Construct: sgRNA BRDN0001144996
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ACAATATTACCATTGACTTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- NEK5 (341676)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76118
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000013.11 |
13 |
52093061 |
+ |
NEK5 |
NNNATATTACCATTGACTTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000002.12 |
2 |
197136296 |
- |
ANKRD44 |
NNNATATCATCATTGACTTG |
NGG |
2 |
0.9412 |
Tier II |
| 3 |
NC_000005.10 |
5 |
50699563 |
+ |
PARP8 |
NNNATATTATCACTGACTTG |
NGG |
2 |
0.743 |
Tier II |
| 4 |
NC_000002.12 |
2 |
2188232 |
- |
MYT1L |
NNNATATTACCATTAACTTT |
NGG |
2 |
0.6588 |
Tier II |
| 5 |
NC_000001.11 |
1 |
14669968 |
+ |
KAZN |
NNNAGATTACCATTGACTTG |
NGG |
1 |
0.64 |
Tier II |
| 6 |
NC_000002.12 |
2 |
186638043 |
+ |
ITGAV |
NNNATATTACAAATGACTTG |
NGG |
2 |
0.5192 |
Tier II |
| 7 |
NC_000008.11 |
8 |
112484678 |
- |
CSMD3 |
NNNTTGTTACCATTGACTTG |
NGG |
2 |
0.4545 |
Tier II |
| 8 |
NC_000010.11 |
10 |
95767757 |
- |
ENTPD1 |
NNNATATTTCCAATGACTTG |
NGG |
2 |
0.4154 |
Tier II |
| 9 |
NC_000016.10 |
16 |
55941890 |
- |
CES5A |
NNNATAGTACCATTGACTTT |
NGG |
2 |
0.4118 |
Tier II |
| 10 |
NC_000007.14 |
7 |
154563767 |
+ |
DPP6 |
NNNTTATTCCCATTGACTTG |
NGG |
2 |
0.3636 |
Tier II |
| 11 |
NC_000023.11 |
X |
109414419 |
- |
GUCY2F |
NNNATATGACCATTGACTTC |
NGG |
2 |
0.3143 |
Tier II |
| 12 |
NC_000010.11 |
10 |
55419489 |
- |
PCDH15 |
NNNCTATTAACATTGACTTG |
NGG |
2 |
0.3059 |
Tier II |
| 13 |
NC_000002.12 |
2 |
197162173 |
- |
ANKRD44 |
NNNAGATTAGCATTGACTTG |
NGG |
2 |
0.2489 |
Tier II |
| 14 |
NC_000017.11 |
17 |
19316398 |
+ |
EPN2 |
NNNTTATTACCAGTGACTTG |
NGG |
2 |
0.166 |
Tier II |
| 15 |
NC_000011.10 |
11 |
88578132 |
- |
GRM5 |
NNNATAATACCATTTACTTG |
NGG |
2 |
0.125 |
Tier II |
| 16 |
NC_000007.14 |
7 |
139574715 |
+ |
HIPK2 |
NNNATCTTACCCTTGACTTG |
NGG |
2 |
0.1196 |
Tier II |
| 17 |
NC_000004.12 |
4 |
92828111 |
- |
GRID2 |
NNNATATTACCAATTACTTG |
NGG |
2 |
0.0989 |
Tier II |
| 18 |
NC_000004.12 |
4 |
185373824 |
- |
LRP2BP |
NNNATATTACCGTTGACTTG |
NGA |
2 |
0.0502 |
Tier II |
| 19 |
NC_000004.12 |
4 |
185373824 |
- |
SNX25 |
NNNATATTACCGTTGACTTG |
NGA |
2 |
0.0502 |
Tier II |
| 20 |
NC_000003.12 |
3 |
131013813 |
+ |
ATP2C1 |
NNNATATTACCATTAACTTG |
NTG |
2 |
0.0367 |
Tier II |
| 21 |
NC_000014.9 |
14 |
79774341 |
