Construct: ORF ccsbBroadEn_16051

Construct Description:

• Construct Type: ORF
• Other Identifiers: ORF016508.1_s300c1, BRDN0000397628
• DNA Barcode: None
• Epitope Tag: None
• Notes: No stop codon in insert

Originally Annotated References:

• Gene: RGS8 (85397)

Vector Information:

• Vector Backbone: pDONR223
• Pol II Cassette 1: n/a
• Pol II Cassette 2: n/a
• Selection Marker: n/a
• Visible Reporter: n/a
• Epitope Tag: n/a

Current transcripts matched by this ORF:

	Taxon	Gene	Symbol	Description	Transcript	Nuc. Match %	Prot. Match %	Match Diffs
1	human	85397	RGS8	regulator of G protein sign...	NM_001102450.2	100%	100%
2	human	85397	RGS8	regulator of G protein sign...	NM_001369564.2	100%	100%
3	human	85397	RGS8	regulator of G protein sign...	XM_005245555.4	100%	100%
4	human	85397	RGS8	regulator of G protein sign...	XM_011510090.2	100%	100%
5	human	85397	RGS8	regulator of G protein sign...	XM_017002635.1	100%	100%
6	human	85397	RGS8	regulator of G protein sign...	XM_017002637.2	100%	100%
7	human	85397	RGS8	regulator of G protein sign...	XM_011510091.3	97.2%	94.5%	(many diffs)
8	human	85397	RGS8	regulator of G protein sign...	NM_033345.3	89.1%	87.3%	(many diffs)
9	human	85397	RGS8	regulator of G protein sign...	XM_011510089.3	89.1%	87.3%	(many diffs)
10	human	85397	RGS8	regulator of G protein sign...	XM_017002631.2	89.1%	87.3%	(many diffs)
11	human	85397	RGS8	regulator of G protein sign...	XM_017002632.1	89.1%	87.3%	(many diffs)
12	human	85397	RGS8	regulator of G protein sign...	XM_017002633.1	89.1%	87.3%	(many diffs)
13	human	85397	RGS8	regulator of G protein sign...	XM_017002634.1	89.1%	87.3%	(many diffs)
14	mouse	67792	Rgs8	regulator of G-protein sign...	NM_026380.3	90.7%	98.3%	(many diffs)
15	mouse	67792	Rgs8	regulator of G-protein sign...	XM_006529818.3	90.7%	98.3%	(many diffs)
16	mouse	67792	Rgs8	regulator of G-protein sign...	XM_006529819.3	90.7%	98.3%	(many diffs)
17	mouse	67792	Rgs8	regulator of G-protein sign...	XM_006529820.3	90.7%	98.3%	(many diffs)
18	mouse	67792	Rgs8	regulator of G-protein sign...	XM_006529821.3	90.7%	98.3%	(many diffs)
19	mouse	67792	Rgs8	regulator of G-protein sign...	NM_001347115.1	88.3%	93.8%	(many diffs)
20	mouse	67792	Rgs8	regulator of G-protein sign...	XM_006529822.3	88.3%	93.8%	(many diffs)
21	mouse	67792	Rgs8	regulator of G-protein sign...	XM_006529824.3	88.3%	93.8%	(many diffs)
22	mouse	67792	Rgs8	regulator of G-protein sign...	XM_006529825.3	88.3%	93.8%	(many diffs)
23	mouse	67792	Rgs8	regulator of G-protein sign...	XM_006529826.3	88.3%	93.8%	(many diffs)
24	mouse	67792	Rgs8	regulator of G-protein sign...	XM_006529827.3	88.3%	93.8%	(many diffs)
25	mouse	67792	Rgs8	regulator of G-protein sign...	XM_006529828.3	88.3%	93.8%	(many diffs)
26	mouse	67792	Rgs8	regulator of G-protein sign...	XM_006529829.3	72.9%	74.4%	(many diffs)

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:

66

ORF end:

606

ORF length:

540

Sequence:

1	gttcgttgca acaaattgat gagcaatgct tttttataat gccaactttg tacaaaaaag
61	ttggcatggc ggccttactg atgccacgca ggaacaaagg gatgaggact cgactgggat
121	gcctgtctca caagtcagac tcgtgtagtg atttcacagc tattcttcca gacaaaccca
181	accgcgctct caagagatta tcgacagaag aagctacgag gtgggcagat tcctttgatg
241	tgcttctctc tcataagtat ggggtggctg cattccgtgc cttcttgaag acggagttca
301	gtgaggagaa cctggaattc tggttggcct gtgaggagtt caagaagacc aggtcaactg
361	caaaactggt ctctaaggcc cataggatct ttgaggagtt tgtggatgtg caggctccac
421	gggaggtaaa cattgacttc cagacccgag aagccacgag gaagaacctg caggagccat
481	ccctgacttg ctttgaccaa gcccaaggaa aagtacacag cctcatggag aaagactctt
541	accccaggtt cctgaggtcc aaaatgtact tagatctgct gtcccaaagc cagaggaggc
601	tcagttnncc aactttcttg tacaaagttg gcattataag aaagcattgc ttatcaattt
661	gttgcaacga ac

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