Construct: sgRNA BRDN0001144933

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TGCCATCCTGAAAACAAACA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FN3KRP (79672)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
78003

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000017.11 17 82718961 + FN3KRP NNNCATCCTGAAAACAAACA NGG 0 1.0 Tier I
2 NC_000013.11 13 24681620 + ATP12A NNNCATCGTGAAAACAGACA NGG 2 0.1134 Tier I
3 NC_000002.12 2 224802051 + DOCK10 NNNCATCCTGAAAACAAAAA NAG 2 0.1111 Tier I
4 NC_000016.10 16 1583397 + IFT140 NNNCATCCTGAAAAGAACCA NGG 2 0.0095 Tier I
5 NC_000017.11 17 33783674 - ASIC2 NNNTATTCTGAAAACAAACA NGG 2 0.65 Tier II
6 NC_000002.12 2 153603988 - GALNT13 NNNAATACTGAAAACAAACA NGG 2 0.6316 Tier II
7 NC_000002.12 2 197191537 + ANKRD44 NNNCACCCTGAAAACAAACT NGG 2 0.5455 Tier II
8 NC_000002.12 2 231792043 - COPS7B NNNCATCTGGAAAACAAACA NGG 2 0.5417 Tier II
9 NC_000004.12 4 52016395 + LRRC66 NNNAAGCCTGAAAACAAACA NGG 2 0.4812 Tier II
10 NC_000022.11 22 46968745 - TBC1D22A NNNCATCCAGAAAACAAAAA NGG 2 0.398 Tier II
11 NC_000009.12 9 36956027 + PAX5 NNNCACCCTCAAAACAAACA NGG 2 0.3636 Tier II
12 NC_000011.10 11 92603910 + FAT3 NNNGATCCTGAAAACAAACT NGG 2 0.3 Tier II
13 NC_000011.10 11 70728628 + SHANK2 NNNCTTCCTGGAAACAAACA NGG 2 0.2364 Tier II
14 NC_000013.11 13 49450670 - SETDB2 NNNCATGCTGAAAACAAAAA NGG 2 0.2017 Tier II
15 NC_000013.11 13 49450670 - SETDB2-PHF11 NNNCATGCTGAAAACAAAAA NGG 2 0.2017 Tier II
16 NC_000004.12 4 168127110 + ANXA10 NNNCATACTGACAACAAACA NGG 2 0.1974 Tier II
17 NC_000006.12 6 117527133 + DCBLD1 NNNCATCCTCAAAGCAAACA NGG 2 0.1867 Tier II
18 NC_000004.12 4 47874946 - NFXL1 NNNCATCCTGAATTCAAACA NGG 2 0.16 Tier II
19 NC_000020.11 20 14540209 - MACROD2 NNNCATCCTGAATGCAAACA NGG 2 0.14 Tier II
20 NC_000012.12 12 119010748 - SRRM4 NNNCTTCCTGATAACAAACA NGG 2 0.1212 Tier II
21 NC_000020.11 20 18657337 + DTD1 NNNCATGCTGAAAAAAAACA NGG 2 0.1046 Tier II
22 NC_000006.12 6 136764981 - MAP3K5 NNNGATCCTGAAAACACACA NGG 2 0.0882 Tier II
23 NC_000001.11 1 235477157 + B3GALNT2 NNNCATCCTGAACACAAGCA NGG 2 0.0842 Tier II
24 NC_000009.12 9 36576011 - MELK NNNCATCCTAAAAACAAACA NGA 2 0.0648 Tier II
25 NC_000016.10 16 57661625 + ADGRG1 NNNAATCCTGAAAACAAACA NGA 2 0.0585 Tier II
26 NC_000009.12 9 18308862 + ADAMTSL1 NNNCATCCTGAAACCAAACC NGG 2 0.0487 Tier II
27 NC_000017.11 17 4184634 + ANKFY1 NNNCATCCTGAAAACACACA NAG 2 0.0458 Tier II
28 NC_000003.12 3 36449265 + STAC NNNCATCCTGAAAACAACCC NGG 2 0.0433 Tier II
29 NC_000019.10 19 283112 - PLPP2 NNNAATCCTGAAAAGAAACA NGG 2 0.0421 Tier II
30 NC_000012.12 12 70589696 - PTPRB NNNCATTCTGAAAACAAACA NTG 2 0.0317 Tier II
31 NC_000011.10 11 88056268 - RAB38 NNNCATCCTGAAGACAAACA NTG 2 0.0254 Tier II
32 NC_000006.12 6 83053862 - UBE3D NNNCCTCCTGAAAAGAAACA NGG 2 0.025 Tier II
33 NC_000003.12 3 183051214 - MCCC1 NNNCACCCTGAAAACAAACA NGC 2 0.0202 Tier II
