Construct: sgRNA BRDN0001144878

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TTGCTTCAACCAGAACAGAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SH3BP5 (9467)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76845

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000003.12 3 15304209 + SH3BP5 NNNCTTCAACCAGAACAGAG NGG 0 1.0 Tier I
2 NC_000022.11 22 45361992 + SMC1B NNNCTTCAGCCTGAACAGAG NGG 2 0.2222 Tier I
3 NC_000011.10 11 5632281 + TRIM6-TRIM34 NNNCTTTAACCAGAAGAGAG NGG 2 0.125 Tier I
4 NC_000019.10 19 53807662 - NLRP12 NNNCTTCAAGCAGACCAGAG NGG 2 0.1061 Tier I
5 NC_000004.12 4 122876346 + FGF2 NNNCTTCAAGCAGAAGAGAG NGG 2 0.0598 Tier I
6 NC_000010.11 10 85757934 + GRID1 NNNCTCCAACCAGAACAGAA NGG 2 0.8523 Tier II
7 NC_000013.11 13 70029432 + KLHL1 NNNCTTCAGCCAGAAAAGAG NGG 2 0.6667 Tier II
8 NC_000012.12 12 45049337 + DBX2 NNNCTTCAAAAAGAACAGAG NGG 2 0.65 Tier II
9 NC_000008.11 8 27740177 + CCDC25 NNNCTGCAACCAGAAAAGAG NGG 2 0.5714 Tier II
10 NC_000020.11 20 51719266 + ATP9A NNNCTTCAACAAGAGCAGAG NGG 2 0.4875 Tier II
11 NC_000001.11 1 212089566 - DTL NNNATTCAACCAGTACAGAG NGG 2 0.4491 Tier II
12 NC_000003.12 3 115024741 + ZBTB20 NNNCTTCAATCACAACAGAG NGG 2 0.3963 Tier II
13 NC_000020.11 20 9835561 - PAK5 NNNCTTCATCCAGAGCAGAG NGG 2 0.39 Tier II
14 NC_000018.10 18 61871475 + RNF152 NNNCTTAAACCAGAACACAG NGG 2 0.3571 Tier II
15 NC_000004.12 4 23937132 + PPARGC1A NNNCTTTTACCAGAACAGAG NGG 2 0.3482 Tier II
16 NC_000023.11 X 55054985 + PAGE2B NNNTTTCAACCACAACAGAG NGG 2 0.3368 Tier II
17 NC_000002.12 2 9385412 + ASAP2 NNNCTTCATCCAGAACAGTG NGG 2 0.3231 Tier II
18 NC_000004.12 4 76058877 + ART3 NNNCCTCAACCAGAATAGAG NGG 2 0.3077 Tier II
19 NC_000001.11 1 5978046 + NPHP4 NNNCTTCAACCAGAGCAGAG NAG 2 0.1685 Tier II
20 NC_000004.12 4 141112561 - RNF150 NNNCTACAACCAGAAGAGAG NGG 2 0.1333 Tier II
21 NC_000010.11 10 116903128 - SHTN1 NNNCTTCCACTAGAACAGAG NGG 2 0.1319 Tier II
22 NC_000010.11 10 116903128 - ENO4 NNNCTTCCACTAGAACAGAG NGG 2 0.1319 Tier II
23 NC_000011.10 11 5632281 + TRIM34 NNNCTTTAACCAGAAGAGAG NGG 2 0.125 Tier II
24 NC_000011.10 11 5632281 + TRIM5 NNNCTTTAACCAGAAGAGAG NGG 2 0.125 Tier II
25 NC_000012.12 12 110529940 - RAD9B NNNCTTCCACCAGAACTGAG NGG 2 0.0571 Tier II
26 NC_000002.12 2 45884786 + PRKCE NNNCTTCAACCAGCACAGAG NGA 2 0.0149 Tier II
27 NC_000002.12 2 205763059 - NRP2 NNNCTTCAAACAGAACAGAG NGT 2 0.014 Tier II
28 NC_000020.11 20 19250607 + SLC24A3 NNNCTTCAACCAGAAGAGAG NTG 2 0.006 Tier II
29 NC_000023.11 X 55054985 + HDGFL3P1 NNNTTTCAACCACAACAGAG NGG 2 0.3368 Tier III
