| Clone ID |
Target Seq |
Vector |
Matching Transcripts for Gene |
Match Regions[?]The region of the transcript where each match occurs (5UTR, CDS, 3UTR). NOTE: all matches to non-coding transcripts are labeled 3UTR, and the regions here are not necessarily listed in the same order as the matching transcripts. |
SDR Match %[?]Percent match of the "Specificity-Defining Region" of the hairpin target sequence to transcript RNA sequence. The SDR is defined as the initial 19 bases of the (21mer) target sequence.
| 84% | = 16/19 | | 89% | = 17/19 | | 95% | = 18/19 | | 100% | = 19/19 |
|
Intrinsic Score[?]Also called "original score", this assesses the target sequence for predicted cloneability and knockdown performance. |
Adjusted Score[?]Also called "specificity score", this is a function of the intrinsic score and the specificity factor. |
Matches other Human Gene? |
Orig. Target Gene[?]The gene that this construct was originally designed to target |
Addgene[?]Link to this entity's page in the Addgene catalog, if available |
| 1 |
TRCN0000371420 |
GTATGTACATTGACCATTAAA |
pLKO_005 |
NM_001143805.1, NM_001143806.1, NM_001143807.2, NM_001143808.1, NM_001143809.1, NM_001143810.1, NM_001143811.1, NM_001143812.1, NM_001143813.2, NM_001143814.2, NM_001143816.1, NM_001709.5, NM_170731.5, NM_170732.4, NM_170733.3, NM_170734.3, NM_170735.5, XM_011520280.2 |
CDS |
100% |
15.000 |
21.000 |
N |
BDNF |
n/a |
| 2 |
TRCN0000371421 |
ACAGTGGTTCTACAATCTATT |
pLKO_005 |
NM_001143805.1, NM_001143806.1, NM_001143807.2, NM_001143808.1, NM_001143809.1, NM_001143810.1, NM_001143811.1, NM_001143812.1, NM_001143813.2, NM_001143814.2, NM_001143816.1, NM_001709.5, NM_170731.5, NM_170732.4, NM_170733.3, NM_170734.3, NM_170735.5, XM_011520280.2 |
3UTR |
100% |
13.200 |
18.480 |
N |
BDNF |
n/a |
| 3 |
TRCN0000225731 |
GAATTGGCTGGCGATTCATAA |
pLKO_005 |
NM_001143805.1, NM_001143806.1, NM_001143807.2, NM_001143808.1, NM_001143809.1, NM_001143810.1, NM_001143811.1, NM_001143812.1, NM_001143813.2, NM_001143814.2, NM_001143816.1, NM_001709.5, NM_170731.5, NM_170732.4, NM_170733.3, NM_170734.3, NM_170735.5, XM_011520280.2 |
CDS |
100% |
13.200 |
9.240 |
N |
Bdnf |
n/a |
| 4 |
TRCN0000371395 |
GAATTGGCTGGCGATTCATAA |
pLKO_005 |
NM_001143805.1, NM_001143806.1, NM_001143807.2, NM_001143808.1, NM_001143809.1, NM_001143810.1, NM_001143811.1, NM_001143812.1, NM_001143813.2, NM_001143814.2, NM_001143816.1, NM_001709.5, NM_170731.5, NM_170732.4, NM_170733.3, NM_170734.3, NM_170735.5, XM_011520280.2 |
CDS |
100% |
13.200 |
9.240 |
N |
BDNF |
n/a |
| 5 |
TRCN0000225729 |
TTTCCTTACTATGGTTATTTC |
pLKO_005 |
NM_001143805.1, NM_001143806.1, NM_001143807.2, NM_001143808.1, NM_001143809.1, NM_001143810.1, NM_001143811.1, NM_001143812.1, NM_001143813.2, NM_001143814.2, NM_001143816.1, NM_001709.5, NM_170731.5, NM_170732.4, NM_170733.3, NM_170734.3, NM_170735.5, XM_011520280.2 |
CDS |
100% |
13.200 |
9.240 |
N |
Bdnf |
n/a |
| 6 |
TRCN0000225730 |
TGAGCGTGTGTGACAGTATTA |
pLKO_005 |