- |
NRXN3 |
NNNATATTACCATTGACTTG |
NTC |
2 |
0.0 |
Tier II |
| 22 |
NC_000001.11 |
1 |
169222517 |
- |
NME7 |
NNNATATTACCATTTCCTTG |
NGG |
2 |
0.0 |
Tier II |
| 23 |
NC_000021.9 |
21 |
29637510 |
- |
GRIK1 |
NNNCTATTACCATTGTCTTG |
NGG |
2 |
0.0 |
Tier II |
| 24 |
NC_000010.11 |
10 |
95767757 |
- |
ENTPD1-AS1 |
NNNATATTTCCAATGACTTG |
NGG |
2 |
0.4154 |
Tier III |
| 25 |
NC_000004.12 |
4 |
16470292 |
- |
LOC105374505 |
NNNATATTACTATAGACTTG |
NGG |
2 |
0.1905 |
Tier III |
| 26 |
NC_000011.10 |
11 |
112500945 |
+ |
LINC02763 |
NNNATATCACCATGGACTTG |
NGG |
2 |
0.0 |
Tier III |
| 27 |
NC_000005.10 |
5 |
152002602 |
+ |
LINC01933 |
NNNATATTACCTTGGACTTG |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000085.6 |
19 |
27414161 |
- |
Pum3 |
NNNATTTTAACATTGACTTG |
NGG |
2 |
0.619 |
Tier II |
| 2 |
NC_000071.6 |
5 |
20479785 |
+ |
Magi2 |
NNNTTATTATCATTGACTTG |
NGG |
2 |
0.5989 |
Tier II |
| 3 |
NC_000082.6 |
16 |
72753188 |
- |
Robo1 |
NNNAAATTATCATTGACTTG |
NGG |
2 |
0.4706 |
Tier II |
| 4 |
NC_000067.6 |
1 |
134308133 |
+ |
Ppfia4 |
NNNATCTTACCATTGACTTG |
NGG |
1 |
0.4545 |
Tier II |
| 5 |
NC_000069.6 |
3 |
26804800 |
- |
Spata16 |
NNNATATTACCATTGAATTA |
NGG |
2 |
0.4375 |
Tier II |
| 6 |
NC_000070.6 |
4 |
9655476 |
+ |
Asph |
NNNATATTAACATTGATTTG |
NGG |
2 |
0.4044 |
Tier II |
| 7 |
NC_000075.6 |
9 |
59549508 |
- |
Hexa |
NNNAAATTTCCATTGACTTG |
NGG |
2 |
0.3 |
Tier II |
| 8 |
NC_000081.6 |
15 |
34310992 |
+ |
Matn2 |
NNNAAAGTACCATTGACTTG |
NGG |
2 |
0.2941 |
Tier II |
| 9 |
NC_000080.6 |
14 |
42423898 |
+ |
Gm2959 |
NNNATCTTACCATTGACTTC |
NGG |
2 |
0.1948 |
Tier II |
| 10 |
NC_000069.6 |
3 |
123007052 |
+ |
Myoz2 |
NNNAGATTACCATTTACTTG |
NGG |
2 |
0.0914 |
Tier II |
| 11 |
NC_000086.7 |
X |
47842747 |
+ |
Smarca1 |
NNNATATTACCATTGGCTTG |
NAG |
2 |
0.0499 |
Tier II |
| 12 |
NC_000073.6 |
7 |
115566203 |
+ |
Sox6 |
NNNATATTACTATTGACTTG |
NGA |
2 |
0.0214 |
Tier II |
| 13 |
NC_000072.6 |
6 |
64394753 |
- |
Grid2 |
NNNATATTACCATTGACTTC |
NGT |
2 |
0.0069 |
Tier II |
| 14 |
NC_000086.7 |
X |
135622668 |
- |
Prame |
NNNATAATACCATGGACTTG |
NGG |
2 |
0.0 |
Tier II |
| 15 |
NC_000076.6 |
10 |
92276975 |
- |
Gm20757 |
NNNATATAATCATTGACTTG |
NGG |
2 |
0.7529 |
Tier III |
| 16 |
NC_000076.6 |
10 |
3301051 |
- |
Gm29826 |
NNNATGTTACCTTTGACTTG |
NGG |
2 |
0.2381 |
Tier III |
Other clones with same target sequence:
(none)
