Transcript: Human NM_181709.4

Homo sapiens refilin A (RFLNA), transcript variant 2, mRNA.

Source:
NCBI, updated 2019-04-23
Taxon:
Homo sapiens (human)
Gene:
RFLNA (144347)
Length:
2160
CDS:
244..651

Additional Resources:

NCBI RefSeq record:
NM_181709.4
NBCI Gene record:
RFLNA (144347)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_181709.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000263831 GAGCTCAGCTGTCGGTTTAAA pLKO_005 1563 3UTR 100% 15.000 7.500 Y RFLNA n/a
2 TRCN0000263833 GTACGCCTCGGAGAAGCATTT pLKO_005 336 CDS 100% 10.800 5.400 Y RFLNA n/a
3 TRCN0000282826 CAAGCATGCCAGGAGCACTTT pLKO_005 516 CDS 100% 4.950 2.475 Y RFLNA n/a
4 TRCN0000172961 GAGAAGCATTTCCAGGACAAG pLKO.1 346 CDS 100% 4.050 2.025 Y RFLNA n/a
5 TRCN0000263832 CAGGACAAGGTCTTCTATGCG pLKO_005 358 CDS 100% 2.640 1.320 Y RFLNA n/a
6 TRCN0000172744 GAAGCATTTCCAGGACAAGGT pLKO.1 348 CDS 100% 2.640 1.320 Y RFLNA n/a
7 TRCN0000282823 ACGCATGAGATCCGCTGCAAC pLKO_005 304 CDS 100% 1.350 0.675 Y RFLNA n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_181709.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

No results found.