34 NC_000013.11 13 25978418 + ATP8A2 NNNCAGCCTGAAAACAAACA NGC 2 0.0127 Tier II
35 NC_000003.12 3 2306573 - CNTN4 NNNCATCCTGAAAAAAAACA NTG 2 0.0087 Tier II
36 NC_000002.12 2 109237790 + RANBP2 NNNCATCCTGAAAACAAACA NAA 2 0.0 Tier II
37 NC_000002.12 2 109237790 + SH3RF3 NNNCATCCTGAAAACAAACA NAA 2 0.0 Tier II
38 NC_000003.12 3 151112726 - MED12L NNNCATCCTGAAAACAAACA NTT 2 0.0 Tier II
39 NC_000008.11 8 8851163 - MFHAS1 NNNCATCCTGAAAACCACCA NGG 2 0.0 Tier II
40 NC_000006.12 6 30814561 - LINC00243 NNNCATCTTAAAAACAAACA NGG 2 0.8167 Tier III
41 NC_000008.11 8 63950251 - LINC01414 NNNAATCCAGAAAACAAACA NGG 2 0.782 Tier III
42 NC_000004.12 4 47874946 - LOC101927179 NNNCATCCTGAATTCAAACA NGG 2 0.16 Tier III
43 NC_000004.12 4 100672503 + LINC01216 NNNCATACTGAAAACAACCA NGG 2 0.1429 Tier III
44 NC_000014.9 14 98227674 + LOC105370656 NNNCAGCCTGAAACCAAACA NGG 2 0.1224 Tier III
45 NC_000007.14 7 19928794 + MACC1-OT1 NNNCATCCTGAAATCAACCA NGG 2 0.1016 Tier III
46 NC_000013.11 13 34454801 + LINC00457 NNNCATCCTGAAAACAAACC NAG 2 0.0589 Tier III
47 NC_000013.11 13 34454801 + LINC02343 NNNCATCCTGAAAACAAACC NAG 2 0.0589 Tier III
48 NC_000003.12 3 36449265 + LOC124906227 NNNCATCCTGAAAACAACCC NGG 2 0.0433 Tier III
49 NC_000002.12 2 188234620 - LINC01090 NNNCATTCTGAAAACAAACA NTG 2 0.0317 Tier III
50 NC_000016.10 16 1583397 + LOC105371046 NNNCATCCTGAAAAGAACCA NGG 2 0.0095 Tier III
51 NC_000003.12 3 154068704 + ARHGEF26-AS1 NNNCATCCTGAAAACAAACA NCC 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000079.6 13 97185680 + Hexb NNNCATCCTGCAAACAAACA NGG 1 0.4 Tier I
2 NC_000075.6 9 99299796 + Cep70 NNNCATACTGAAAACAAACC NGG 2 0.1705 Tier I
3 NC_000071.6 5 123961212 - Ccdc62 NNNCATCCTGAAAACAAGCC NGG 2 0.0909 Tier I
4 NC_000075.6 9 19647534 + Zfp317 NNNCATCCTGAAAACACACA NGA 2 0.0123 Tier I
5 NC_000079.6 13 109144502 - Pde4d NNNAAACCTGAAAACAAACA NGG 2 0.7298 Tier II
6 NC_000082.6 16 8726778 + Usp7 NNNCATCCAGGAAACAAACA NGG 2 0.6036 Tier II
7 NC_000079.6 13 32112987 + Gmds NNNAATCCTGAAGACAAACA NGG 2 0.5492 Tier II
8 NC_000068.7 2 42400757 + Lrp1b NNNTCTCCTGAAAACAAACA NGG 2 0.4 Tier II
9 NC_000074.6 8 81400636 - Inpp4b NNNCATCTTCAAAACAAACA NGG 2 0.35 Tier II
10 NC_000079.6 13 32815690 - Wrnip1 NNNCATCTTGCAAACAAACA NGG 2 0.35 Tier II
11 NC_000077.6 11 68293434 - Ntn1 NNNTATCCTGAAAACAAAAA NGG 2 0.3429 Tier II
12 NC_000072.6 6 104633434 - Cntn6 NNNCATCATGAAAACAAAAA NGG 2 0.2786 Tier II
13 NC_000086.7 X 51410016 - Hs6st2 NNNCATCCTCAAGACAAACA NGG 2 0.2609 Tier II
14 NC_000083.6 17 65408364 - Tmem232 NNNCATCGTCAAAACAAACA NGG 2 0.2571 Tier II
15 NC_000082.6 16 92636652 - Runx1 NNNCACCCTGAAAACAAACA NAG 2 0.2357 Tier II
16 NC_000071.6 5 34347954 + Rnf4 NNNCATTCTGAACACAAACA NGG 2 0.1711 Tier II
17 NC_000075.6 9 28165270 + Opcml NNNCATCCTGAAGAAAAACA NGG 2 0.1449 Tier II
18 NC_000086.7 X 81089339 - Tmem47 NNNCATGCTGTAAACAAACA NGG 2 0.1448 Tier II
