Construct: sgRNA BRDN0001144736

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TAGTGGATTTATGGTCTGTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MAPK8 (5599)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
78046

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000010.11 10 48424102 + MAPK8 NNNTGGATTTATGGTCTGTG NGG 0 1.0 Tier I
2 NC_000004.12 4 86098582 - MAPK10 NNNTGGATATATGGTCTGTG NGA 2 0.0645 Tier I
3 NC_000002.12 2 42567458 - MTA3 NNNTGGGTTTAAGGTCTGTG NGG 2 0.5647 Tier II
4 NC_000018.10 18 79806954 + KCNG2 NNNTGGATCTAGGGTCTGTG NGG 2 0.4615 Tier II
5 NC_000002.12 2 141325889 + LRP1B NNNTGGATTGATAGTCTGTG NGG 2 0.4615 Tier II
6 NC_000020.11 20 1171504 - PSMF1 NNNAGGATTTGTGGTCTGTG NGG 2 0.3095 Tier II
7 NC_000017.11 17 15898792 + ADORA2B NNNTGGATGGATGGTCTGTG NGG 2 0.3095 Tier II
8 NC_000023.11 X 11354586 - ARHGAP6 NNNTGGATATATGGTTTGTG NGG 2 0.2857 Tier II
9 NC_000017.11 17 164591 - DOC2B NNNTGGATTTAGGGTCAGTG NGG 2 0.2667 Tier II
10 NC_000002.12 2 233552100 + USP40 NNNTGGATTATTGGTCTGTG NGG 2 0.2637 Tier II
11 NC_000008.11 8 81104571 - PAG1 NNNTGGATTTATGCACTGTG NGG 2 0.2481 Tier II
12 NC_000010.11 10 24404851 + KIAA1217 NNNTGGATTTATGCTCAGTG NGG 2 0.2286 Tier II
13 NC_000002.12 2 120953176 - GLI2 NNNTGGATTTAGGGCCTGTG NGG 2 0.1364 Tier II
14 NC_000001.11 1 163208684 - RGS5 NNNTGGATTTATTGTCTGTC NGG 2 0.1286 Tier II
15 NC_000012.12 12 57696481 - OS9 NNNTGGATTTAGGGTCTGGG NGG 2 0.125 Tier II
16 NC_000012.12 12 12179268 - LRP6 NNNTTGATTTATGGGCTGTG NGG 2 0.015 Tier II
17 NC_000013.11 13 114219794 + CFAP97D2 NNNTGGAATTATGGTCTGTG NGT 2 0.0129 Tier II
18 NC_000003.12 3 111550822 + CD96 NNNTGGATTTTTGGTCTGTG NTG 2 0.012 Tier II
19 NC_000016.10 16 7579509 + RBFOX1 NNNTGGATTTATTGTCTGTG NTG 2 0.0117 Tier II
20 NC_000002.12 2 43391985 - THADA NNNTGGATTTCTGGTCTGTG NGC 2 0.0089 Tier II
21 NC_000021.9 21 14843335 - ASMER1 NNNAGGGTTTATGGTCTGTG NGG 2 0.3361 Tier III
22 NC_000012.12 12 115879280 - LINC02463 NNNAGGATTTATGGTCTGTC NGG 2 0.2041 Tier III
23 NC_000001.11 1 163208684 - RGS5-AS1 NNNTGGATTTATTGTCTGTC NGG 2 0.1286 Tier III
24 NC_000009.12 9 38362470 + LOC105376040 NNNTGGATTAATGGTCTGTG NGC 2 0.019 Tier III
25 NC_000009.12 9 38362470 + LOC107987065 NNNTGGATTAATGGTCTGTG NGC 2 0.019 Tier III
26 NC_000008.11 8 95455647 + CFAP418-AS1 NNNTGGGTTTATGGTCTGTG NGC 2 0.0157 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000080.6 14 33390731 - Mapk8 NNNTGGACTTATGGTCTGTG NGG 1 1.0 Tier I
