Construct: sgRNA BRDN0001144737
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CAGTGGCCGTCACCACATAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MAP3K6 (9064)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76013
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000001.11 |
1 |
27364363 |
+ |
MAP3K6 |
NNNTGGCCGTCACCACATAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000006.12 |
6 |
159907318 |
- |
MAS1 |
NNNTGGCCGTCAGCAGATAG |
NGG |
2 |
0.021 |
Tier I |
| 3 |
NC_000016.10 |
16 |
22257844 |
- |
EEF2K |
NNNTGGCTGTCACCACACAG |
NGG |
2 |
0.375 |
Tier II |
| 4 |
NC_000023.11 |
X |
138675879 |
- |
FGF13 |
NNNTGGCTGTCACCACATGG |
NGG |
2 |
0.3281 |
Tier II |
| 5 |
NC_000019.10 |
19 |
38045067 |
+ |
SIPA1L3 |
NNNTGGCCCTCACCACCTAG |
NGG |
2 |
0.095 |
Tier II |
| 6 |
NC_000004.12 |
4 |
72355699 |
+ |
ADAMTS3 |
NNNTGGCCTTCACCAGATAG |
NGG |
2 |
0.0821 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000077.6 |
11 |
6324462 |
+ |
Ogdh |
NNNTGGCTTTCACCACATAG |
NGG |
2 |
0.4667 |
Tier II |
| 2 |
NC_000068.7 |
2 |
146262314 |
+ |
Ralgapa2 |
NNNTGGCTGTCACCACAGAG |
NGG |
2 |
0.2917 |
Tier II |
| 3 |
NC_000070.6 |
4 |
132057966 |
+ |
Epb41 |
NNNTGGCAGTCACCACACAG |
NGG |
2 |
0.2786 |
Tier II |
| 4 |
NC_000069.6 |
3 |
13593236 |
- |
Ralyl |
NNNTGGCCATCACCACACAG |
NGG |
2 |
0.2755 |
Tier II |
| 5 |
NC_000076.6 |
10 |
106618830 |
- |
Ppfia2 |
NNNTGGCCATCACCACATAG |
NAG |
2 |
0.1667 |
Tier II |
| 6 |
NC_000083.6 |
17 |
50612273 |
- |
Plcl2 |
NNNTGGCCGTGACCTCATAG |
NGG |
2 |
0.05 |
Tier II |
| 7 |
NC_000076.6 |
10 |
39893423 |
- |
Mfsd4b4 |
NNNTGGCCGTCACCTCATAG |
NGT |
2 |
0.0032 |
Tier II |
| 8 |
NC_000068.7 |
2 |
146262314 |
+ |
A930019D19Rik |
NNNTGGCTGTCACCACAGAG |
NGG |
2 |
0.2917 |
Tier III |
Other clones with same target sequence:
(none)
