Vectors
If you are unsure where to begin,
Vectors for Assay Development guides you through the most common starting-point vectors to conduct your screen.
If you are affiliated with the Broad Institute, please visit the
Broad Community Site for information on ordering vector aliquots. Non-Broad affiliates can obtain vectors through Addgene.
Fragmid-based vectors
By generating interchangeable parts comprising a variety of Cas proteins, sgRNAs, promoters, and selection markers, GPP has created a Golden Gate cloning system that enables the design of custom vectors tailored to their specific biologic application. Fragmid is an online tool that supports this system, allowing users to browse fragments and generate made-to-order vector maps, and dimgarF is a tool to browse preassembled vectors made via Fragmid. We are constantly updating available fragments and welcome ideas for ones that expand the capabilities of these resources.
For more information about the Fragmid toolkit, read our paper on modular vector assembly.
Legacy vectors
Legacy vectors are older vectors not assembled via Fragmid. While these vectors are still functional and will remain available, we suggest Fragmid-based ones to make full use of our positive controls and streamline any potential future modifications.
For legacy vectors sorted by perturbagen type, please use the following links: