1, BRDN0000385126
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SCAMP3 (10067)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10067 | SCAMP3 | secretory carrier membrane ... | NM_052837.3 | 100% | 100% | |
| 2 | human | 10067 | SCAMP3 | secretory carrier membrane ... | NM_005698.4 | 92.5% | 92.5% | 65_142del |
| 3 | mouse | 24045 | Scamp3 | secretory carrier membrane ... | NM_011886.3 | 80.8% | 83.3% | (many diffs) |
| 4 | mouse | 24045 | Scamp3 | secretory carrier membrane ... | NM_001309909.1 | 80.6% | 83.1% | (many diffs) |
| 5 | mouse | 24045 | Scamp3 | secretory carrier membrane ... | NM_001309910.1 | 71.7% | 73.9% | (many diffs) |
| 6 | mouse | 105943584 | Gm45927 | predicted gene, 45927 | NM_001310017.1 | 52.2% | 52% | (many diffs) |
| 7 | mouse | 105943584 | Gm45927 | predicted gene, 45927 | NM_001310016.1 | 36.7% | 36.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1029
- ORF length:
- 963
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaactttg tacaaaaaag 61 ttggcatggc tcagagcaga gacggcggaa acccgttcgc cgagcccagc gagcttgaca 121 acccctttca gccaccacca gcctatgagc ctccagcccc tgccccattg cctccaccct 181 cagctccctc cttgcagccc tcgagaaagc tcagccccac agaacctaag aactatggct 241 catacagcac tcaggcctca gctgcagcag ccacagctga gctgctgaag aaacaggagg 301 agctcaaccg gaaggcagag gagttggacc gaagggagcg agagctgcag catgctgccc 361 tggggggcac agctactcga cagaacaatt ggccccctct accttctttt tgtccagttc 421 agccctgctt tttccaggac atctccatgg agatccccca agaatttcag aagactgtat 481 ccaccatgta ctacctctgg atgtgcagca cgctggctct tctcctgaac ttcctcgcct 541 gcctggccag cttctgtgtg gaaaccaaca atggcgcagg ctttgggctt tctatcctct 601 gggtcctcct tttcactccc tgctcctttg tctgctggta ccgccccatg tataaggctt 661 tccggagtga cagttcattc aatttcttcg ttttcttctt cattttcttc gtccaggatg 721 tgctctttgt cctccaggcc attggtatcc caggttgggg attcagtggc tggatctctg 781 ctctggtggt gccgaagggc aacacagcag tatccgtgct catgctgctg gtcgccctgc 841 tcttcactgg cattgctgtg ctaggaattg tcatgctgaa acggatccac tccttatacc 901 gccgcacagg tgccagcttt cagaaggccc agcaagaatt tgctgctggt gtcttctcca 961 accctgcggt gcgaaccgca gctgccaatg cagccgctgg ggctgctgaa aatgccttcc 1021 gggccccgtn nccaactttc ttgtacaaag ttggcattat aagaaagcat tgcttatcaa 1081 tttgttgcaa cgaac