Transcript: Human NM_052859.4

Homo sapiens RFT1 homolog (RFT1), mRNA.

Source:
NCBI, updated 2019-08-22
Taxon:
Homo sapiens (human)
Gene:
RFT1 (91869)
Length:
5081
CDS:
36..1661

Additional Resources:

NCBI RefSeq record:
NM_052859.4
NBCI Gene record:
RFT1 (91869)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_052859.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000243304 ACTCTATATGGCTAGTATTAC pLKO_005 2571 3UTR 100% 13.200 18.480 N RFT1 n/a
2 TRCN0000243305 GAAATCGTTGGCGTAGTAAAT pLKO_005 159 CDS 100% 13.200 18.480 N RFT1 n/a
3 TRCN0000166936 GCGTAGTAAATGTAAGACTAA pLKO.1 169 CDS 100% 4.950 6.930 N RFT1 n/a
4 TRCN0000243303 ACAGATCTGTTACCCAATATT pLKO_005 699 CDS 100% 15.000 10.500 N RFT1 n/a
5 TRCN0000243301 CAAGAAATGGAGCGTTTATAA pLKO_005 721 CDS 100% 15.000 10.500 N RFT1 n/a
6 TRCN0000243302 GGAGGTCGACAGGTACAATTT pLKO_005 1232 CDS 100% 13.200 9.240 N RFT1 n/a
7 TRCN0000167610 GCTCCAACAAACTATATCTTA pLKO.1 1982 3UTR 100% 5.625 3.938 N RFT1 n/a
8 TRCN0000155836 CCCAAAGTGCTGGGATTACAA pLKO.1 4667 3UTR 100% 5.625 2.813 Y KLHL30 n/a
9 TRCN0000141025 CCCAAAGTGCTGGGATTACTT pLKO.1 4667 3UTR 100% 5.625 2.813 Y EID2B n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_052859.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

No results found.