Transcript: Human XM_017024753.2

PREDICTED: Homo sapiens cyclin dependent kinase 12 (CDK12), transcript variant X24, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
CDK12 (51755)
Length:
5718
CDS:
562..4347

Additional Resources:

NCBI RefSeq record:
XM_017024753.2
NBCI Gene record:
CDK12 (51755)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001145847 CTAGCAGTCCCATTAAGTCA pXPR_003 TGG 2453 65% 6 0.3501 CDK12 CDK12 75917
2 BRDN0001147294 ACTGACCGACTGCCTTCTCG pXPR_003 AGG 811 21% 1 0.207 CDK12 CDK12 75916
3 BRDN0001147156 TGGCCTTCAAACTAGACCGA pXPR_003 AGG 276 7% 1 0.1532 CDK12 CDK12 75915
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017024753.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000237811 GCGTTCCCGGGACTTACTAAA pLKO_005 912 CDS 100% 13.200 18.480 N CDK12 n/a
2 TRCN0000364126 TTGGACTTGCTCGGCTCTATA pLKO_005 3191 CDS 100% 13.200 18.480 N CDK12 n/a
3 TRCN0000237812 ATCGAAGCACAAGCGGCATAA pLKO_005 678 CDS 100% 10.800 15.120 N CDK12 n/a
4 TRCN0000364125 ATCGATGAAGGACCGGATATC pLKO_005 984 CDS 100% 10.800 15.120 N CDK12 n/a
5 TRCN0000368334 CAGTTCACGCAGTCGTCATTC pLKO_005 1683 CDS 100% 10.800 15.120 N CDK12 n/a
6 TRCN0000197022 GATCGATGAAGGACCGGATAT pLKO.1 983 CDS 100% 10.800 15.120 N CDK12 n/a
7 TRCN0000368335 ATTTCCTGCATCGGGATATTA pLKO_005 3119 CDS 100% 15.000 12.000 N CDK12 n/a
8 TRCN0000364122 GGGCACAAAGACCGGAGTAAA pLKO_005 1138 CDS 100% 13.200 10.560 N CDK12 n/a
9 TRCN0000196423 GAAGTAGAAGTCCTGCATATT pLKO.1 1625 CDS 100% 13.200 9.240 N CDK12 n/a
10 TRCN0000364124 GGAACCTATGGCCAAGTATAT pLKO_005 2764 CDS 100% 13.200 9.240 N CDK12 n/a
11 TRCN0000001795 GCTCGGCTCTATAACTCTGAA pLKO.1 3199 CDS 100% 4.950 3.465 N CDK12 n/a
12 TRCN0000023037 CCAGAAGTAGAAGTCCTGCAT pLKO.1 1622 CDS 100% 2.640 1.848 N Cdk12 n/a
13 TRCN0000351012 CCAGAAGTAGAAGTCCTGCAT pLKO_005 1622 CDS 100% 2.640 1.848 N Cdk12 n/a
14 TRCN0000001796 GCTGCTTAACATCCACTCCAA pLKO.1 3966 CDS 100% 2.640 1.848 N CDK12 n/a
15 TRCN0000235471 TACCTTGTATTTGAGTATATG pLKO_005 2986 CDS 100% 0.000 0.000 N CDK12 n/a
16 TRCN0000235472 CCACCTCAGCAACCATATAAA pLKO_005 2635 CDS 100% 15.000 9.000 N CDK12 n/a
17 TRCN0000001798 GCACTGAAAGAGGAGATTGTT pLKO.1 2077 CDS 100% 5.625 3.375 N CDK12 n/a
18 TRCN0000023036 GCAGTGTTATTAAACCTGCTT pLKO.1 3907 CDS 100% 2.640 1.848 N Cdk12 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017024753.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

No results found.