Construct: sgRNA BRDN0001144777

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
CCGCAGCTCAGGAATATAGG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CDC42BPG (55561)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77528

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000011.10 11 64838725 + CDC42BPG NNNCAGCTCAGGAATATAGG NGG 0 1.0 Tier I
2 NC_000005.10 5 45293561 + HCN1 NNNTAGCACAGGAATATAGG NGG 2 0.64 Tier II
3 NC_000001.11 1 76343386 + ST6GALNAC3 NNNCAGTTCAGGAATATAAG NGG 2 0.5804 Tier II
4 NC_000009.12 9 113955544 - ZNF618 NNNCAGCCCAGGAAAATAGG NGG 2 0.5789 Tier II
5 NC_000002.12 2 165554226 - CSRNP3 NNNCAGCACAGGAATATAAG NGG 2 0.5714 Tier II
6 NC_000001.11 1 24788764 - CLIC4 NNNCAAGTCAGGAATATAGG NGG 2 0.4706 Tier II
7 NC_000006.12 6 130223523 - SAMD3 NNNCAGCTGAGCAATATAGG NGG 2 0.3277 Tier II
8 NC_000002.12 2 232620930 + EFHD1 NNNCTGCTTAGGAATATAGG NGG 2 0.3182 Tier II
9 NC_000017.11 17 77409122 - SEPTIN9 NNNCAGCTCAGGAAAATGGG NGG 2 0.2316 Tier II
10 NC_000011.10 11 64114937 + FLRT1 NNNCAGCTCAGGAATAGAGA NGG 2 0.1103 Tier II
11 NC_000011.10 11 64114937 + MACROD1 NNNCAGCTCAGGAATAGAGA NGG 2 0.1103 Tier II
12 NC_000007.14 7 139963820 + TBXAS1 NNNCAGCTCAGGAATAGATG NGG 2 0.0784 Tier II
13 NC_000012.12 12 11804983 - ETV6 NNNCAGCTCTGGAATATAGG NGA 2 0.0613 Tier II
14 NC_000006.12 6 42030075 + CCND3 NNNCAGCTCAGGAATACAGG NGT 2 0.0114 Tier II
15 NC_000015.10 15 42562060 - HAUS2 NNNCAGCTCAGGAATACAGG NGT 2 0.0114 Tier II
16 NC_000001.11 1 69643144 + LRRC7 NNNCAGCTCAGGAAGATAGG NGA 2 0.0035 Tier II
17 NC_000017.11 17 1621327 - SLC43A2 NNNCAGCTCAGGAATTTAGA NGG 2 0.0 Tier II
18 NC_000006.12 6 156285457 + LOC101928923 NNNTATCTCAGGAATATAGG NGG 2 0.5333 Tier III
19 NC_000013.11 13 44147055 + SMIM2 NNNCAGCTCAGGAAAATGGG NGG 2 0.2316 Tier III
20 NC_000013.11 13 44147055 + SMIM2-IT1 NNNCAGCTCAGGAAAATGGG NGG 2 0.2316 Tier III
21 NC_000013.11 13 44147055 + SMIM2-AS1 NNNCAGCTCAGGAAAATGGG NGG 2 0.2316 Tier III
22 NC_000013.11 13 44147055 + LINC00390 NNNCAGCTCAGGAAAATGGG NGG 2 0.2316 Tier III
23 NC_000017.11 17 72523937 + LINC00673 NNNCTGGTCAGGAATATAGG NGG 2 0.1711 Tier III
24 NC_000012.12 12 27152133 - LOC124902904 NNNCAGCTCCCGAATATAGG NGG 2 0.1429 Tier III
25 NC_000007.14 7 34521815 - NPSR1-AS1 NNNCAGCTCAGGAGCATAGG NGG 2 0.1273 Tier III
26 NC_000003.12 3 66933305 + LOC105377144 NNNAAGCTCAGGAATATAGG NGA 2 0.0585 Tier III
27 NC_000005.10 5 89128339 - MEF2C-AS1 NNNCAGCTCAGGCAGATAGG NGG 2 0.0105 Tier III
28 NC_000021.9 21 37531075 + KCNJ6-AS1 NNNCAGCTCAGGAATCTAGG NGG 1 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000075.6 9 7994096 + Yap1 NNNAAGCCCAGGAATATAGG NGG 2 0.8421 Tier II
2 NC_000072.6 6 67458597 - Il23r NNNCAGCTCAAGAATATATG NGG 2 0.6667 Tier II
3 NC_000070.6 4 86129059 - Adamtsl1 NNNCAGATTAGGAATATAGG NGG 2 0.6563 Tier II
4 NC_000085.6 19 55074021 - Gpam NNNCAACTCAGGAATATTGG NGG 2 0.5 Tier II
5 NC_000073.6 7 63485811 + Otud7a NNNCAGCTCAGGAATATTGG NGG 1 0.5 Tier II
6 NC_000074.6 8 113832595 + Adamts18 NNNGAGCCCAGGAATATAGG NGG 2 0.5 Tier II
7 NC_000078.6 12 79434728 + Rad51b NNNCAGCTCAGGAATATATT NGG 2 0.4667 Tier II
8 NC_000077.6 11 110511939 + Map2k6 NNNCAGCTAAGCAATATAGG NGG 2 0.4538 Tier II
9 NC_000071.6 5 8807943 - Abcb1b NNNCAACTCAGGAATATGGG NGG 2 0.4 Tier II
10 NC_000080.6 14 79414180 + Mtrf1 NNNTAGCTCAGGAATATTGG NGG 2 0.4 Tier II
11 NC_000081.6 15 76445548 - Mroh1 NNNCAGCTCAGAAATATGGG NGG 2 0.3733 Tier II
12 NC_000074.6 8 84388248 - Cacna1a NNNCAGCTCATGAATATAGT NGG 2 0.2692 Tier II
13 NC_000077.6 11 29417225 + Ccdc88a NNNCAGCTCAGGAACATAGA NGG 2 0.2557 Tier II
14 NC_000083.6 17 47771943 + Tfeb NNNCAGCTCAGGAAGATAGA NGG 2 0.0469 Tier II
15 NC_000071.6 5 136293315 + Cux1 NNNCAGCTAAGGAAGATAGG NGG 2 0.0429 Tier II
16 NC_000083.6 17 67305458 - Ptprm NNNCAGCTCAGGTAGATAGG NGG 2 0.015 Tier II
17 NC_000078.6 12 30289982 - Sntg2 NNNCAGCACAGGAATATAGG NGT 2 0.0129 Tier II
18 NC_000079.6 13 107497542 - Gm31803 NNNCAGCCCAGGAATATAGG NAG 2 0.2593 Tier III
19 NC_000071.6 5 39273593 + Gm40293 NNNCAGCTCAGGAATATGGC NGG 2 0.1714 Tier III
20 NC_000069.6 3 155996853 + 9330178D15Rik NNNCAGCTCAGGAATATAGA NGT 2 0.0151 Tier III
21 NC_000079.6 13 50325296 - Gm46385 NNNCAGCTCAGGAAACTAGG NGG 2 0.0 Tier III
22 NC_000079.6 13 50762776 + 4930518P08Rik NNNCAGCTCAGGAAACTAGG NGG 2 0.0 Tier III
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Other clones with same target sequence:

(none)