Transcript: Mouse NM_009408.2

Mus musculus topoisomerase (DNA) I (Top1), mRNA.

Source:
NCBI, updated 2017-05-14
Taxon:
Mus musculus (mouse)
Gene:
Top1 (21969)
Length:
3859
CDS:
334..2637

Additional Resources:

NCBI RefSeq record:
NM_009408.2
NBCI Gene record:
Top1 (21969)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_009408.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000415074 ACCTAAGCAAATGCGACTTTA pLKO_005 1226 CDS 100% 13.200 18.480 N Top1 n/a
2 TRCN0000011886 CCGCCACGAATTAAAGATGAA pLKO.1 676 CDS 100% 4.950 6.930 N Top1 n/a
3 TRCN0000011887 GCAGTCTAAGATTGATGCCAA pLKO.1 2280 CDS 100% 2.640 3.696 N Top1 n/a
4 TRCN0000421095 GAAGATGATGCTGATTATAAA pLKO_005 763 CDS 100% 15.000 10.500 N Top1 n/a
5 TRCN0000003988 CATAGCAACAGTGAACATAAA pLKO.1 484 CDS 100% 13.200 9.240 N TOP1 n/a
6 TRCN0000318511 CATAGCAACAGTGAACATAAA pLKO_005 484 CDS 100% 13.200 9.240 N TOP1 n/a
7 TRCN0000011884 CCAGCGAAGATTCTATCTTAT pLKO.1 2176 CDS 100% 13.200 9.240 N Top1 n/a
8 TRCN0000413918 GACTCAATCAGATACTATAAC pLKO_005 1936 CDS 100% 13.200 9.240 N Top1 n/a
9 TRCN0000434033 AGATCGAGAACACCGGCATAA pLKO_005 420 CDS 100% 10.800 7.560 N Top1 n/a
10 TRCN0000011885 CGATTGAATGATTCTCACAAA pLKO.1 382 CDS 100% 4.950 3.465 N Top1 n/a
11 TRCN0000011883 CCACAAGTCTTAACAAACCAA pLKO.1 2731 3UTR 100% 3.000 2.100 N Top1 n/a
12 TRCN0000435820 AGGAGTATGTGGTGGAATTTG pLKO_005 1901 CDS 100% 13.200 7.920 N Top1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_009408.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

No results found.