Transcript: Human NM_004730.4

Homo sapiens eukaryotic translation termination factor 1 (ETF1), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-09-27
Taxon:
Homo sapiens (human)
Gene:
ETF1 (2107)
Length:
3682
CDS:
159..1472

Additional Resources:

NCBI RefSeq record:
NM_004730.4
NBCI Gene record:
ETF1 (2107)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_004730.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000145565 GCCTACAACTAAGTTCCTAAA pLKO.1 1678 3UTR 100% 10.800 15.120 N ETF1 n/a
2 TRCN0000350927 GCCTACAACTAAGTTCCTAAA pLKO_005 1678 3UTR 100% 10.800 15.120 N ETF1 n/a
3 TRCN0000191439 CATAACTATGTTCGGAAAGTA pLKO.1 753 CDS 100% 5.625 7.875 N Etf1 n/a
4 TRCN0000279011 CATAACTATGTTCGGAAAGTA pLKO_005 753 CDS 100% 5.625 7.875 N Etf1 n/a
5 TRCN0000142557 GCCATTGTAAATGGGTCTGAT pLKO.1 3286 3UTR 100% 4.950 6.930 N ETF1 n/a
6 TRCN0000144191 CGACATAACTATGTTCGGAAA pLKO.1 750 CDS 100% 4.050 5.670 N ETF1 n/a
7 TRCN0000338685 CGACATAACTATGTTCGGAAA pLKO_005 750 CDS 100% 4.050 5.670 N ETF1 n/a
8 TRCN0000145600 CAGCACTACTTTCAGATGATA pLKO.1 568 CDS 100% 5.625 3.938 N ETF1 n/a
9 TRCN0000141242 CCTCTCCAACGTGAAATTCAT pLKO.1 980 CDS 100% 5.625 3.938 N ETF1 n/a
10 TRCN0000142237 GAAGGGTCTCAGTTTGTGAAA pLKO.1 1350 CDS 100% 4.950 3.465 N ETF1 n/a
11 TRCN0000145345 GCCATTACATCTGTACAACAA pLKO.1 378 CDS 100% 4.950 3.465 N ETF1 n/a
12 TRCN0000145405 GCTGTTCTTTACTGACTTGAT pLKO.1 2931 3UTR 100% 4.950 3.465 N ETF1 n/a
13 TRCN0000139839 CCAGATTTCACGAGTGGCAAA pLKO.1 287 CDS 100% 4.050 2.835 N ETF1 n/a
14 TRCN0000142136 GCACAAATTCACTGTGGATCT pLKO.1 665 CDS 100% 4.050 2.835 N ETF1 n/a
15 TRCN0000338749 GCACAAATTCACTGTGGATCT pLKO_005 665 CDS 100% 4.050 2.835 N ETF1 n/a
16 TRCN0000139490 CGGATGAGTTTGGAACTGCAT pLKO.1 316 CDS 100% 2.640 1.848 N ETF1 n/a
17 TRCN0000338748 CGGATGAGTTTGGAACTGCAT pLKO_005 316 CDS 100% 2.640 1.848 N ETF1 n/a
18 TRCN0000141821 GCTACGTTGGAAATTGTCACA pLKO.1 1317 CDS 100% 2.640 1.848 N ETF1 n/a
19 TRCN0000140357 GCTTTGGAAATGGGAGCTGTA pLKO.1 1089 CDS 100% 4.050 2.430 N ETF1 n/a
20 TRCN0000189586 CCTCCAAATGGTCTGGTTGTT pLKO.1 423 CDS 100% 4.950 3.465 N Etf1 n/a
21 TRCN0000279074 CCTCCAAATGGTCTGGTTGTT pLKO_005 423 CDS 100% 4.950 3.465 N Etf1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_004730.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

No results found.