Transcript: Mouse NM_175517.3

Mus musculus family with sequence similarity 221, member B (Fam221b), mRNA.

Source:
NCBI, updated 2017-05-13
Taxon:
Mus musculus (mouse)
Gene:
Fam221b (242408)
Length:
1763
CDS:
267..1730

Additional Resources:

NCBI RefSeq record:
NM_175517.3
NBCI Gene record:
Fam221b (242408)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_175517.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000281612 GTCTCGTCAACAGCCAATATC pLKO_005 801 CDS 100% 13.200 18.480 N Fam221b n/a
2 TRCN0000264332 TGGCGCTGCCCGCATTATTTA pLKO_005 1278 CDS 100% 15.000 10.500 N Fam221b n/a
3 TRCN0000264333 AGGGCCCAATGTCGCTGTAAA pLKO_005 1500 CDS 100% 13.200 9.240 N Fam221b n/a
4 TRCN0000201206 GCAAAGGAACAGCCTTCTTTA pLKO.1 312 CDS 100% 13.200 9.240 N Fam221b n/a
5 TRCN0000264334 GCTCTCACCAACAGCCAATAT pLKO_005 662 CDS 100% 13.200 9.240 N Fam221b n/a
6 TRCN0000264335 TATGACAGTCCTATCTCTAAA pLKO_005 621 CDS 100% 13.200 9.240 N Fam221b n/a
7 TRCN0000217029 CAAGGAGGACGAATCTACAAA pLKO.1 995 CDS 100% 5.625 3.938 N Fam221b n/a
8 TRCN0000192863 GCTATGACAGTCCTATCTCTA pLKO.1 619 CDS 100% 4.950 2.970 N Fam221b n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_175517.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

No results found.