Transcript: Human XM_011545712.2

PREDICTED: Homo sapiens endoplasmic reticulum to nucleus signaling 2 (ERN2), transcript variant X5, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
ERN2 (10595)
Length:
1966
CDS:
62..1735

Additional Resources:

NCBI RefSeq record:
XM_011545712.2
NBCI Gene record:
ERN2 (10595)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001162277 GAGACCACTTCTTACCACCA pXPR_003 GGG 931 56% 9 1.0612 ERN2 ERN2 75848
2 BRDN0001145316 GTGGAGACTTCCATCCAAGG pXPR_003 TGG 136 8% 2 0.8463 ERN2 ERN2 75845
3 BRDN0001147211 TTGGGGACCCAAAAACAACA pXPR_003 GGG 293 18% 4 0.2928 ERN2 ERN2 75846
4 BRDN0001162553 CTGAGCAACTCACCGTAGTG pXPR_003 GGG 1673 100% 14 -0.8262 ERN2 ERN2 75847
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_011545712.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000195361 CGTCATCGAAGGACCAATGTA pLKO.1 265 CDS 100% 5.625 7.875 N ERN2 n/a
2 TRCN0000021432 ACTGGCTTCTATGTCTCTAAA pLKO.1 944 CDS 100% 13.200 9.240 N ERN2 n/a
3 TRCN0000195136 CTGGCTTCTATGTCTCTAAAG pLKO.1 945 CDS 100% 10.800 7.560 N ERN2 n/a
4 TRCN0000195546 CTGCAGACTTTGCTCACATCT pLKO.1 1598 CDS 100% 4.950 3.465 N ERN2 n/a
5 TRCN0000179143 GAAGGATGAAACTGGCTTCTA pLKO.1 934 CDS 100% 4.950 3.465 N ERN2 n/a
6 TRCN0000147669 GAAGATTTCCTTCAATCCCAA pLKO.1 1740 3UTR 100% 2.640 1.848 N ERN2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011545712.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

No results found.