2 NC_000070.6 4 144377228 - Pramel15 NNNTGGATTTACGGTCTGTG NGA 2 0.0658 Tier I
3 NC_000075.6 9 83956796 + Bckdhb NNNTGGATTAATGATCTGTG NGG 2 0.6429 Tier II
4 NC_000081.6 15 73387045 - Ptk2 NNNTGGATTTATAGTCTTTG NGG 2 0.6154 Tier II
5 NC_000073.6 7 75715952 - Akap13 NNNTGGACTTATGGACTGTG NGG 2 0.5789 Tier II
6 NC_000077.6 11 4073252 - Sec14l3 NNNTATATTTATGGTCTGTG NGG 2 0.5778 Tier II
7 NC_000075.6 9 10754144 - Cntn5 NNNTGACTTTATGGTCTGTG NGG 2 0.4375 Tier II
8 NC_000077.6 11 116374441 - Rnf157 NNNTGTATTTGTGGTCTGTG NGG 2 0.4333 Tier II
9 NC_000085.6 19 42601885 - Loxl4 NNNTGAATTTATCGTCTGTG NGG 2 0.4211 Tier II
10 NC_000072.6 6 144771858 + Sox5 NNNAGGATTTAAGGTCTGTG NGG 2 0.381 Tier II
11 NC_000070.6 4 138623857 - Vwa5b1 NNNTGGGTTTATGGTCTGTC NGG 2 0.3025 Tier II
12 NC_000068.7 2 59026161 - Ccdc148 NNNTGGATTTCTGGTCTGTT NGG 2 0.28 Tier II
13 NC_000083.6 17 11875574 - Prkn NNNTGGTTTTATGGACTGTG NGG 2 0.2533 Tier II
14 NC_000085.6 19 18824829 + Trpm6 NNNTTGAGTTATGGTCTGTG NGG 2 0.22 Tier II
15 NC_000078.6 12 101868597 + Trip11 NNNGGGATTTTTGGTCTGTG NGG 2 0.1923 Tier II
16 NC_000083.6 17 46516518 + Cul9 NNNTGGATGTATGGCCTGTG NGG 2 0.1688 Tier II
17 NC_000080.6 14 118844105 - Cldn10 NNNTTGATTTAGGGTCTGTG NGG 2 0.15 Tier II
18 NC_000068.7 2 124157558 - Sema6d NNNTGGATTTATGCTTTGTG NGG 2 0.1319 Tier II
19 NC_000069.6 3 130235792 - Col25a1 NNNTGGTTTTATGGTCTGAG NGG 2 0.125 Tier II
20 NC_000069.6 3 152555609 - Ak5 NNNTGTATTTATGGTGTGTG NGG 2 0.1026 Tier II
21 NC_000075.6 9 78412691 + Ddx43 NNNTGGATTTGTGGTGTGTG NGG 2 0.1 Tier II
22 NC_000083.6 17 19366347 - Vmn2r99 NNNTGGATTTATGTTCTGAG NGG 2 0.0762 Tier II
23 NC_000081.6 15 35816199 - Vps13b NNNTGGATTTAAGGTCTGTG NGT 2 0.0129 Tier II
24 NC_000082.6 16 28895081 + Mb21d2 NNNTGGATTTATGGTCTGTC NGC 2 0.0095 Tier II
25 NC_000069.6 3 127184506 + Ank2 NNNTGGATTTATGGTCTGTG NAT 2 0.0 Tier II
26 NC_000077.6 11 79309296 - Gm30561 NNNTGTATTTATGGTATGTG NGG 2 0.6667 Tier III
27 NC_000080.6 14 55442176 + Gm52145 NNNTGTATTTATGGTATGTG NGG 2 0.6667 Tier III
28 NC_000078.6 12 71634274 + 9630002D21Rik NNNTGCTTTTATGGTCTGTG NGG 2 0.2983 Tier III
29 NC_000083.6 17 11875574 - D17Ertd648e NNNTGGTTTTATGGACTGTG NGG 2 0.2533 Tier III
30 NC_000067.6 1 105432085 - Gm28403 NNNTGGTTTTATGTTCTGTG NGG 2 0.1167 Tier III
31 NC_000079.6 13 113478488 - 4921509O07Rik NNNTGGATTTATGTGCTGTG NGG 2 0.0133 Tier III
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Other clones with same target sequence:

(none)