Construct: sgRNA BRDN0001144724

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TGTGCATGATTCCCTTGCTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CSNK2A2 (1459)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75586

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000016.10 16 58168674 + CSNK2A2 NNNGCATGATTCCCTTGCTG NGG 0 1.0 Tier I
2 NC_000005.10 5 6378093 + MED10 NNNGCATGATTCCCTTGATA NGG 2 0.5048 Tier II
3 NC_000017.11 17 58301350 - TSPOAP1 NNNGCATGTTTCCTTTGCTG NGG 2 0.44 Tier II
4 NC_000011.10 11 2714820 - KCNQ1 NNNGCATCATTCCCTTGCTT NGG 2 0.4308 Tier II
5 NC_000001.11 1 14964232 + KAZN NNNCCATGATTCCTTTGCTG NGG 2 0.3882 Tier II
6 NC_000011.10 11 132198252 + NTM NNNCCATGATGCCCTTGCTG NGG 2 0.2118 Tier II
7 NC_000005.10 5 114212322 + KCNN2 NNNGCATAATTCCCTTGGTG NGG 2 0.1333 Tier II
8 NC_000005.10 5 170022576 + DOCK2 NNNACATGATTCCCTTGGTG NGG 2 0.12 Tier II
9 NC_000012.12 12 103773146 - NT5DC3 NNNGCAAGATTCCCTTGCTG NGA 2 0.0608 Tier II
10 NC_000001.11 1 44264522 - ERI3 NNNGCATGATTACCTTGCTG NGA 2 0.0496 Tier II
11 NC_000011.10 11 33515589 - KIAA1549L NNNTCATGATTCCCTTGCTG NCG 2 0.039 Tier II
12 NC_000006.12 6 5990992 - LOC105374898 NNNGAATGAATCCCTTGCTG NGG 2 0.4898 Tier III
13 NC_000017.11 17 81343109 + RENO1 NNNGCATGATACCCTGGCTG NGG 2 0.2596 Tier III
14 NC_000012.12 12 67541106 - LINC02408 NNNGCATGATTCCCTTTCTA NGG 2 0.2344 Tier III
15 NC_000021.9 21 41640719 + LOC105372812 NNNGAATGATGCCCTTGCTG NGG 2 0.2286 Tier III
16 NC_000009.12 9 40952217 - LOC107986997 NNNGCATGATCCCCCTGCTG NGG 2 0.1818 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000074.6 8 95461610 + Csnk2a2 NNNGCATGATTCCCTTGCTG NGG 0 1.0 Tier I
2 NC_000072.6 6 70893114 - Eif2ak3 NNNGCATGAGTCCCTTGCTG NGC 2 0.0111 Tier I
3 NC_000071.6 5 119824969 + Tbx5 NNNGCTTGATTCCCTTGCTG NGG 1 0.7143 Tier II
4 NC_000074.6 8 118226711 - Cdh13 NNNGCATGATTCCTTTGCTA NGG 2 0.6875 Tier II
5 NC_000076.6 10 87885130 - Igf1 NNNGCAGAATTCCCTTGCTG NGG 2 0.5882 Tier II
6 NC_000077.6 11 36457438 - Tenm2 NNNGAATGATTCCCTTGTTG NGG 2 0.3673 Tier II
7 NC_000077.6 11 21644437 + Wdpcp NNNGCATGAGTCCTTTGCTG NGG 2 0.3667 Tier II
8 NC_000086.7 X 168275220 - Frmpd4 NNNCCATGATTTCCTTGCTG NGG 2 0.2851 Tier II
9 NC_000076.6 10 120017798 + Grip1 NNNTCATGATTCCCTTGTTG NGG 2 0.2338 Tier II
10 NC_000079.6 13 30204716 + Mboat1 NNNGTATGATTCCATTGCTG NGG 2 0.2227 Tier II
11 NC_000079.6 13 48605803 - Ptpdc1 NNNGCATCATTCCATTGCTG NGG 2 0.2154 Tier II
12 NC_000077.6 11 52866390 - Fstl4 NNNGCATCATTCCCTGGCTG NGG 2 0.213 Tier II
13 NC_000082.6 16 4660380 + Coro7 NNNGCATGATTCTCTTGCAG NGG 2 0.2 Tier II
14 NC_000067.6 1 106559386 - Bcl2 NNNGCATGATTGCATTGCTG NGG 2 0.1556 Tier II
15 NC_000069.6 3 145376952 + Col24a1 NNNGCATGTTTCCCTTGCTG NAG 2 0.1556 Tier II
16 NC_000086.7 X 37876373 + Rhox8 NNNGCATCATTCCCTTTCTG NGG 2 0.1538 Tier II
17 NC_000070.6 4 117634281 + Eri3 NNNGCATGATTACCTTGCTG NGA 2 0.0496 Tier II
18 NC_000073.6 7 136953823 + Mgmt NNNGCATGATTCCCTGGCTG NGA 2 0.024 Tier II
19 NC_000084.6 18 62184537 + Gm9949 NNNGCATGATTCTCTTGCTG NGT 2 0.0113 Tier II
20 NC_000069.6 3 127365981 - Ank2 NNNGCATGATGCCCTTGCTG NGT 2 0.0065 Tier II
21 NC_000071.6 5 119824969 + Gm5563 NNNGCTTGATTCCCTTGCTG NGG 1 0.7143 Tier III
22 NC_000071.6 5 138894486 - Gm31891 NNNGCATGATTGCCTTGTTG NGG 2 0.2857 Tier III
23 NC_000068.7 2 16980560 - 4930515L03Rik NNNCCATGATTTCCTTGCTG NGG 2 0.2851 Tier III
24 NC_000069.6 3 98351585 + Gm31420 NNNGCATGCTTGCCTTGCTG NGG 2 0.254 Tier III
25 NC_000078.6 12 43735701 - Gm33384 NNNACATGATTCCCTTTCTG NGG 2 0.225 Tier III
26 NC_000078.6 12 29007193 - Gm31508 NNNGCATCATTCCCTGGCTG NGG 2 0.213 Tier III
27 NC_000074.6 8 50297366 - 1700019L22Rik NNNTCATGATTCCCTTGCTG NGA 2 0.0253 Tier III
28 NC_000080.6 14 104896821 - D130009I18Rik NNNGCATGATTCCCTTCCTG NTG 2 0.0092 Tier III
29 NC_000067.6 1 152221316 + Gm36920 NNNGCATGATTCACTTGCTG NGC 2 0.0085 Tier III
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Other clones with same target sequence:

(none)