Transcript: Mouse NM_146086.2

Mus musculus phosphodiesterase 6A, cGMP-specific, rod, alpha (Pde6a), mRNA.

Source:
NCBI, updated 2017-05-20
Taxon:
Mus musculus (mouse)
Gene:
Pde6a (225600)
Length:
4230
CDS:
107..2689

Additional Resources:

NCBI RefSeq record:
NM_146086.2
NBCI Gene record:
Pde6a (225600)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_146086.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000114945 CATCATTTGGAGTTTGGCAAA pLKO.1 1994 CDS 100% 4.050 5.670 N Pde6a n/a
2 TRCN0000114944 CCCGATTTCATGAGGAGATTA pLKO.1 2472 CDS 100% 13.200 9.240 N Pde6a n/a
3 TRCN0000114941 GCAGGCATCTATATCATCAAA pLKO.1 2792 3UTR 100% 5.625 3.938 N Pde6a n/a
4 TRCN0000114943 GCTGAGAAATGCACATTCAAT pLKO.1 326 CDS 100% 5.625 3.938 N Pde6a n/a
5 TRCN0000008603 GCCATAATCATGGCTGTGAAT pLKO.1 674 CDS 100% 0.495 0.347 N PDE6A n/a
6 TRCN0000114942 CCGGAGAATCACTTACCACAA pLKO.1 1765 CDS 100% 4.050 2.430 N Pde6a n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_146086.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

No results found.