Construct: sgRNA BRDN0001145037
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AAGCATACTCCAGTCGAACA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PRKACB (5567)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75960
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000001.11 |
1 |
84184114 |
- |
PRKACB |
NNNCATACTCCAGTCGAACA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000019.10 |
19 |
17162399 |
- |
MYO9B |
NNNCACAATCCAGTCGAACA |
NGG |
2 |
0.5909 |
Tier I |
| 3 |
NC_000008.11 |
8 |
101944456 |
- |
NCALD |
NNNCATACTCCAGTCGAGCA |
NGC |
2 |
0.0089 |
Tier II |
| 4 |
NC_000012.12 |
12 |
8854350 |
+ |
A2ML1 |
NNNCATACTCCAGTCCAACC |
NGG |
2 |
0.0 |
Tier II |
| 5 |
NC_000006.12 |
6 |
132884185 |
- |
LOC105378008 |
NNNCATACTCCAGTGGAACA |
NAG |
2 |
0.013 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000080.6 |
14 |
30870859 |
+ |
Stimate |
NNNCATACTCCAGTGGAAAA |
NGG |
2 |
0.0214 |
Tier I |
| 2 |
NC_000068.7 |
2 |
102148092 |
- |
Ldlrad3 |
NNNCATATTACAGTCGAACA |
NGG |
2 |
0.7583 |
Tier II |
| 3 |
NC_000084.6 |
18 |
45285424 |
+ |
Kcnn2 |
NNNAATTCTCCAGTCGAACA |
NGG |
2 |
0.3684 |
Tier II |
| 4 |
NC_000077.6 |
11 |
88351931 |
- |
Msi2 |
NNNCATACTCCAGTGGAACT |
NGG |
2 |
0.03 |
Tier II |
| 5 |
NC_000069.6 |
3 |
129283627 |
+ |
Enpep |
NNNCATACTCCATTCTAACA |
NGG |
2 |
0.0 |
Tier II |
| 6 |
NC_000080.6 |
14 |
35051467 |
- |
Grid1 |
NNNCATGCTCCAGTCTAACA |
NGG |
2 |
0.0 |
Tier II |
Other clones with same target sequence:
(none)
