Construct: sgRNA BRDN0001144860
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GTGACTTGCCTGATCAAACT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- STK31 (56164)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77374
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000007.14 |
7 |
23729242 |
- |
STK31 |
NNNACTTGCCTGATCAAACT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000005.10 |
5 |
54549905 |
- |
SNX18 |
NNNAGTTGCCTAATCAAACT |
NGG |
2 |
0.56 |
Tier II |
| 3 |
NC_000011.10 |
11 |
67069143 |
- |
RHOD |
NNNACTTGCCTGATAAAAAT |
NGG |
2 |
0.0952 |
Tier II |
| 4 |
NC_000003.12 |
3 |
140196445 |
+ |
CLSTN2 |
NNNACTTGCCTGATCAAACC |
NGG |
1 |
0.0909 |
Tier II |
| 5 |
NC_000013.11 |
13 |
78621060 |
+ |
OBI1 |
NNNACTTGCCTCATCAAACT |
NTG |
2 |
0.0206 |
Tier II |
| 6 |
NC_000004.12 |
4 |
21663035 |
- |
KCNIP4 |
NNNACTTGCCTGATCAAAAT |
NTG |
2 |
0.0167 |
Tier II |
| 7 |
NC_000012.12 |
12 |
2311757 |
+ |
CACNA1C |
NNNACTTGCCTGATAAAACT |
NGA |
2 |
0.0154 |
Tier II |
| 8 |
NC_000005.10 |
5 |
114124121 |
- |
KCNN2 |
NNNACTTGGCTGATCAAACT |
NGC |
2 |
0.0138 |
Tier II |
| 9 |
NC_000011.10 |
11 |
132038768 |
- |
NTM |
NNNACTTGCCTGATTAAAGT |
NGG |
2 |
0.0083 |
Tier II |
| 10 |
NC_000002.12 |
2 |
231119448 |
+ |
HTR2B |
NNNACTTGCCTGATCCAACA |
NGG |
2 |
0.0 |
Tier II |
| 11 |
NC_000002.12 |
2 |
231119448 |
+ |
PSMD1 |
NNNACTTGCCTGATCCAACA |
NGG |
2 |
0.0 |
Tier II |
| 12 |
NC_000003.12 |
3 |
10550985 |
- |
ATP2B2 |
NNNACTTGCCTGATCCAAGT |
NGG |
2 |
0.0 |
Tier II |
| 13 |
NC_000003.12 |
3 |
55607933 |
+ |
ERC2 |
NNNACTTGCCTGATGCAACT |
NGG |
2 |
0.0 |
Tier II |
| 14 |
NC_000012.12 |
12 |
118899743 |
+ |
LOC105370019 |
NNNACTAGCCTGATCAAACT |
NGG |
1 |
0.875 |
Tier III |
| 15 |
NC_000012.12 |
12 |
114432342 |
- |
LOC105369998 |
NNNACTTGCCTGAACAAATT |
NGG |
2 |
0.2857 |
Tier III |
| 16 |
NC_000002.12 |
2 |
156039808 |
+ |
LINC01876 |
NNNACTTGCCTTATCAATCT |
NGG |
2 |
0.1923 |
Tier III |
| 17 |
NC_000023.11 |
X |
75731910 |
+ |
LOC107985664 |
NNNACTTGCCTTATCAATCT |
NGG |
2 |
0.1923 |
Tier III |
| 18 |
NC_000011.10 |
11 |
34762509 |
+ |
LOC102723568 |
NNNACTTGCCTTATGAAACT |
NGG |
2 |
0.0192 |
Tier III |
| 19 |
NC_000003.12 |
3 |
55607933 |
+ |
LOC105377100 |
NNNACTTGCCTGATGCAACT |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000072.6 |
6 |
49409204 |
- |
Stk31 |
NNNACTTACCTGATCAAACT |
NGG |
1 |
1.0 |
Tier I |
| 2 |
NC_000081.6 |
15 |
89475333 |
+ |
Arsa |
NNNACATGCCTGATCGAACT |
NGG |
2 |
0.1667 |
Tier I |
| 3 |
NC_000079.6 |
13 |
44881538 |
- |
Jarid2 |
NNNACTTGCATTATCAAACT |
NGG |
2 |
0.3333 |
Tier II |
| 4 |
NC_000078.6 |
12 |
56790176 |
- |
Slc25a21 |
NNNACTTGCCTGATAAAACT |
NGG |
1 |
0.2222 |
Tier II |
| 5 |
NC_000086.7 |
X |
84048191 |
- |
Dmd |
NNNACTTTCCTGATAAAACT |
NGG |
2 |
0.1389 |
Tier II |
| 6 |
NC_000081.6 |
15 |
12488125 |
- |
Pdzd2 |
NNNACTTGCCTGACCAAACT |
NTG |
2 |
0.0111 |
Tier II |
| 7 |
NC_000072.6 |
6 |
17284297 |
+ |
Cav2 |
NNNACTTGCTTGAGCAAACT |
NGG |
2 |
0.0 |
Tier II |
Other clones with same target sequence:
(none)
