Construct: shRNA TRCN0000072209

Construct Description:

Construct Type:
shRNA
Other Identifiers:
rfp_188s1c1
DNA Barcode:
CTCAGTTCCAGTACGGCTCCA

Original Target:

Taxon:
Control
Gene:
RFP (RFP)
Gene Description:
RFP
Transcript:
Broad rfp.1 (CURRENT)
Match location:
Position 188 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000072209)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this hairpin:

Taxon Gene Symbol Description Transcript SDR Match %[?] Region Start Pos.[?] Intrinsic Score[?] Adjusted Score[?]
1 CONTROL RFP RFP RFP rfp.1 100% CDS 188 0.720 N/A
2 human 155184 SLC2A7 solute carrier family 2 mem... NM_207420.2 86% CDS 99 N/A N/A
3 human 155184 SLC2A7 solute carrier family 2 mem... XM_011540824.2 86% CDS 138 N/A N/A
4 human 155184 SLC2A7 solute carrier family 2 mem... XM_011540825.2 86% CDS 138 N/A N/A
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Sequence Information

Target Sequence:
CTCAGTTCCAGTACGGCTCCA
Hairpin Sequence:
5'-CCGG-CTCAGTTCCAGTACGGCTCCA-CTCGAG-TGGAGCCGTACTGGAACTGAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTCAGTTCCAGTACGGCTCCACTCGAGTGGAGCCGTACTGGAACTGAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTCAGTTCCAGTACGGCTCCACTCGAGTGGAGCCGTACTGGAACTGAG-3'

Other clones with same target sequence:

  1. TRCN0000231683