Transcript: Mouse XM_006519127.3

PREDICTED: Mus musculus coiled-coil domain containing 66 (Ccdc66), transcript variant X1, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Ccdc66 (320234)
Length:
4461
CDS:
148..2856

Additional Resources:

NCBI RefSeq record:
XM_006519127.3
NBCI Gene record:
Ccdc66 (320234)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006519127.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000215849 CGAACAAATGAGATCTATTAT pLKO.1 2560 CDS 100% 15.000 21.000 N Ccdc66 n/a
2 TRCN0000216852 GGTGCACAAGTGGATTATAAG pLKO.1 1747 CDS 100% 13.200 18.480 N Ccdc66 n/a
3 TRCN0000192743 GCAGACAGATGACGTAAACTT pLKO.1 1854 CDS 100% 5.625 7.875 N Ccdc66 n/a
4 TRCN0000191949 GAAACGAATCTCATACCCTTA pLKO.1 2797 CDS 100% 4.050 5.670 N Ccdc66 n/a
5 TRCN0000217446 GACTTTAGGAGATGGTCATAC pLKO.1 2490 CDS 100% 10.800 8.640 N Ccdc66 n/a
6 TRCN0000192494 CGGTAACAGAGAAGGGTATTA pLKO.1 1910 CDS 100% 13.200 9.240 N Ccdc66 n/a
7 TRCN0000215671 GATCCACTTCTTAATCCTAAA pLKO.1 2689 CDS 100% 10.800 7.560 N Ccdc66 n/a
8 TRCN0000191598 GCTATTTGACTCTGACCATAT pLKO.1 2664 CDS 100% 10.800 7.560 N Ccdc66 n/a
9 TRCN0000200694 GAACTCAACAAACTCAAAGTA pLKO.1 2420 CDS 100% 5.625 3.938 N Ccdc66 n/a
10 TRCN0000192663 GCTTCACTTAGTGGAGAGAAA pLKO.1 2232 CDS 100% 4.950 3.465 N Ccdc66 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006519127.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

No results found.