Transcript: Human NM_007118.4

Homo sapiens trio Rho guanine nucleotide exchange factor (TRIO), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-08-22
Taxon:
Homo sapiens (human)
Gene:
TRIO (7204)
Length:
11460
CDS:
385..9678

Additional Resources:

NCBI RefSeq record:
NM_007118.4
NBCI Gene record:
TRIO (7204)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001148044 ATGGTGTAAAGCTTGCGGTG pXPR_003 AGG 1405 15% 8 0.3872 TRIO TRIO 78032
2 BRDN0001147635 AACGAGCTGACCATCCGACG pXPR_003 GGG 5033 54% 34 0.3491 TRIO TRIO 78033
3 BRDN0001487141 GGGGGCTCGGAATATGATCG pXPR_003 AGG 769 8% 5 -0.4954 TRIO TRIO 78034
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_007118.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000195106 CCTTCAACCCTTCGGATAATT pLKO.1 6206 CDS 100% 15.000 21.000 N TRIO n/a
2 TRCN0000196770 GATTCCAACAAATCGAGTAAA pLKO.1 4135 CDS 100% 13.200 18.480 N TRIO n/a
3 TRCN0000010561 GCTTCCCAGATGACTACTTTA pLKO.1 9413 CDS 100% 13.200 18.480 N TRIO n/a
4 TRCN0000277906 GCTTCCCAGATGACTACTTTA pLKO_005 9413 CDS 100% 13.200 18.480 N TRIO n/a
5 TRCN0000254107 TCGACCTATCCGTAGCATTAA pLKO_005 9621 CDS 100% 13.200 18.480 N Trio n/a
6 TRCN0000000873 GCAACGTGGATTCATGGTGTA pLKO.1 1760 CDS 100% 4.050 5.670 N TRIO n/a
7 TRCN0000195292 CCACGAAGAATGGATTGAAAT pLKO.1 1011 CDS 100% 13.200 9.240 N TRIO n/a
8 TRCN0000277765 CCACGAAGAATGGATTGAAAT pLKO_005 1011 CDS 100% 13.200 9.240 N TRIO n/a
9 TRCN0000196250 GCATTGCTGTATCACAGTATT pLKO.1 9897 3UTR 100% 13.200 9.240 N TRIO n/a
10 TRCN0000277841 GCATTGCTGTATCACAGTATT pLKO_005 9897 3UTR 100% 13.200 9.240 N TRIO n/a
11 TRCN0000196751 GATGAAATGTAGGCCTTACTT pLKO.1 10245 3UTR 100% 5.625 3.938 N TRIO n/a
12 TRCN0000297115 GATGAAATGTAGGCCTTACTT pLKO_005 10245 3UTR 100% 5.625 3.938 N TRIO n/a
13 TRCN0000000872 GCACAGAACACATACACCAAT pLKO.1 2233 CDS 100% 4.950 3.465 N TRIO n/a
14 TRCN0000000874 GCTTCTCAATCCCAACTACAT pLKO.1 8403 CDS 100% 4.950 3.465 N TRIO n/a
15 TRCN0000000871 CAAGCAAACATAACTGATCAG pLKO.1 9750 3UTR 100% 4.050 2.835 N TRIO n/a
16 TRCN0000277766 CAAGCAAACATAACTGATCAG pLKO_005 9750 3UTR 100% 4.050 2.835 N TRIO n/a
17 TRCN0000195713 CCAGACTGACTTCCTTCATTG pLKO.1 9575 CDS 100% 10.800 6.480 N TRIO n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_007118.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

No results found.