19 NC_000071.6 5 102017743 + Wdfy3 NNNCATCCTGAAGCCAAACA NGG 2 0.1398 Tier II
20 NC_000070.6 4 130946924 - Matn1 NNNCATCCTCTAAACAAACA NGG 2 0.1231 Tier II
21 NC_000067.6 1 129603006 - Thsd7b NNNCATCATGAAAACACACA NGG 2 0.1147 Tier II
22 NC_000075.6 9 75310062 + Gnb5 NNNCATCCTGAAAACAACCT NGG 2 0.1143 Tier II
23 NC_000077.6 11 97863346 + Fbxo47 NNNCATCCTGAAAAAAAAAA NGG 2 0.0952 Tier II
24 NC_000068.7 2 127803399 + Bub1 NNNCTTCCTGAAAACAAACA NAG 2 0.0943 Tier II
25 NC_000072.6 6 28897435 + Snd1 NNNCATCCTGAAAGCAGACA NGG 2 0.0824 Tier II
26 NC_000067.6 1 140596142 - Kcnt2 NNNCATCGTGAAAACAAAGA NGG 2 0.0804 Tier II
27 NC_000077.6 11 103016773 - Dcakd NNNCATCCTGATAACAAACC NGG 2 0.0758 Tier II
28 NC_000074.6 8 76935041 + Nr3c2 NNNCATCCTGAAAAAAAACA NAG 2 0.0576 Tier II
29 NC_000067.6 1 24349763 - Col19a1 NNNCATCCTGAAAAGAAACA NGG 1 0.05 Tier II
30 NC_000070.6 4 57748578 - Pakap NNNCATCCTGCAAACAAAGA NGG 2 0.05 Tier II
31 NC_000072.6 6 77752355 + Ctnna2 NNNCATCATGAAAATAAACA NGG 2 0.0433 Tier II
32 NC_000076.6 10 29349935 + Rnf146 NNNCAGCCTGAAAACAAACA NGA 2 0.0397 Tier II
33 NC_000073.6 7 144412514 - Shank2 NNNCATCCTGAAACCACACA NGG 2 0.0378 Tier II
34 NC_000086.7 X 165135524 - Glra2 NNNCAGCCTGAAAAGAAACA NGG 2 0.0286 Tier II
35 NC_000068.7 2 165438680 + Slc13a3 NNNCATCCTGAACACATACA NGG 2 0.0281 Tier II
36 NC_000082.6 16 41624248 + Lsamp NNNCATCCTGAAAAGAATCA NGG 2 0.025 Tier II
37 NC_000076.6 10 23246432 + Eya4 NNNCATCCTGAAAACAAACT NTG 2 0.0234 Tier II
38 NC_000068.7 2 50144869 + Lypd6 NNNCATCCTGAAAACAAACA NGT 1 0.0161 Tier II
39 NC_000086.7 X 18415282 + Dipk2b NNNCATCCTGAAACCAAACA NGA 2 0.0149 Tier II
40 NC_000085.6 19 17362368 + Gcnt1 NNNGATCCTGAAAACAAACA NGC 2 0.0111 Tier II
41 NC_000070.6 4 131946799 + Epb41 NNNCATCCTGAAAAGGAACA NGG 2 0.0096 Tier II
42 NC_000068.7 2 41937471 + Lrp1b NNNCATCCTGAAAACAATCA NGT 2 0.0081 Tier II
43 NC_000080.6 14 16965541 + Rarb NNNCATCCTGAACACAAACA NGC 2 0.0047 Tier II
44 NC_000078.6 12 57676138 + Ttc6 NNNCATCCTGAAACCAAACA NGT 2 0.0035 Tier II
45 NC_000079.6 13 111536558 - Gm15290 NNNCATACTAAAAACAAACA NGG 2 0.7 Tier III
46 NC_000079.6 13 32112987 + Gm35672 NNNAATCCTGAAGACAAACA NGG 2 0.5492 Tier III
47 NC_000074.6 8 26280372 - Gm31727 NNNCAGCCAGAAAACAAACA NGG 2 0.5306 Tier III
48 NC_000075.6 9 99299796 + 4930422M22Rik NNNCATACTGAAAACAAACC NGG 2 0.1705 Tier III
49 NC_000073.6 7 64065285 + Gm20670 NNNCATCCTGAAAACAAAAA NAG 2 0.1111 Tier III
50 NC_000067.6 1 140596142 - 4930590L20Rik NNNCATCGTGAAAACAAAGA NGG 2 0.0804 Tier III
51 NC_000067.6 1 102468155 - Gm53054 NNNCATCCTGTACACAAACA NGG 2 0.0648 Tier III
52 NC_000070.6 4 57748578 - Gm35099 NNNCATCCTGCAAACAAAGA NGG 2 0.05 Tier III
53 NC_000078.6 12 12992237 - Gm35208 NNNCATCCTGAACACAAACA NTG 2 0.0082 Tier III
54 NC_000073.6 7 94362530 + Gm32647 NNNCATCTTGAAAACTAACA NGG 2 0.0 Tier III
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Other clones with same target sequence:

(none)