Transcript: Human NM_032888.4

Homo sapiens collagen type XXVII alpha 1 chain (COL27A1), mRNA.

Source:
NCBI, updated 2019-08-22
Taxon:
Homo sapiens (human)
Gene:
COL27A1 (85301)
Length:
7813
CDS:
415..5997

Additional Resources:

NCBI RefSeq record:
NM_032888.4
NBCI Gene record:
COL27A1 (85301)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_032888.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000430252 ATAACCAGCCATGCCAGTAAG pLKO_005 1804 CDS 100% 10.800 15.120 N COL27A1 n/a
2 TRCN0000443451 TAGGAGTGTTGGGTCCGATTG pLKO_005 2888 CDS 100% 6.000 8.400 N COL27A1 n/a
3 TRCN0000437530 CCGCATGCAGTCCAGTTTGAA pLKO_005 1024 CDS 100% 5.625 7.875 N COL27A1 n/a
4 TRCN0000116604 CCTATCCAATTGCAACAAGAT pLKO.1 5275 CDS 100% 4.950 6.930 N COL27A1 n/a
5 TRCN0000438144 ATCAGCCGGGTCCAGATGAAT pLKO_005 5644 CDS 100% 5.625 3.938 N COL27A1 n/a
6 TRCN0000116603 CAGGGATTTATGGGATTCATT pLKO.1 3391 CDS 100% 5.625 3.938 N COL27A1 n/a
7 TRCN0000116605 CGCTCAACCATCACAGAAGAT pLKO.1 1470 CDS 100% 4.950 3.465 N COL27A1 n/a
8 TRCN0000116606 CCAGTTCAGTATCTACCCTGT pLKO.1 1056 CDS 100% 2.160 1.080 Y COL27A1 n/a
9 TRCN0000425740 CTTGACCACTGCCACACCAAT pLKO_005 1227 CDS 100% 4.950 3.465 N Defb2 n/a
Download CSV

shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_032888.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

Download CSV

All ORF constructs matching this transcript

No results found.