Transcript: Human NM_001009944.3

Homo sapiens polycystin 1, transient receptor potential channel interacting (PKD1), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-09-03
Taxon:
Homo sapiens (human)
Gene:
PKD1 (5310)
Length:
14140
CDS:
210..13121

Additional Resources:

NCBI RefSeq record:
NM_001009944.3
NBCI Gene record:
PKD1 (5310)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001149544 GCGGCCCTCCAAGTACACGT pXPR_003 AGG 7159 55% 17 1.2358 PKD1 PKD1 76841
2 BRDN0001148399 GTTGGTGCCATCCCTAACCA pXPR_003 CGG 4966 38% 15 0.986 PKD1 PKD1 76842
3 BRDN0001149263 CGTGAACTACAACGTAACCG pXPR_003 TGG 3034 23% 13 0.2583 PKD1 PKD1 76843
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001009944.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000062322 GTCCGTCTTTGGCAAGACATT pLKO.1 12245 CDS 100% 0.495 0.347 N PKD1 n/a
2 TRCN0000062319 GCCATTTACCACCCATAGCTT pLKO.1 5501 CDS 100% 3.000 1.800 N PKD1 n/a
3 TRCN0000417410 TGACCGTGCTGGCATCTAATG pLKO_005 3517 CDS 100% 10.800 5.400 Y PKD1 n/a
4 TRCN0000062320 CGAGGAGTTCTGTGTCTACAA pLKO.1 7775 CDS 100% 4.950 2.475 Y PKD1 n/a
5 TRCN0000062318 GCTTTGTGTTTGTCGTGTCAT pLKO.1 6895 CDS 100% 4.950 2.475 Y PKD1 n/a
6 TRCN0000062321 GCTATGGAGAGAGTTCCTCTT pLKO.1 2252 CDS 100% 4.050 2.025 Y PKD1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001009944.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

No results found.