Transcript: Human NM_000620.5

Homo sapiens nitric oxide synthase 1 (NOS1), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-09-22
Taxon:
Homo sapiens (human)
Gene:
NOS1 (4842)
Length:
12007
CDS:
536..4840

Additional Resources:

NCBI RefSeq record:
NM_000620.5
NBCI Gene record:
NOS1 (4842)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_000620.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000045424 CGTCTCTTCAAGCGCAAAGTT pLKO.1 590 CDS 100% 5.625 7.875 N NOS1 n/a
2 TRCN0000416166 TCGTCCTCAACAACCCATATT pLKO_005 1353 CDS 100% 13.200 10.560 N NOS1 n/a
3 TRCN0000045426 CCACGGGATGTTCAACTACAT pLKO.1 1855 CDS 100% 4.950 3.960 N NOS1 n/a
4 TRCN0000431409 GCAATCCAAGATAGATCATAT pLKO_005 4402 CDS 100% 13.200 9.240 N NOS1 n/a
5 TRCN0000045427 CGGCTGTGCTTTGATGGAAAT pLKO.1 3013 CDS 100% 10.800 7.560 N NOS1 n/a
6 TRCN0000045425 CGGCTTTAGGTGTCATCAGTA pLKO.1 3762 CDS 100% 4.950 3.465 N NOS1 n/a
7 TRCN0000045423 GCCTTCATTGAAGAGAGCAAA pLKO.1 4790 CDS 100% 4.950 3.465 N NOS1 n/a
8 TRCN0000166364 CACACACACACACACACACAA pLKO.1 11727 3UTR 100% 4.950 2.475 Y KAAG1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_000620.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

No results found.