Construct: shRNA TRCN0000072203

Construct Description:

Construct Type:
shRNA
Other Identifiers:
rfp_283s1c1
DNA Barcode:
CGCGTGATGAACTTCGAGGAC

Original Target:

Original Target:

Taxon:
Control
Gene:
RFP (RFP)
Gene Description:
RFP
Transcript:
Broad rfp.1 (CURRENT)
Match location:
Position 283 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000072203)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this hairpin:

Taxon Gene Symbol Description Transcript SDR Match %[?] Region Start Pos.[?] Intrinsic Score[?] Adjusted Score[?]
1 CONTROL RFP RFP RFP rfp.1 100% CDS 283 0.720 N/A
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Sequence Information

Target Sequence:
CGCGTGATGAACTTCGAGGAC
Hairpin Sequence:
5'-CCGG-CGCGTGATGAACTTCGAGGAC-CTCGAG-GTCCTCGAAGTTCATCACGCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCGTGATGAACTTCGAGGACCTCGAGGTCCTCGAAGTTCATCACGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCGTGATGAACTTCGAGGACCTCGAGGTCCTCGAAGTTCATCACGCG-3'

Other clones with same target sequence:

(none)