Transcript: Human XM_011533977.2

PREDICTED: Homo sapiens roundabout guidance receptor 1 (ROBO1), transcript variant X2, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
ROBO1 (6091)
Length:
6789
CDS:
151..5115

Additional Resources:

NCBI RefSeq record:
XM_011533977.2
NBCI Gene record:
ROBO1 (6091)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_011533977.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000330248 TGACACATGACGCCAGATAAA pLKO_005 5179 3UTR 100% 13.200 10.560 N ROBO1 n/a
2 TRCN0000330183 ATAGCGAAGAGTACAACATTT pLKO_005 3752 CDS 100% 13.200 9.240 N ROBO1 n/a
3 TRCN0000330182 ATCATCACAAAGGCATATTTG pLKO_005 1468 CDS 100% 13.200 9.240 N ROBO1 n/a
4 TRCN0000330247 ATCTCATCCAAGAGGATATTC pLKO_005 4892 CDS 100% 13.200 9.240 N ROBO1 n/a
5 TRCN0000060413 GCAGAAATACAGTCACATTAT pLKO.1 1877 CDS 100% 13.200 9.240 N ROBO1 n/a
6 TRCN0000060417 GCCACCAGCAAGGATGTATTT pLKO.1 3813 CDS 100% 13.200 9.240 N ROBO1 n/a
7 TRCN0000330180 TTGCTGCCGAGTGGATCTTTA pLKO_005 511 CDS 100% 13.200 9.240 N ROBO1 n/a
8 TRCN0000060416 GCCCACCATTTCATGGAAGAA pLKO.1 747 CDS 100% 4.950 3.465 N ROBO1 n/a
9 TRCN0000060414 GCTGGCAAATATGTTTGTGTT pLKO.1 853 CDS 100% 4.950 3.465 N ROBO1 n/a
10 TRCN0000060415 GCAGACAAAGAGAACAAGCAA pLKO.1 5000 CDS 100% 3.000 2.100 N ROBO1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011533977.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

No results found.