Protocols

Cloning and Plasmid DNA (pDNA) Production

Protocol Name Date Download
1 shRNA/sgRNA Cloning Protocol 30 Sep 2015 pdf
2 shRNA/sgRNA/ORF Glycerol and Plasmid DNA Preparation 30 Sep 2015 pdf
3 pDNA Library Amplification 4 Feb 2016 pdf

Virus Production and Titering

Protocol Name Date Download
1 shRNA/sgRNA/ORF High Throughput Viral Production (96 well) 30 Sep 2015 pdf
2 shRNA/sgRNA/ORF Low Throughput Viral Production (10cm dish/6 well) 3 Jun 2015 pdf
3 shRNA/sgRNA/ORF Large Scale Lentiviral Production (T-175 flasks) 2 May 2016 pdf
4 Viral Titering Protocol (alamarBlue) 21 Sep 2016 pdf

Cas9 Activity Assessment

Protocol Name Date Download
1 Cas9 Activity Assay 11 Mar 2016 pdf
2 Out-of-frame eGFP CRISPR Gene Editing Reporter Assay 30 Sep 2015 pdf

Viral Infection

Protocol Name Date Download
1 Introducing Cas9 Lentivirus 30 Sep 2015 pdf
2 No-Spin Infection Protocol 21 Jun 2016 zip

gDNA Isolation, PCR and Next-Generation Sequence Data Analysis

Protocol Name Date Download
1 gDNA Miniprep Isolation Protocol 1 Sep 2016 pdf
2 gDNA Midi/Maxiprep Isolation Protocol 21 Jan 2016 pdf
3 Alternate gDNA Maxiprep Isolation Protocol (NucleoSpin Blood XL-Maxi Kit) 25 Feb 2016 pdf
4 sgRNA/shRNA/ORF PCR for Illumina Sequencing 26 Oct 2015 pdf
5 Pooled Screening Deconvolution Using PoolQ 2 Mar 2016 pdf
6 Introduction to Pooled Screen Analysis 17 Apr 2017 pdf