Transcript: Mouse XM_006517056.2

PREDICTED: Mus musculus calpastatin (Cast), transcript variant X1, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Cast (12380)
Length:
5204
CDS:
365..2728

Additional Resources:

NCBI RefSeq record:
XM_006517056.2
NBCI Gene record:
Cast (12380)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006517056.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000080114 CCTCCTGATTATCGACTAGAA pLKO.1 2030 CDS 100% 4.950 6.930 N Cast n/a
2 TRCN0000316248 CCTCCTGATTATCGACTAGAA pLKO_005 2030 CDS 100% 4.950 6.930 N Cast n/a
3 TRCN0000080117 CCTCCAGAGTATAGGAAACTT pLKO.1 1211 CDS 100% 5.625 4.500 N Cast n/a
4 TRCN0000316316 CCTCCAGAGTATAGGAAACTT pLKO_005 1211 CDS 100% 5.625 4.500 N Cast n/a
5 TRCN0000080113 GCCTGTAAATCTCATTAGTAT pLKO.1 3069 3UTR 100% 5.625 3.938 N Cast n/a
6 TRCN0000316317 GCCTGTAAATCTCATTAGTAT pLKO_005 3069 3UTR 100% 5.625 3.938 N Cast n/a
7 TRCN0000080115 CCACCATATACTGGACCAGTA pLKO.1 1130 CDS 100% 4.050 2.835 N Cast n/a
8 TRCN0000080116 GCCCTCTCAGAAGATTTGGAT pLKO.1 2543 CDS 100% 3.000 2.100 N Cast n/a
9 TRCN0000316247 GCCCTCTCAGAAGATTTGGAT pLKO_005 2543 CDS 100% 3.000 2.100 N Cast n/a
10 TRCN0000106535 GCCTGGTCTACAAAGTGAGTT pLKO.1 4212 3UTR 100% 4.950 2.475 Y Gad2 n/a
11 TRCN0000054408 ACGCCTTTAATCCCAGCACTT pLKO.1 4153 3UTR 100% 4.050 2.025 Y Mtif2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006517056.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

No results found.