Construct: sgRNA BRDN0001144760

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GATAGCAAGTGATCCACACT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
STK31 (56164)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77376

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000007.14 7 23772189 - STK31 NNNAGCAAGTGATCCACACT NGG 0 1.0 Tier I
2 NC_000019.10 19 41716659 + CEACAM5 NNNAGCAAGTGACCCACACT NGG 1 0.7895 Tier II
3 NC_000019.10 19 42520227 - CEACAM1 NNNAGCGAGTGACCCACACT NGG 2 0.5573 Tier II
4 NC_000004.12 4 2343174 - ZFYVE28 NNNAGGAAGTAATCCACACT NGG 2 0.5 Tier II
5 NC_000004.12 4 92341329 - GRID2 NNNAGCAAGTGATCCACATT NGG 1 0.4615 Tier II
6 NC_000010.11 10 120567016 - PLPP4 NNNAGCCAGAGATCCACACT NGG 2 0.375 Tier II
7 NC_000020.11 20 47730922 + SULF2 NNNAGCAGGTGATCCACGCT NGG 2 0.2933 Tier II
8 NC_000011.10 11 19567268 + NAV2 NNNGGCCAGTGATCCACACT NGG 2 0.2734 Tier II
9 NC_000003.12 3 37565834 - ITGA9 NNNAGCAAGGGTTCCACACT NGG 2 0.1667 Tier II
10 NC_000009.12 9 16630009 + BNC2 NNNAGCATGTTATCCACACT NGG 2 0.1648 Tier II
11 NC_000016.10 16 73180636 - ZFHX3 NNNAGCAAGTGATACACACT NAG 2 0.0907 Tier II
12 NC_000012.12 12 104025889 + GLT8D2 NNNAGCAAGTGATCCACACA NGT 2 0.0091 Tier II
13 NC_000007.14 7 140041520 + PARP12 NNNAGCAAGTGAGCCACACT NGC 2 0.0058 Tier II
14 NC_000019.10 19 41762999 + CEACAM6 NNNAGCAAGTGACGCACACT NGG 2 0.0 Tier II
15 NC_000010.11 10 69941661 + COL13A1 NNNAGCAAGTGAGGCACACT NGG 2 0.0 Tier II
16 NC_000018.10 18 80173216 + PARD6G NNNAGCATGTGATGCACACT NGG 2 0.0 Tier II
17 NC_000019.10 19 42520227 - LIPE-AS1 NNNAGCGAGTGACCCACACT NGG 2 0.5573 Tier III
18 NC_000004.12 4 2343174 - LOC105374352 NNNAGGAAGTAATCCACACT NGG 2 0.5 Tier III
19 NC_000004.12 4 2343174 - LOC124900649 NNNAGGAAGTAATCCACACT NGG 2 0.5 Tier III
20 NC_000005.10 5 163099136 + LOC105377700 NNNAGCAAGTGATCCAAACT NAG 2 0.121 Tier III
21 NC_000007.14 7 66860140 - GTF2IP23 NNNAGCAAGTGATCCACAGA NGG 2 0.0703 Tier III
22 NC_000018.10 18 80173216 + PARD6G-AS1 NNNAGCATGTGATGCACACT NGG 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000072.6 6 49439141 - Stk31 NNNAGCAAGTAATCCACATT NGG 2 0.4615 Tier I
2 NC_000077.6 11 76190891 + Tlcd3a NNNACCAAGTGATTCACACT NGG 2 0.5762 Tier II
3 NC_000067.6 1 172348391 + Kcnj10 NNNAGCAAGTGACCCATACT NGG 2 0.3684 Tier II
4 NC_000073.6 7 66013791 - Pcsk6 NNNAGCAAGAGTTCCACACT NGG 2 0.2857 Tier II
5 NC_000075.6 9 41026741 - Ubash3b NNNAGGAAGTGATCCACACA NGG 2 0.2813 Tier II
6 NC_000081.6 15 80196685 + Mgat3 NNNAGCAAGTGAGCCACACT NGG 1 0.2609 Tier II
7 NC_000069.6 3 146656223 + 4930503B20Rik NNNAGCAAATGTTCCACACT NGG 2 0.2143 Tier II
8 NC_000083.6 17 67746234 - Lama1 NNNAGCAATTGATACACACT NGG 2 0.1867 Tier II
9 NC_000076.6 10 7163163 - Cnksr3 NNNAGCATGTGAGCCACACT NGG 2 0.1118 Tier II
10 NC_000079.6 13 24964115 + Gpld1 NNNAGCAAGTGTTCCACCCT NGG 2 0.0635 Tier II
11 NC_000069.6 3 157569924 + Ptger3 NNNAGCAAGTGATCCAAACT NCG 2 0.05 Tier II
12 NC_000078.6 12 53791086 - Npas3 NNNAGCAACTGATCCACACC NGG 2 0.049 Tier II
13 NC_000080.6 14 56555337 - Cenpj NNNGGCAAGTGATCTACACT NGG 2 0.0417 Tier II
14 NC_000069.6 3 123079582 + Synpo2 NNNAGGAAGTGATCCACACT NGT 2 0.0081 Tier II
15 NC_000068.7 2 119193662 + Dnajc17 NNNAGCAAGTGAACCTCACT NGG 2 0.0 Tier II
16 NC_000077.6 11 68042687 + Stx8 NNNAGCAAGTGATCCTCTCT NGG 2 0.0 Tier II
17 NC_000073.6 7 10805716 + Gm18781 NNNAGAATGTGATCCACACT NGG 2 0.398 Tier III
18 NC_000076.6 10 69312192 + Gm40685 NNNAGCAAGTGTTCCTCACT NGG 2 0.0 Tier III
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Other clones with same target sequence:

(none)