Transcript: Human XM_005263920.3

PREDICTED: Homo sapiens ring finger protein 149 (RNF149), transcript variant X1, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
RNF149 (284996)
Length:
4971
CDS:
112..1398

Additional Resources:

NCBI RefSeq record:
XM_005263920.3
NBCI Gene record:
RNF149 (284996)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_005263920.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000221006 ACCATGATGATTATCTCGTTA pLKO.1 739 CDS 100% 4.950 6.930 N RNF149 n/a
2 TRCN0000221007 CGTGGTAAACATCGAGTACGT pLKO.1 231 CDS 100% 2.640 3.696 N RNF149 n/a
3 TRCN0000221008 CAGGAAATATAGTGGTCATTA pLKO.1 575 CDS 100% 13.200 9.240 N RNF149 n/a
4 TRCN0000414888 CATCAAAGCCCTAGGATATTG pLKO_005 1050 CDS 100% 13.200 9.240 N RNF149 n/a
5 TRCN0000221004 CCAGATGATGACGGAAGTGAT pLKO.1 1156 CDS 100% 4.950 3.465 N RNF149 n/a
6 TRCN0000221005 GAATTGATGTTGATGCTGAAA pLKO.1 893 CDS 100% 4.950 3.465 N RNF149 n/a
7 TRCN0000155836 CCCAAAGTGCTGGGATTACAA pLKO.1 3076 3UTR 100% 5.625 2.813 Y KLHL30 n/a
8 TRCN0000141025 CCCAAAGTGCTGGGATTACTT pLKO.1 3076 3UTR 100% 5.625 2.813 Y EID2B n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_005263920.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

No results found.