Protocols

Reagent Production and QC

Protocol Name Date Download
1 Mycoplasma Detection Protocol 25 Feb 2016 pdf
2 shRNA/sgRNA Cloning Protocol 30 Sep 2015 pdf
3 shRNA/sgRNA/ORF Glycerol and Plasmid DNA Preparation 30 Sep 2015 pdf
4 pDNA Library Amplification 4 Feb 2016 pdf
5 shRNA/sgRNA/ORF High Throughput Viral Production (96 well) 30 Sep 2015 pdf
6 shRNA/sgRNA/ORF Low Throughput Viral Production (10cm dish/6 well) 3 Jun 2015 pdf
7 shRNA/sgRNA/ORF Large Scale Lentiviral Production (T-175 flasks) 2 May 2016 pdf
8 No-Spin Infection Protocol 21 Jun 2016 zip
9 Viral Titering Protocol (alamarBlue) 21 Sep 2016 pdf

Cas9 Delivery and Activity Assessment

Protocol Name Date Download
1 Introducing Cas9 Lentivirus 30 Sep 2015 pdf
2 Out-of-frame eGFP CRISPR Gene Editing Reporter Assay 30 Sep 2015 pdf
3 Cas9 Activity Assay 11 Mar 2016 pdf

Screening Experimentation and Analysis

Protocol Name Date Download
1 gDNA Miniprep Isolation Protocol 1 Sep 2016 pdf
2 gDNA Midi/Maxiprep Isolation Protocol 21 Jan 2016 pdf
3 Alternate gDNA Maxiprep Isolation Protocol (NucleoSpin Blood XL-Maxi Kit) 25 Feb 2016 pdf
4 sgRNA/shRNA/ORF PCR for Illumina Sequencing 26 Oct 2015 pdf
5 Pooled Screening Deconvolution Using PoolQ 2 Mar 2016 pdf