Construct: shRNA TRCN0000222803

Construct Description:

Construct Type:
shRNA
Other Identifiers:
turboGfp-170s1c1
DNA Barcode:
ACCTGCTGAGCCACGTGATGG

Original Target:

Taxon:
Control
Gene:
tGFP (tGFP)
Gene Description:
turboGfp
Transcript:
Broad turboGfp.1 (CURRENT)
Match location:
Position 170 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000222803)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACCTGCTGAGCCACGTGATGG
Hairpin Sequence:
5'-CCGG-ACCTGCTGAGCCACGTGATGG-CTCGAG-CCATCACGTGGCTCAGCAGGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACCTGCTGAGCCACGTGATGGCTCGAGCCATCACGTGGCTCAGCAGGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACCTGCTGAGCCACGTGATGGCTCGAGCCATCACGTGGCTCAGCAGGT-3'

Other clones with same target sequence:

(none)