Vectors

If you're unsure where to begin, Vectors for Assay Development guides you through the most common starting-point vectors to conduct your screen.
If you are a lab affiliated with the Broad Institute, these vectors are available directly from the Genetic Perturbation Platform.
Please visit the Broad Community Site to request an aliquot.
Non-Broad affiliated labs can obtain these vectors from Addgene.

Fragmid-based vectors

Fragmid is a modular toolkit for the creation of CRISPR vectors. To browse existing Fragmid-based vectors, please see dimgarF.

Fragmid

Explore your options and design your CRISPR vectors via our modular assembly tool.

dimgarF

Search and browse existing vectors made via Fragmid.

Legacy vectors

Legacy vectors are older vectors not assembled via Fragmid. While these vectors are still functional and will remain available, we suggest Fragmid-based ones to make full use of our positive controls and streamline any potential future modifications.

For legacy vectors sorted by perturbagen type, please use the following links:

• CRISPR Vector Summary
• shRNA Vector Summary
• ORF Vector Summary