Construct: sgRNA BRDN0001145013
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GATGTCTACATCGTTCAGAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PRPS1L1 (221823)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75838
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000007.14 |
7 |
18027613 |
- |
PRPS1L1 |
NNNGTCTACATCGTTCAGAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000023.11 |
X |
107639343 |
+ |
PRPS1 |
NNNGTCTACATTGTTCAGAG |
NGG |
1 |
0.5385 |
Tier I |
| 3 |
NC_000023.11 |
X |
12799255 |
+ |
PRPS2 |
NNNGTCTACATCATCCAGAG |
NGG |
2 |
0.2517 |
Tier I |
| 4 |
NC_000009.12 |
9 |
125150818 |
+ |
PPP6C |
NNNGTCTACATCATTCAGAG |
NGG |
1 |
0.9231 |
Tier II |
| 5 |
NC_000010.11 |
10 |
6411158 |
+ |
PRKCQ |
NNNGTCTACATAGTTCATAG |
NGG |
2 |
0.4762 |
Tier II |
| 6 |
NC_000004.12 |
4 |
99826272 |
+ |
DAPP1 |
NNNGTCTAGATAGTTCAGAG |
NGG |
2 |
0.4422 |
Tier II |
| 7 |
NC_000013.11 |
13 |
25454435 |
- |
ATP8A2 |
NNNATCTTCATCGTTCAGAG |
NGG |
2 |
0.3857 |
Tier II |
| 8 |
NC_000002.12 |
2 |
73051817 |
+ |
SFXN5 |
NNNGTCTGCATCCTTCAGAG |
NGG |
2 |
0.3088 |
Tier II |
| 9 |
NC_000008.11 |
8 |
23305169 |
+ |
LOXL2 |
NNNGTCTGCATCGCTCAGAG |
NGG |
2 |
0.2095 |
Tier II |
| 10 |
NC_000001.11 |
1 |
232967302 |
+ |
NTPCR |
NNNGTCTACATGCTTCAGAG |
NGG |
2 |
0.1871 |
Tier II |
| 11 |
NC_000002.12 |
2 |
55885455 |
- |
EFEMP1 |
NNNGTCTACATAGTTCAGAG |
NGA |
2 |
0.0496 |
Tier II |
| 12 |
NC_000001.11 |
1 |
7886459 |
- |
UTS2 |
NNNGTCTACATCGTTCAGAA |
NGC |
2 |
0.0208 |
Tier II |
| 13 |
NC_000009.12 |
9 |
125150818 |
+ |
PRPS1P2 |
NNNGTCTACATCATTCAGAG |
NGG |
1 |
0.9231 |
Tier III |
| 14 |
NC_000011.10 |
11 |
83382289 |
+ |
CCDC90B-AS1 |
NNNGTCTCCATCGTTCAGAA |
NGG |
2 |
0.4018 |
Tier III |
| 15 |
NC_000015.10 |
15 |
39159609 |
- |
LOC105370777 |
NNNGGCTACATTGTTCAGAG |
NGG |
2 |
0.3446 |
Tier III |
| 16 |
NC_000018.10 |
18 |
77518991 |
+ |
LOC107985172 |
NNNGTCTACATGGTTCAGAG |
NAG |
2 |
0.1152 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000078.6 |
12 |
34985058 |
+ |
Prps1l1 |
NNNGTCTACATCGTTCAGAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000078.6 |
12 |
57238596 |
+ |
Prps1l3 |
NNNGTCTACATTGTTCAGAG |
NGG |
1 |
0.5385 |
Tier I |
| 3 |
NC_000086.7 |
X |
140465279 |
+ |
Prps1 |
NNNGTCTACATTGTTCAAAG |
NGG |
2 |
0.3728 |
Tier I |
| 4 |
NC_000086.7 |
X |
167374139 |
- |
Prps2 |
NNNGTCTACATCATCCAGAG |
NGG |
2 |
0.2517 |
Tier I |
| 5 |
NC_000068.7 |
2 |
22323630 |
- |
Myo3a |
NNNGTCTACATCCTTCAGAC |
NGG |
2 |
0.1805 |
Tier I |
| 6 |
NC_000078.6 |
12 |
57238596 |
+ |
Mipol1 |
NNNGTCTACATTGTTCAGAG |
NGG |
1 |
0.5385 |
Tier II |
| 7 |
NC_000068.7 |
2 |
49843402 |
- |
Lypd6b |
NNNCTCTACATAGTTCAGAG |
NGG |
2 |
0.3782 |
Tier II |
| 8 |
NC_000071.6 |
5 |
148027683 |
+ |
Mtus2 |
NNNGTCCACATCGTTCAGTG |
NGG |
2 |
0.3702 |
Tier II |
| 9 |
NC_000076.6 |
10 |
61765253 |
+ |
Macroh2a2 |
NNNTTCTAAATCGTTCAGAG |
NGG |
2 |
0.3117 |
Tier II |
| 10 |
NC_000067.6 |
1 |
156437970 |
+ |
Soat1 |
NNNGGCTACATCCTTCAGAG |
NGG |
2 |
0.2695 |
Tier II |
| 11 |
NC_000077.6 |
11 |
59250499 |
- |
Wnt3a |
NNNGTCTACATTGTTCAGAG |
NGA |
2 |
0.0374 |
Tier II |
| 12 |
NC_000078.6 |
12 |
30243805 |
- |
Sntg2 |
NNNGTCTACATGGTTCAGAG |
NGA |
2 |
0.0309 |
Tier II |
| 13 |
NC_000078.6 |
12 |
34985058 |
+ |
Gm33864 |
NNNGTCTACATCGTTCAGAG |
NGG |
0 |
1.0 |
Tier III |
Other clones with same target sequence:
(none)