30 NC_000002.12 2 9385412 + LOC124907730 NNNCTTCATCCAGAACAGTG NGG 2 0.3231 Tier III
31 NC_000001.11 1 88467006 - LOC105378839 NNNCCTCAACCAGACCAGAG NGG 2 0.2727 Tier III
32 NC_000004.12 4 171043118 - LINC02431 NNNCCTCAACCAGCACAGAG NGG 2 0.2143 Tier III
33 NC_000004.12 4 3960771 - LOC105374358 NNNCTTCAACCAGAGCAGAG NAG 2 0.1685 Tier III
34 NC_000007.14 7 130928183 + LINC-PINT NNNCTTCAACAAGCACAGAG NGG 2 0.1607 Tier III
35 NC_000001.11 1 151941126 - LOC124904421 NNNCTTCTACCATAACAGAG NGG 2 0.1286 Tier III
36 NC_000003.12 3 28673214 - LINC00693 NNNCTTCAAACAGAACAGAG NTG 2 0.0338 Tier III
37 NC_000001.11 1 84003293 + LOC124904205 NNNCTTCTACCAGAACAGAG NGA 2 0.0298 Tier III
38 NC_000020.11 20 19250607 + SLC24A3-AS1 NNNCTTCAACCAGAAGAGAG NTG 2 0.006 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000074.6 8 76931467 + Nr3c2 NNNCTGCAACCAGAACAGAA NGG 2 0.5357 Tier II
2 NC_000072.6 6 90629211 - Slc41a3 NNNCTTCAACCAGAACAGTA NGG 2 0.5048 Tier II
3 NC_000081.6 15 39903009 + Lrp12 NNNCTTCCATCAGAACAGAG NGG 2 0.4034 Tier II
4 NC_000079.6 13 77804909 + Fam172a NNNCATAAACCAGAACAGAG NGG 2 0.375 Tier II
5 NC_000067.6 1 125780290 - Gpr39 NNNCTGCACCCAGAACAGAG NGG 2 0.3265 Tier II
6 NC_000075.6 9 109651961 + Fbxw25 NNNCTTCACCCAGGACAGAG NGG 2 0.2667 Tier II
7 NC_000070.6 4 134543776 + Selenon NNNCTTCTACCAGAACAGTG NGG 2 0.2308 Tier II
8 NC_000076.6 10 45705211 - Hace1 NNNCTTCAACCAGAACACAC NGG 2 0.2041 Tier II
9 NC_000070.6 4 44677627 - Pax5 NNNCTTCAACAAGAACAGAG NAG 2 0.1944 Tier II
10 NC_000073.6 7 88449409 - Rab38 NNNCTTCAAGCAGAACAGAC NGG 2 0.1667 Tier II
11 NC_000078.6 12 113055839 - Pacs2 NNNCTTAAACCAGCACAGAG NGG 2 0.1607 Tier II
12 NC_000075.6 9 62451555 - Coro2b NNNCTTCAACCAGAAGAGAA NGG 2 0.1442 Tier II
13 NC_000073.6 7 43885794 - Klk4 NNNCTTCAAACAGAACTGAG NGG 2 0.1156 Tier II
14 NC_000073.6 7 128708077 + Mcmbp NNNCTTCAACCAGAACTGAT NGG 2 0.0933 Tier II
15 NC_000077.6 11 12391289 + Cobl NNNCTTCAACCAGACTAGAG NGG 2 0.0839 Tier II
16 NC_000086.7 X 106046073 - Atp7a NNNCTTCAACCATAACAGAG NAG 2 0.0778 Tier II
17 NC_000072.6 6 47079111 - Cntnap2 NNNCTTCAACAAGAACAGAG NGA 2 0.0521 Tier II
18 NC_000085.6 19 6069207 - Vps51 NNNCTTCAACCAGCAGAGAG NGG 2 0.033 Tier II
19 NC_000072.6 6 34649590 - Cald1 NNNCTTCAACCAGGACAGAG NTG 2 0.0182 Tier II
20 NC_000079.6 13 20481847 - Elmo1 NNNCTTCAACCAGAACTGAG NGT 2 0.0022 Tier II
21 NC_000079.6 13 110885844 - 4930526H09Rik NNNCTTCAACAAGAACAGAG NGT 2 0.0121 Tier III
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Other clones with same target sequence:

(none)