NM_001143805.1, NM_001143806.1, NM_001143807.2, NM_001143808.1, NM_001143809.1, NM_001143810.1, NM_001143811.1, NM_001143812.1, NM_001143813.2, NM_001143814.2, NM_001143816.1, NM_001709.5, NM_170731.5, NM_170732.4, NM_170733.3, NM_170734.3, NM_170735.5, XM_011520280.2 |
CDS |
100% |
13.200 |
7.920 |
N |
Bdnf |
n/a |
| 7 |
TRCN0000058210 |
GCCCTTACCATGGATAGCAAA |
pLKO.1 |
NM_001143805.1, NM_001143806.1, NM_001143807.2, NM_001143808.1, NM_001143809.1, NM_001143810.1, NM_001143811.1, NM_001143812.1, NM_001143813.2, NM_001143814.2, NM_001143816.1, NM_001709.5, NM_170731.5, NM_170732.4, NM_170733.3, NM_170734.3, NM_170735.5, XM_011520280.2 |
CDS |
100% |
4.950 |
3.960 |
N |
BDNF |
n/a |
| 8 |
TRCN0000058212 |
CTGTTGGATGAGGACCAGAAA |
pLKO.1 |
NM_001143805.1, NM_001143806.1, NM_001143807.2, NM_001143808.1, NM_001143809.1, NM_001143810.1, NM_001143811.1, NM_001143812.1, NM_001143813.2, NM_001143814.2, NM_001143816.1, NM_001709.5, NM_170731.5, NM_170732.4, NM_170733.3, NM_170734.3, NM_170735.5, XM_011520280.2 |
CDS |
100% |
4.950 |
3.465 |
N |
BDNF |
n/a |
| 9 |
TRCN0000058211 |
GAAGCAAACATCCGAGGACAA |
pLKO.1 |
NM_001143805.1, NM_001143806.1, NM_001143807.2, NM_001143808.1, NM_001143809.1, NM_001143810.1, NM_001143811.1, NM_001143812.1, NM_001143813.2, NM_001143814.2, NM_001143816.1, NM_001709.5, NM_170731.5, NM_170732.4, NM_170733.3, NM_170734.3, NM_170735.5, XM_011520280.2 |
CDS |
100% |
4.050 |
2.835 |
N |
BDNF |
n/a |
| 10 |
TRCN0000058208 |
GCTCAGTAGTCAAGTGCCTTT |
pLKO.1 |
NM_001143805.1, NM_001143806.1, NM_001143807.2, NM_001143808.1, NM_001143809.1, NM_001143810.1, NM_001143811.1, NM_001143812.1, NM_001143813.2, NM_001143814.2, NM_001143816.1, NM_001709.5, NM_170731.5, NM_170732.4, NM_170733.3, NM_170734.3, NM_170735.5, XM_011520280.2 |
CDS |
100% |
4.050 |
2.835 |
N |
BDNF |
n/a |
| 11 |
TRCN0000058209 |
GCAATACTTCTACGAGACCAA |
pLKO.1 |
NM_001143805.1, NM_001143806.1, NM_001143807.2, NM_001143808.1, NM_001143809.1, NM_001143810.1, NM_001143811.1, NM_001143812.1, NM_001143813.2, NM_001143814.2, NM_001143816.1, NM_001709.5, NM_170731.5, NM_170732.4, NM_170733.3, NM_170734.3, NM_170735.5, XM_011520280.2 |
CDS |
100% |
2.640 |
1.848 |
N |
BDNF |
n/a |
| 12 |
TRCN0000166364 |
CACACACACACACACACACAA |
pLKO.1 |
NM_001143816.1, NM_170735.5 |
5UTR |
100% |
4.950 |
2.475 |
Y |
KAAG1 |
n/a |
| 13 |
TRCN0000143693 |
GAGCCAAATGTGGCATTTGAA |
pLKO.1 |
NM_001143805.1, NM_001143806.1, NM_001143807.2, NM_001143808.1, NM_001143809.1, NM_001143810.1, NM_001143811.1, NM_001143812.1, NM_001143813.2, NM_001143814.2, NM_001143816.1, NM_001709.5, NM_170731.5, NM_170732.4, NM_170733.3, NM_170734.3, NM_170735.5, XM_011520280.2 |
3UTR |
100% |
0.563 |
0.281 |
Y |
PIGT |
n/a |
| 14 |
TRCN0000280842 |
GAGCCAAATGTGGCATTTGAA |
pLKO_005 |
NM_001143805.1, NM_001143806.1, NM_001143807.2, NM_001143808.1, NM_001143809.1, NM_001143810.1, NM_001143811.1, NM_001143812.1, NM_001143813.2, NM_001143814.2, NM_001143816.1, NM_001709.5, NM_170731.5, NM_170732.4, NM_170733.3, NM_170734.3, NM_170735.5, XM_011520280.2 |
3UTR |
100% |
0.563 |
0.281 |
Y |
PIGT |
n/a |
Download CSV