Construct: ORF ccsbBroadEn_16016

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF007344.1_s300c1, BRDN0000395433
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
LAS1L (81887)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 81887 LAS1L LAS1 like ribosome biogenes... NM_001170649.1 100% 100%
2 human 81887 LAS1L LAS1 like ribosome biogenes... XM_017029877.2 99.8% 99.8% 1874_1875insAGA
3 human 81887 LAS1L LAS1 like ribosome biogenes... NM_031206.4 97.6% 97.5% 1043_1093del
4 human 81887 LAS1L LAS1 like ribosome biogenes... XM_005262301.2 97.5% 97.4% 1043_1093del;1925_1926insAGA
5 human 81887 LAS1L LAS1 like ribosome biogenes... NM_001170650.1 94.1% 94.1% 233_234ins126
6 human 81887 LAS1L LAS1 like ribosome biogenes... XM_011531045.2 91.9% 91.8% 233_234ins126;917_967del
7 human 81887 LAS1L LAS1 like ribosome biogenes... XR_001755730.2 85.1% 1_63del;1461_1576del;2331_2526del
8 human 81887 LAS1L LAS1 like ribosome biogenes... XR_244504.2 83.4% (many diffs)
9 human 81887 LAS1L LAS1 like ribosome biogenes... XR_430522.2 83.3% (many diffs)
10 human 81887 LAS1L LAS1 like ribosome biogenes... XR_938412.2 72% (many diffs)
11 human 81887 LAS1L LAS1 like ribosome biogenes... XM_005262304.2 67.9% 60.5% (many diffs)
12 human 81887 LAS1L LAS1 like ribosome biogenes... XM_017029878.2 65.5% 65.1% (many diffs)
13 human 81887 LAS1L LAS1 like ribosome biogenes... XM_011531046.2 64.3% 64.3% (many diffs)
14 human 81887 LAS1L LAS1 like ribosome biogenes... XM_005262306.4 64% 63.4% (many diffs)
15 human 81887 LAS1L LAS1 like ribosome biogenes... XM_005262305.4 64% 63.4% (many diffs)
16 human 81887 LAS1L LAS1 like ribosome biogenes... XM_017029880.2 57.8% 55.2% 0_1ins796;49_50ins110
17 human 81887 LAS1L LAS1 like ribosome biogenes... XM_017029879.2 56.5% 53.8% 0_1ins796;49_50ins110;137_187del
18 human 81887 LAS1L LAS1 like ribosome biogenes... XR_001755731.2 53.1% (many diffs)
19 human 81887 LAS1L LAS1 like ribosome biogenes... XR_001755733.2 53% 1_63del;1461_3107del;3862_4057del
20 human 81887 LAS1L LAS1 like ribosome biogenes... XR_001755732.2 52.3% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2217
ORF length:
2151
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaactttg tacaaaaaag
61ttggcatgtc gtgggaatcc ggggccgggc caggtctagg ttcccagggg atggatctcg
121tgtggagtgc gtggtacgga aagtgcgtta aagggaaagg gtcgttgcca ctctcggccc
181acggcatcgt ggtcgcctgg ctcagcaggg ccgagtggga ccaggtgacg gtttatctgt
241tctgtgacga ccataagttg cagcggtacg cgcttaaccg catcacggtg tggaggagca
301ggtcaggcaa cgaactccct ctggcagtgg cttctactgc tgacctgata cgctgtaagc
361tcttggatgt aactggtggc ttgggcactg atgaacttag actgctctat ggcatggcat
421tggtcaggtt tgtgaatctt atctcagaga ggaagacaaa gtttgccaag gtccccctca
481agtgtctggc tcaagaggta aatattccgg attggattgt tgaccttcgc catgagttga
541cccacaagaa aatgccccat ataaatgact gccgcagagg ctgctacttt gtcctggatt
601ggctccagaa gacctattgg tgccgccaac tggagaacag cctgagagag acctgggagt
661tggaggagtt cagggaaggg atagaggaag aggatcaaga ggaagataag aacattgttg
721ttgatgacat cacagaacag aaaccagagc ctcaggatga tgggaaaagt acggagtcag
781atgtaaaggc cgatggagac agcaaaggca gcgaagaggt ggattctcat tgcaaaaagg
841ccctgagtca taaagagcta tatgaaagag cccgagaact gctggtatca tacgaagagg
901agcagtttac ggtgctggag aaatttaggt atttacctaa ggccattaag gcgtggaata
961acccgtcccc acgtgtagaa tgtgtcctgg cagagctcaa gggcgttaca tgcgagaaca
1021gggaggctgt gctggatgct tttctggatg atggcttcct tgtccccaca tttgaacagt
1081tggcagcttt gcagatagaa tatgaagaaa acgtggactt gaatgacgtc ctggtgccaa
1141agccgttctc tcagttctgg cagcccctgc tcaggggcct gcactcccag aacttcacgc
1201aggccctatt ggagaggatg ctctctgaac tgccagcctt ggggatcagc gggatccggc
1261ctacctacat cctcagatgg accgttgaac tgatcgtggc caacaccaag actggacgga
1321atgctcgccg attttctgca ggccagtggg aagcaagaag gggctggagg ctgttcaact
1381gctccgcctc ccttgactgg ccccggatgg ttgagtcctg cttgggctca ccttgctggg
1441ccagccccca actccttcgg atcatcttca aagccatggg gcagggcctg ccagacgagg
1501agcaggagaa gctgctgcgc atctgttcca tttataccca gagtggagaa aacagcctgg
1561tgcaggaggg ctctgaggcc tcccccattg ggaagtctcc atatacacta gacagcctgt
1621attggagcgt caagccagcc agctccagct tcgggtctga agcaaaggcc cagcaacagg
1681aggagcaggg cagtgttaat gatgtcaagg aagaggagaa ggaggagaaa gaggtcttgc
1741cagaccaggt agaggaggag gaagaaaatg atgaccaaga ggaggaagag gaggatgaag
1801atgatgaaga tgatgaagag gaagacagaa tggaggtggg gcctttctct acagggcaag
1861agtcccccac tgccgagaat gctaggcttc tggcccagaa aagaggagct ttgcagggct
1921ctgcatggca ggttagctca gaagacgtgc gatgggacac atttccccta ggccgaatgc
1981caggtcagac cgaggaccca gcagagctca tgctggagaa ttatgacacc atgtatcttt
2041tggaccagcc tgtgctagag cagcggctgg aaccctcaac atgcaagact gacaccttgg
2101gcctgagctg tggtgtcggc agtggcaact gcagcaacag cagcagcagc aacttcgagg
2161gccttctctg gagccagggg cagctgcatg ggctcaaaac tggcctgcag ctcttctnnc
2221caactttctt gtacaaagtt ggcattataa gaaagcattg cttatcaatt tgttgcaacg
2281aac

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